G Protein Signaling: Methods and Protocols
G proteins and G protein-coupled receptors are ubiquitously expressed proteins that regulate a wide range of physiological processes and are consequently the targets of many pharmaceuticals. In G Protein Signaling: Methods and Protocols, leading researchers describe in detail key methods for investi...
Gespeichert in:
| Weitere Verfasser: | |
|---|---|
| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
Totowa, NJ
Humana Press
2004
|
| Schriftenreihe: | Methods in Molecular Biology™
237 |
| Schlagworte: | |
| Online-Zugang: | UBR01 TUM01 Volltext |
| Zusammenfassung: | G proteins and G protein-coupled receptors are ubiquitously expressed proteins that regulate a wide range of physiological processes and are consequently the targets of many pharmaceuticals. In G Protein Signaling: Methods and Protocols, leading researchers describe in detail key methods for investigating G protein signaling from a variety of perspectives ranging from in vitro biochemistry to whole animal studies. The authors focus on the mechanisms of G protein and G protein-coupled receptor function and the roles of G protein subunits in cell biology and disease. Among the readily reproducible techniques presented are those for the purification of G proteins and effector enzymes, assays of these purified G proteins and effector enzymes, and for the study of G protein interactions with effectors in intact cells. Additional methods are provided for assaying G protein-coupled receptor structure, function, and localization, for studying the physiological roles for endogenous G proteins, and for examining lipid and phosphate modifications of RGS proteins. Each fully tested protocol includes a background introduction explaining the principle behind the technique, equipment and reagent lists, tips on troubleshooting and avoiding known pitfalls, and, where needed, a discussion of the interpretation and use of results. Comprehensive and highly practical, G Protein Signaling: Methods and Protocols offers novice and expert researchers alike an array of cutting-edge tools to illuminate the roles of G protein and G protein-coupled receptors, as well as a better understanding of their action with drugs, neurotransmitters, and sensory stimuli |
| Beschreibung: | 1 Online-Ressource (XII, 250 p) |
| ISBN: | 9781592594306 |
| DOI: | 10.1385/1592594301 |
Internformat
MARC
| LEADER | 00000nmm a2200000zcb4500 | ||
|---|---|---|---|
| 001 | BV044951325 | ||
| 003 | DE-604 | ||
| 005 | 20180925 | ||
| 007 | cr|uuu---uuuuu | ||
| 008 | 180517s2004 |||| o||u| ||||||eng d | ||
| 020 | |a 9781592594306 |9 978-1-59259-430-6 | ||
| 024 | 7 | |a 10.1385/1592594301 |2 doi | |
| 035 | |a (ZDB-2-PRO)978-1-59259-430-6 | ||
| 035 | |a (OCoLC)730584226 | ||
| 035 | |a (DE-599)BVBBV044951325 | ||
| 040 | |a DE-604 |b ger |e rda | ||
| 041 | 0 | |a eng | |
| 049 | |a DE-355 |a DE-91 | ||
| 082 | 0 | |a 571.6 |2 23 | |
| 084 | |a WC 4350 |0 (DE-625)148095: |2 rvk | ||
| 084 | |a WE 5320 |0 (DE-625)148305: |2 rvk | ||
| 245 | 1 | 0 | |a G Protein Signaling |b Methods and Protocols |c edited by Alan V. Smrcka |
| 264 | 1 | |a Totowa, NJ |b Humana Press |c 2004 | |
| 300 | |a 1 Online-Ressource (XII, 250 p) | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b c |2 rdamedia | ||
| 338 | |b cr |2 rdacarrier | ||
| 490 | 0 | |a Methods in Molecular Biology™ |v 237 | |
| 520 | |a G proteins and G protein-coupled receptors are ubiquitously expressed proteins that regulate a wide range of physiological processes and are consequently the targets of many pharmaceuticals. In G Protein Signaling: Methods and Protocols, leading researchers describe in detail key methods for investigating G protein signaling from a variety of perspectives ranging from in vitro biochemistry to whole animal studies. The authors focus on the mechanisms of G protein and G protein-coupled receptor function and the roles of G protein subunits in cell biology and disease. Among the readily reproducible techniques presented are those for the purification of G proteins and effector enzymes, assays of these purified G proteins and effector enzymes, and for the study of G protein interactions with effectors in intact cells. Additional methods are provided for assaying G protein-coupled receptor structure, function, and localization, for studying the physiological roles for endogenous G proteins, and for examining lipid and phosphate modifications of RGS proteins. Each fully tested protocol includes a background introduction explaining the principle behind the technique, equipment and reagent lists, tips on troubleshooting and avoiding known pitfalls, and, where needed, a discussion of the interpretation and use of results. Comprehensive and highly practical, G Protein Signaling: Methods and Protocols offers novice and expert researchers alike an array of cutting-edge tools to illuminate the roles of G protein and G protein-coupled receptors, as well as a better understanding of their action with drugs, neurotransmitters, and sensory stimuli | ||
| 650 | 4 | |a Life Sciences | |
