Serial Analysis of Gene Expression (SAGE): Methods and Protocols
Serial Analysis of Gene Expression (SAGE): Digital Gene Expression Profiling facilitates the introduction of SAGE into the laboratory, and provides a framework for interpreting and comparing data derived from SAGE experiments. Of the several methods of genetic profiling available, only SAGE measures...
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| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
Totowa, NJ
Humana Press
2008
|
| Schriftenreihe: | Methods in Molecular Biology™
387 |
| Schlagworte: | |
| Online-Zugang: | UBR01 TUM01 URL des Erstveröffentlichers |
| Zusammenfassung: | Serial Analysis of Gene Expression (SAGE): Digital Gene Expression Profiling facilitates the introduction of SAGE into the laboratory, and provides a framework for interpreting and comparing data derived from SAGE experiments. Of the several methods of genetic profiling available, only SAGE measures the expression of both known and unknown genes. SAGE studies encompass 50,000 tags and can provide detailed knowledge of the 2000 most highly expressed genes in the tissue sample. The SAGE protocols presented are detailed, fully annotated, and tested, and are all written by experienced SAGE researchers from around the world. Part 1 is dedicated to experimental procedures of SAGE and related methods including aRNA LongSAGE, SuperSAGE, DeepSAGE, and GMAT. Part 2 provides methods for extraction and filtration of tags, analysis of ditag populations, and completing statistically correct comparisons of gene expression profiles. Comparative transcriptomics enables scientists to understand the underlying genetics of biological changes such as development, disease, crop yield, and resistance. SAGE analysis is also used to obtain unknown tags, which can be used as gene-specific primers in Rapid Amplification of cDNA Ends (RACE) reactions to generate full-length transcripts for cloning and sequencing. This book will be an indispensable tool for any lab engaged in genetic profiling and comparative transcriptomics, and will help many laboratories to successfully implement tag-based sequencing methods and procedures and obtain comprehensive, useful, and interpretative data |
| Beschreibung: | 1 Online-Ressource (X, 208 p. 42 illus) |
| ISBN: | 9781597454544 |
| DOI: | 10.1007/978-1-59745-454-4 |
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| spelling | Serial Analysis of Gene Expression (SAGE) Methods and Protocols edited by Kåre Lehmann Nielsen Totowa, NJ Humana Press 2008 1 Online-Ressource (X, 208 p. 42 illus) txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology™ 387 Serial Analysis of Gene Expression (SAGE): Digital Gene Expression Profiling facilitates the introduction of SAGE into the laboratory, and provides a framework for interpreting and comparing data derived from SAGE experiments. Of the several methods of genetic profiling available, only SAGE measures the expression of both known and unknown genes. SAGE studies encompass 50,000 tags and can provide detailed knowledge of the 2000 most highly expressed genes in the tissue sample. The SAGE protocols presented are detailed, fully annotated, and tested, and are all written by experienced SAGE researchers from around the world. Part 1 is dedicated to experimental procedures of SAGE and related methods including aRNA LongSAGE, SuperSAGE, DeepSAGE, and GMAT. Part 2 provides methods for extraction and filtration of tags, analysis of ditag populations, and completing statistically correct comparisons of gene expression profiles. Comparative transcriptomics enables scientists to understand the underlying genetics of biological changes such as development, disease, crop yield, and resistance. SAGE analysis is also used to obtain unknown tags, which can be used as gene-specific primers in Rapid Amplification of cDNA Ends (RACE) reactions to generate full-length transcripts for cloning and sequencing. This book will be an indispensable tool for any lab engaged in genetic profiling and comparative transcriptomics, and will help many laboratories to successfully implement tag-based sequencing methods and procedures and obtain comprehensive, useful, and interpretative data Biomedicine Human Genetics Cell Biology Biochemistry, general Medicine Human genetics Biochemistry Cell biology SAGE Biochemie (DE-588)4815648-6 gnd rswk-swf 1\p (DE-588)4143413-4 Aufsatzsammlung gnd-content SAGE Biochemie (DE-588)4815648-6 s 2\p DE-604 Nielsen, Kåre Lehmann edt Erscheint auch als Druck-Ausgabe 9781588296764 https://doi.org/10.1007/978-1-59745-454-4 Verlag URL des Erstveröffentlichers Volltext 1\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk 2\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk |
| spellingShingle | Serial Analysis of Gene Expression (SAGE) Methods and Protocols Biomedicine Human Genetics Cell Biology Biochemistry, general Medicine Human genetics Biochemistry Cell biology SAGE Biochemie (DE-588)4815648-6 gnd |
| subject_GND | (DE-588)4815648-6 (DE-588)4143413-4 |
| title | Serial Analysis of Gene Expression (SAGE) Methods and Protocols |
| title_auth | Serial Analysis of Gene Expression (SAGE) Methods and Protocols |
| title_exact_search | Serial Analysis of Gene Expression (SAGE) Methods and Protocols |
| title_full | Serial Analysis of Gene Expression (SAGE) Methods and Protocols edited by Kåre Lehmann Nielsen |
| title_fullStr | Serial Analysis of Gene Expression (SAGE) Methods and Protocols edited by Kåre Lehmann Nielsen |
| title_full_unstemmed | Serial Analysis of Gene Expression (SAGE) Methods and Protocols edited by Kåre Lehmann Nielsen |
| title_short | Serial Analysis of Gene Expression (SAGE) |
| title_sort | serial analysis of gene expression sage methods and protocols |
| title_sub | Methods and Protocols |
| topic | Biomedicine Human Genetics Cell Biology Biochemistry, general Medicine Human genetics Biochemistry Cell biology SAGE Biochemie (DE-588)4815648-6 gnd |
| topic_facet | Biomedicine Human Genetics Cell Biology Biochemistry, general Medicine Human genetics Biochemistry Cell biology SAGE Biochemie Aufsatzsammlung |
| url | https://doi.org/10.1007/978-1-59745-454-4 |
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