Animal Cell Biotechnology: Methods and Protocols
Animal Cell Biotechnology: Methods and Protocols, Third Edition constitutes a comprehensive manual of state-of-the-art and new techniques for setting up mammalian cell lines for production of biopharmaceuticals, and for optimizing critical parameters for cell culture from lab to final production. Th...
Gespeichert in:
| Weitere Verfasser: | |
|---|---|
| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
Totowa, NJ
Humana Press
2014
|
| Ausgabe: | 3rd ed. 2014 |
| Schriftenreihe: | Methods in Molecular Biology, Methods and Protocols
1104 |
| Schlagworte: | |
| Online-Zugang: | UBR01 TUM01 Volltext |
| Zusammenfassung: | Animal Cell Biotechnology: Methods and Protocols, Third Edition constitutes a comprehensive manual of state-of-the-art and new techniques for setting up mammalian cell lines for production of biopharmaceuticals, and for optimizing critical parameters for cell culture from lab to final production. The volume is divided into five parts that reflect the processes required for different stages of production. In Part I, basic techniques for establishment of production cell lines are addressed, especially high-throughput synchronization, insect cell lines, transient gene and protein expression, DNA Profiling and Characterisation. Part II addresses tools for process and medium optimization as well as microcarrier technology while Part III covers monitoring of cell growth, viability and apoptosis, metabolic flux estimation, quenching methods as well as NMR-based techniques. Part IV details cultivation techniques, and Part V describes special applications, including vaccine production, baculovirus protein expression, chromatographic techniques for downstream as well as membrane techniques for virus separation. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Animal Cell Biotechnology: Methods and Protocols, Third Edition provides a compendium of techniques for scientists in industrial and research laboratories that use mammalian cells for biotechnology purposes |
| Beschreibung: | 1 Online-Ressource (XIV, 498 p. 83 illus., 28 illus. in color) |
| ISBN: | 9781627037334 |
| DOI: | 10.1007/978-1-62703-733-4 |
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| spelling | Animal Cell Biotechnology Methods and Protocols edited by Ralf Pörtner 3rd ed. 2014 Totowa, NJ Humana Press 2014 1 Online-Ressource (XIV, 498 p. 83 illus., 28 illus. in color) txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology, Methods and Protocols 1104 Animal Cell Biotechnology: Methods and Protocols, Third Edition constitutes a comprehensive manual of state-of-the-art and new techniques for setting up mammalian cell lines for production of biopharmaceuticals, and for optimizing critical parameters for cell culture from lab to final production. The volume is divided into five parts that reflect the processes required for different stages of production. In Part I, basic techniques for establishment of production cell lines are addressed, especially high-throughput synchronization, insect cell lines, transient gene and protein expression, DNA Profiling and Characterisation. Part II addresses tools for process and medium optimization as well as microcarrier technology while Part III covers monitoring of cell growth, viability and apoptosis, metabolic flux estimation, quenching methods as well as NMR-based techniques. Part IV details cultivation techniques, and Part V describes special applications, including vaccine production, baculovirus protein expression, chromatographic techniques for downstream as well as membrane techniques for virus separation. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Animal Cell Biotechnology: Methods and Protocols, Third Edition provides a compendium of techniques for scientists in industrial and research laboratories that use mammalian cells for biotechnology purposes Chemistry Biotechnology Cell Culture Cell culture Tierzelle (DE-588)4185509-7 gnd rswk-swf Biotechnologie (DE-588)4069491-4 gnd rswk-swf Tierzelle (DE-588)4185509-7 s Biotechnologie (DE-588)4069491-4 s 1\p DE-604 Pörtner, Ralf edt Erscheint auch als Druck-Ausgabe 978-1-62703-732-7 https://doi.org/10.1007/978-1-62703-733-4 Verlag URL des Erstveröffentlichers Volltext 1\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk |
| spellingShingle | Animal Cell Biotechnology Methods and Protocols Chemistry Biotechnology Cell Culture Cell culture Tierzelle (DE-588)4185509-7 gnd Biotechnologie (DE-588)4069491-4 gnd |
| subject_GND | (DE-588)4185509-7 (DE-588)4069491-4 |
| title | Animal Cell Biotechnology Methods and Protocols |
| title_auth | Animal Cell Biotechnology Methods and Protocols |
| title_exact_search | Animal Cell Biotechnology Methods and Protocols |
| title_full | Animal Cell Biotechnology Methods and Protocols edited by Ralf Pörtner |
| title_fullStr | Animal Cell Biotechnology Methods and Protocols edited by Ralf Pörtner |
| title_full_unstemmed | Animal Cell Biotechnology Methods and Protocols edited by Ralf Pörtner |
| title_short | Animal Cell Biotechnology |
| title_sort | animal cell biotechnology methods and protocols |
| title_sub | Methods and Protocols |
| topic | Chemistry Biotechnology Cell Culture Cell culture Tierzelle (DE-588)4185509-7 gnd Biotechnologie (DE-588)4069491-4 gnd |
| topic_facet | Chemistry Biotechnology Cell Culture Cell culture Tierzelle Biotechnologie |
| url | https://doi.org/10.1007/978-1-62703-733-4 |
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