Plant Proteomics: Methods and Protocols
The aim of Plant Proteomics: Methods and Protocols is to present up-- date methods and protocols used by recognized scientists in the world of plant proteomics. If this world was a very small one twenty-five years ago when the first papers were published, it has since experienced exponential growth,...
Gespeichert in:
| Weitere Verfasser: | , , , |
|---|---|
| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
Totowa, NJ
Humana Press
2007
|
| Schriftenreihe: | Methods in Molecular Biology
355 |
| Schlagworte: | |
| Online-Zugang: | UBR01 TUM01 URL des Erstveröffentlichers |
| Zusammenfassung: | The aim of Plant Proteomics: Methods and Protocols is to present up-- date methods and protocols used by recognized scientists in the world of plant proteomics. If this world was a very small one twenty-five years ago when the first papers were published, it has since experienced exponential growth, and in most countries around the world there are laboratories working on plant proteomics. Two-dimensional gel electrophoresis is still the basic method used, but it has been improved greatly with IPG in the first dimension (Chapter 13) and with new detection methods with fluorochromes (Chapters 14 and 15). Signi- cant progress has been achieved in protein extraction, which is particularly difficult with plant tissues containing phenols, proteases, and other secondary metabolites that interfere with proteins. Standard procedures have been op- mized (Chapters 1 and 2) for peculiar tissues (Chapters 3, 4, and 5) and cellular compartments (Chapters 6 to 10). These methods rely on improvements made in the solubilization of proteins from membranes (Chapters 11 and 12). Mass spectrometry was a revolution that permitted the high throughput identifi- tion of proteins separated by 2D gels (Chapters 19 and 20) but also from blue native 1D gels (Chapters 27 and 28) despite the fact that Edman sequencing can still be useful (Chapter 18). Associated with other techniques such as 2DLC or LC of intact proteins, mass spectrometry also permits the identification of polypeptides from complexes (Chapters 21 and 22) |
| Beschreibung: | 1 Online-Ressource (416 p. 135 illus) |
| ISBN: | 9781597452274 |
| DOI: | 10.1385/1597452270 |
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| 520 | |a The aim of Plant Proteomics: Methods and Protocols is to present up-- date methods and protocols used by recognized scientists in the world of plant proteomics. If this world was a very small one twenty-five years ago when the first papers were published, it has since experienced exponential growth, and in most countries around the world there are laboratories working on plant proteomics. Two-dimensional gel electrophoresis is still the basic method used, but it has been improved greatly with IPG in the first dimension (Chapter 13) and with new detection methods with fluorochromes (Chapters 14 and 15). Signi- cant progress has been achieved in protein extraction, which is particularly difficult with plant tissues containing phenols, proteases, and other secondary metabolites that interfere with proteins. Standard procedures have been op- mized (Chapters 1 and 2) for peculiar tissues (Chapters 3, 4, and 5) and cellular compartments (Chapters 6 to 10). These methods rely on improvements made in the solubilization of proteins from membranes (Chapters 11 and 12). Mass spectrometry was a revolution that permitted the high throughput identifi- tion of proteins separated by 2D gels (Chapters 19 and 20) but also from blue native 1D gels (Chapters 27 and 28) despite the fact that Edman sequencing can still be useful (Chapter 18). Associated with other techniques such as 2DLC or LC of intact proteins, mass spectrometry also permits the identification of polypeptides from complexes (Chapters 21 and 22) | ||
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Datensatz im Suchindex
| _version_ | 1804178539830837248 |
|---|---|
| any_adam_object | |
| author2 | Thiellement, Hervé Zivy, Michel Damerval, Catherine Méchin, Valérie |
| author2_role | edt edt edt edt |
| author2_variant | h t ht m z mz c d cd v m vm |
| author_facet | Thiellement, Hervé Zivy, Michel Damerval, Catherine Méchin, Valérie |
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| callnumber-first | Q - Science |
