Immunochemical Protocols:
This new third edition of a much praised classic has been updated and revised to provide both basic methods for producing and using antibodies and advanced techniques required by today's state-of-the art investigators. The basic sections of the book stress the first principles of antibody produ...
Gespeichert in:
Weitere Verfasser: | |
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Format: | Elektronisch E-Book |
Sprache: | English |
Veröffentlicht: |
Totowa, NJ
Humana Press
2005
|
Ausgabe: | Third Edition |
Schriftenreihe: | Methods In Molecular Biology™
295 |
Schlagworte: | |
Online-Zugang: | UBR01 TUM01 Volltext |
Zusammenfassung: | This new third edition of a much praised classic has been updated and revised to provide both basic methods for producing and using antibodies and advanced techniques required by today's state-of-the art investigators. The basic sections of the book stress the first principles of antibody production and guide the reader through the processes and pitfalls of immunochemistry. The advanced sections focus on specific applications and cover the major immunochemical techniques currently used in laboratories. New material includes techniques for making new monoclonal cell lines, for producing good quality monoclonal antibodies, immunocapture PCR, immunoaffinity purification, and immunoelectron microscopy in plant virus diagnosis. The authors provide full details of methods for antigen selection and preparation, antibody production, and reagent manufacture. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Up-to-date and highly practical, Immunochemical Protocols, Third Edition, takes both novice and experienced researchers from project initiation to final test format with a broad array of protocols suited to immediate use in the laboratory, as well as methods that can be successfully applied to entirely new systems and processes |
Beschreibung: | 1 Online-Ressource (X, 318 p. 22 illus) |
ISBN: | 9781592598731 |
DOI: | 10.1385/1592598730 |
Internformat
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Datensatz im Suchindex
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any_adam_object | |
author2 | Burns, Robert |
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author_facet | Burns, Robert |
building | Verbundindex |
bvnumber | BV044951356 |
callnumber-first | Q - Science |
callnumber-label | QR183 |
callnumber-raw | QR183.6 |
callnumber-search | QR183.6 |
callnumber-sort | QR 3183.6 |
callnumber-subject | QR - Microbiology |
classification_rvk | WC 4150 WC 5700 |
classification_tum | MED 460f CHE 810f CHE 880f |
collection | ZDB-2-PRO |
ctrlnum | (ZDB-2-PRO)978-1-59259-873-1 (OCoLC)850318007 (DE-599)BVBBV044951356 |
dewey-full | 616.07/9 572 |
dewey-hundreds | 600 - Technology (Applied sciences) 500 - Natural sciences and mathematics |
dewey-ones | 616 - Diseases 572 - Biochemistry |
dewey-raw | 616.07/9 572 |
dewey-search | 616.07/9 572 |
dewey-sort | 3616.07 19 |
dewey-tens | 610 - Medicine and health 570 - Biology |
discipline | Biologie Chemie Medizin |
doi_str_mv | 10.1385/1592598730 |
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isbn | 9781592598731 |
language | English |
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spelling | Immunochemical Protocols edited by Robert Burns Third Edition Totowa, NJ Humana Press 2005 1 Online-Ressource (X, 318 p. 22 illus) txt rdacontent c rdamedia cr rdacarrier Methods In Molecular Biology™ 295 This new third edition of a much praised classic has been updated and revised to provide both basic methods for producing and using antibodies and advanced techniques required by today's state-of-the art investigators. The basic sections of the book stress the first principles of antibody production and guide the reader through the processes and pitfalls of immunochemistry. The advanced sections focus on specific applications and cover the major immunochemical techniques currently used in laboratories. New material includes techniques for making new monoclonal cell lines, for producing good quality monoclonal antibodies, immunocapture PCR, immunoaffinity purification, and immunoelectron microscopy in plant virus diagnosis. The authors provide full details of methods for antigen selection and preparation, antibody production, and reagent manufacture. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Up-to-date and highly practical, Immunochemical Protocols, Third Edition, takes both novice and experienced researchers from project initiation to final test format with a broad array of protocols suited to immediate use in the laboratory, as well as methods that can be successfully applied to entirely new systems and processes Life Sciences Biochemistry, general Life sciences Biochemistry Immunchemie (DE-588)4161366-1 gnd rswk-swf Labortechnik (DE-588)4123602-6 gnd rswk-swf 1\p (DE-588)4143413-4 Aufsatzsammlung gnd-content Immunchemie (DE-588)4161366-1 s Labortechnik (DE-588)4123602-6 s b DE-604 Burns, Robert edt Erscheint auch als Druck-Ausgabe 9781588292742 https://doi.org/10.1385/1592598730 Verlag URL des Erstveröffentlichers Volltext 1\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk |
spellingShingle | Immunochemical Protocols Life Sciences Biochemistry, general Life sciences Biochemistry Immunchemie (DE-588)4161366-1 gnd Labortechnik (DE-588)4123602-6 gnd |
subject_GND | (DE-588)4161366-1 (DE-588)4123602-6 (DE-588)4143413-4 |
title | Immunochemical Protocols |
title_auth | Immunochemical Protocols |
title_exact_search | Immunochemical Protocols |
title_full | Immunochemical Protocols edited by Robert Burns |
title_fullStr | Immunochemical Protocols edited by Robert Burns |
title_full_unstemmed | Immunochemical Protocols edited by Robert Burns |
title_short | Immunochemical Protocols |
title_sort | immunochemical protocols |
topic | Life Sciences Biochemistry, general Life sciences Biochemistry Immunchemie (DE-588)4161366-1 gnd Labortechnik (DE-588)4123602-6 gnd |
topic_facet | Life Sciences Biochemistry, general Life sciences Biochemistry Immunchemie Labortechnik Aufsatzsammlung |
url | https://doi.org/10.1385/1592598730 |
work_keys_str_mv | AT burnsrobert immunochemicalprotocols |