| 650 | 4 | |a Cell Biology | |
| 650 | 4 | |a Life sciences | |
| 650 | 4 | |a Cell biology | |
| 650 | 0 | 7 | |a Untersuchungsmethode |0 (DE-588)4324907-3 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a GTP-bindende Proteine |0 (DE-588)4248354-2 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Signaltransduktion |0 (DE-588)4318717-1 |2 gnd |9 rswk-swf |
| 655 | 7 | |8 1\p |0 (DE-588)4143413-4 |a Aufsatzsammlung |2 gnd-content | |
| 689 | 0 | 0 | |a GTP-bindende Proteine |0 (DE-588)4248354-2 |D s |
| 689 | 0 | 1 | |a Signaltransduktion |0 (DE-588)4318717-1 |D s |
| 689 | 0 | 2 | |a Untersuchungsmethode |0 (DE-588)4324907-3 |D s |
| 689 | 0 | |5 DE-604 | |
| 700 | 1 | |a Smrcka, Alan V. |4 edt | |
| 776 | 0 | 8 | |i Erscheint auch als |n Druck-Ausgabe |z 9781588291370 |
| 856 | 4 | 0 | |u https://doi.org/10.1385/1592594301 |x Verlag |z URL des Erstveröffentlichers |3 Volltext |
| 912 | |a ZDB-2-PRO | ||
| 999 | |a oai:aleph.bib-bvb.de:BVB01-030344082 | ||
| 883 | 1 | |8 1\p |a cgwrk |d 20201028 |q DE-101 |u https://d-nb.info/provenance/plan#cgwrk | |
| 966 | e | |u https://doi.org/10.1385/1592594301 |l UBR01 |p ZDB-2-PRO |x Verlag |3 Volltext | |
| 966 | e | |u https://doi.org/10.1385/1592594301 |l TUM01 |p ZDB-2-PRO |x Verlag |3 Volltext | |
Datensatz im Suchindex
| _version_ | 1804178539807768576 |
|---|---|
| any_adam_object | |
| author2 | Smrcka, Alan V. |
| author2_role | edt |
| author2_variant | a v s av avs |
| author_facet | Smrcka, Alan V. |
| building | Verbundindex |
| bvnumber | BV044951325 |
| classification_rvk | WC 4350 WE 5320 |
| collection | ZDB-2-PRO |
| ctrlnum | (ZDB-2-PRO)978-1-59259-430-6 (OCoLC)730584226 (DE-599)BVBBV044951325 |
| dewey-full | 571.6 |
| dewey-hundreds | 500 - Natural sciences and mathematics |
| dewey-ones | 571 - Physiology & related subjects |
| dewey-raw | 571.6 |
| dewey-search | 571.6 |
| dewey-sort | 3571.6 |
| dewey-tens | 570 - Biology |
| discipline | Biologie |
| doi_str_mv | 10.1385/1592594301 |
| format | Electronic eBook |
| fullrecord | <?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>03722nmm a2200553zcb4500</leader><controlfield tag="001">BV044951325</controlfield><controlfield tag="003">DE-604</controlfield><controlfield tag="005">20180925 </controlfield><controlfield tag="007">cr|uuu---uuuuu</controlfield><controlfield tag="008">180517s2004 |||| o||u| ||||||eng d</controlfield><datafield tag="020" ind1=" " ind2=" "><subfield code="a">9781592594306</subfield><subfield code="9">978-1-59259-430-6</subfield></datafield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1385/1592594301</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(ZDB-2-PRO)978-1-59259-430-6</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(OCoLC)730584226</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)BVBBV044951325</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-604</subfield><subfield code="b">ger</subfield><subfield code="e">rda</subfield></datafield><datafield tag="041" ind1="0" ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="049" ind1=" " ind2=" "><subfield code="a">DE-355</subfield><subfield code="a">DE-91</subfield></datafield><datafield tag="082" ind1="0" ind2=" "><subfield code="a">571.6</subfield><subfield code="2">23</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">WC 4350</subfield><subfield code="0">(DE-625)148095:</subfield><subfield code="2">rvk</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">WE 5320</subfield><subfield code="0">(DE-625)148305:</subfield><subfield code="2">rvk</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">G Protein Signaling</subfield><subfield code="b">Methods and Protocols</subfield><subfield code="c">edited by Alan V. Smrcka</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="a">Totowa, NJ</subfield><subfield code="b">Humana Press</subfield><subfield code="c">2004</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">1 Online-Ressource (XII, 250 p)</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="490" ind1="0" ind2=" "><subfield code="a">Methods in Molecular Biology™</subfield><subfield code="v">237</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">G proteins and G protein-coupled receptors are ubiquitously expressed proteins that regulate a wide range of physiological processes and are consequently the targets of many pharmaceuticals. In G Protein Signaling: Methods and Protocols, leading researchers describe in detail key methods for investigating G protein signaling from a variety of perspectives ranging from in vitro biochemistry to whole animal studies. The authors focus on the mechanisms of G protein and G protein-coupled receptor function and the roles of G protein subunits in cell biology and disease. Among the readily reproducible techniques presented are those for the purification of G proteins and effector enzymes, assays of these purified G proteins and effector enzymes, and for the study of G protein interactions with effectors in intact cells. Additional methods are provided for assaying G protein-coupled receptor