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| dewey-full | 580 572/.62 |
| dewey-hundreds | 500 - Natural sciences and mathematics |
| dewey-ones | 580 - Plants 572 - Biochemistry |
| dewey-raw | 580 572/.62 |
| dewey-search | 580 572/.62 |
| dewey-sort | 3580 |
| dewey-tens | 580 - Plants 570 - Biology |
| discipline | Biologie |
| doi_str_mv | 10.1385/1597452270 |
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| spelling | Plant Proteomics Methods and Protocols edited by Hervé Thiellement, Michel Zivy, Catherine Damerval, Valérie Méchin Totowa, NJ Humana Press 2007 1 Online-Ressource (416 p. 135 illus) txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology 355 The aim of Plant Proteomics: Methods and Protocols is to present up-- date methods and protocols used by recognized scientists in the world of plant proteomics. If this world was a very small one twenty-five years ago when the first papers were published, it has since experienced exponential growth, and in most countries around the world there are laboratories working on plant proteomics. Two-dimensional gel electrophoresis is still the basic method used, but it has been improved greatly with IPG in the first dimension (Chapter 13) and with new detection methods with fluorochromes (Chapters 14 and 15). Signi- cant progress has been achieved in protein extraction, which is particularly difficult with plant tissues containing phenols, proteases, and other secondary metabolites that interfere with proteins. Standard procedures have been op- mized (Chapters 1 and 2) for peculiar tissues (Chapters 3, 4, and 5) and cellular compartments (Chapters 6 to 10). These methods rely on improvements made in the solubilization of proteins from membranes (Chapters 11 and 12). Mass spectrometry was a revolution that permitted the high throughput identifi- tion of proteins separated by 2D gels (Chapters 19 and 20) but also from blue native 1D gels (Chapters 27 and 28) despite the fact that Edman sequencing can still be useful (Chapter 18). Associated with other techniques such as 2DLC or LC of intact proteins, mass spectrometry also permits the identification of polypeptides from complexes (Chapters 21 and 22) Life Sciences Plant Sciences Life sciences Plant science Botany Proteom (DE-588)4576155-3 gnd rswk-swf Genetik (DE-588)4071711-2 gnd rswk-swf Proteomanalyse (DE-588)4596545-6 gnd rswk-swf Pflanzen (DE-588)4045539-7 gnd rswk-swf 1\p (DE-588)4143413-4 Aufsatzsammlung gnd-content Pflanzen (DE-588)4045539-7 s Proteomanalyse (DE-588)4596545-6 s DE-604 Proteom (DE-588)4576155-3 s Genetik (DE-588)4071711-2 s b DE-604 Thiellement, Hervé edt Zivy, Michel edt Damerval, Catherine edt Méchin, Valérie edt Erscheint auch als Druck-Ausgabe 9781588296351 https://doi.org/10.1385/1597452270 Verlag URL des Erstveröffentlichers Volltext 1\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk |
| spellingShingle | Plant Proteomics Methods and Protocols Life Sciences Plant Sciences Life sciences Plant science Botany Proteom (DE-588)4576155-3 gnd Genetik (DE-588)4071711-2 gnd Proteomanalyse (DE-588)4596545-6 gnd Pflanzen (DE-588)4045539-7 gnd |
| subject_GND | (DE-588)4576155-3 (DE-588)4071711-2 (DE-588)4596545-6 (DE-588)4045539-7 (DE-588)4143413-4 |
| title | Plant Proteomics Methods and Protocols |
| title_auth | Plant Proteomics Methods and Protocols |
| title_exact_search | Plant Proteomics Methods and Protocols |
| title_full | Plant Proteomics Methods and Protocols edited by Hervé Thiellement, Michel Zivy, Catherine Damerval, Valérie Méchin |
| title_fullStr | Plant Proteomics Methods and Protocols edited by Hervé Thiellement, Michel Zivy, Catherine Damerval, Valérie Méchin |
| title_full_unstemmed | Plant Proteomics Methods and Protocols edited by Hervé Thiellement, Michel Zivy, Catherine Damerval, Valérie Méchin |
| title_short | Plant Proteomics |
| title_sort | plant proteomics methods and protocols |
| title_sub | Methods and Protocols |
| topic | Life Sciences Plant Sciences Life sciences Plant science Botany Proteom (DE-588)4576155-3 gnd Genetik (DE-588)4071711-2 gnd Proteomanalyse (DE-588)4596545-6 gnd Pflanzen (DE-588)4045539-7 gnd |
| topic_facet | Life Sciences Plant Sciences Life sciences Plant science Botany Proteom Genetik Proteomanalyse Pflanzen Aufsatzsammlung |
| url | https://doi.org/10.1385/1597452270 |
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