structure, function, and localization, for studying the physiological roles for endogenous G proteins, and for examining lipid and phosphate modifications of RGS proteins. Each fully tested protocol includes a background introduction explaining the principle behind the technique, equipment and reagent lists, tips on troubleshooting and avoiding known pitfalls, and, where needed, a discussion of the interpretation and use of results. Comprehensive and highly practical, G Protein Signaling: Methods and Protocols offers novice and expert researchers alike an array of cutting-edge tools to illuminate the roles of G protein and G protein-coupled receptors, as well as a better understanding of their action with drugs, neurotransmitters, and sensory stimuli</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Life Sciences</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Cell Biology</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Life sciences</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Cell biology</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Untersuchungsmethode</subfield><subfield code="0">(DE-588)4324907-3</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">GTP-bindende Proteine</subfield><subfield code="0">(DE-588)4248354-2</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Signaltransduktion</subfield><subfield code="0">(DE-588)4318717-1</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="655" ind1=" " ind2="7"><subfield code="8">1\p</subfield><subfield code="0">(DE-588)4143413-4</subfield><subfield code="a">Aufsatzsammlung</subfield><subfield code="2">gnd-content</subfield></datafield><datafield tag="689" ind1="0" ind2="0"><subfield code="a">GTP-bindende Proteine</subfield><subfield code="0">(DE-588)4248354-2</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2="1"><subfield code="a">Signaltransduktion</subfield><subfield code="0">(DE-588)4318717-1</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2="2"><subfield code="a">Untersuchungsmethode</subfield><subfield code="0">(DE-588)4324907-3</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2=" "><subfield code="5">DE-604</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Smrcka, Alan V.</subfield><subfield code="4">edt</subfield></datafield><datafield tag="776" ind1="0" ind2="8"><subfield code="i">Erscheint auch als</subfield><subfield code="n">Druck-Ausgabe</subfield><subfield code="z">9781588291370</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://doi.org/10.1385/1592594301</subfield><subfield code="x">Verlag</subfield><subfield code="z">URL des Erstveröffentlichers</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">ZDB-2-PRO</subfield></datafield><datafield tag="999" ind1=" " ind2=" "><subfield code="a">oai:aleph.bib-bvb.de:BVB01-030344082</subfield></datafield><datafield tag="883" ind1="1" ind2=" "><subfield code="8">1\p</subfield><subfield code="a">cgwrk</subfield><subfield code="d">20201028</subfield><subfield code="q">DE-101</subfield><subfield code="u">https://d-nb.info/provenance/plan#cgwrk</subfield></datafield><datafield tag="966" ind1="e" ind2=" "><subfield code="u">https://doi.org/10.1385/1592594301</subfield><subfield code="l">UBR01</subfield><subfield code="p">ZDB-2-PRO</subfield><subfield code="x">Verlag</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="966" ind1="e" ind2=" "><subfield code="u">https://doi.org/10.1385/1592594301</subfield><subfield code="l">TUM01</subfield><subfield code="p">ZDB-2-PRO</subfield><subfield code="x">Verlag</subfield><subfield code="3">Volltext</subfield></datafield></record></collection> |
| genre | 1\p (DE-588)4143413-4 Aufsatzsammlung gnd-content |
| genre_facet | Aufsatzsammlung |
| id | DE-604.BV044951325 |
| illustrated | Not Illustrated |
| indexdate | 2024-07-10T08:05:35Z |
| institution | BVB |
| isbn | 9781592594306 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-030344082 |
| oclc_num | 730584226 |
| open_access_boolean | |
| owner | DE-355 DE-BY-UBR DE-91 DE-BY-TUM |
| owner_facet | DE-355 DE-BY-UBR DE-91 DE-BY-TUM |
| physical | 1 Online-Ressource (XII, 250 p) |
| psigel | ZDB-2-PRO |
| publishDate | 2004 |
| publishDateSearch | 2004 |
| publishDateSort | 2004 |
| publisher | Humana Press |
| record_format | marc |
| series2 | Methods in Molecular Biology™ |
| spelling | G Protein Signaling Methods and Protocols edited by Alan V. Smrcka Totowa, NJ Humana Press 2004 1 Online-Ressource (XII, 250 p) txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology™ 237 G proteins and G protein-coupled receptors are ubiquitously expressed proteins that regulate a wide range of physiological processes and are consequently the targets of many pharmaceuticals. In G Protein Signaling: Methods and Protocols, leading researchers describe in detail key methods for investigating G protein signaling from a variety of perspectives ranging from in vitro biochemistry to whole animal studies. The authors focus on the mechanisms of G protein and G protein-coupled receptor function and the roles of G protein subunits in cell biology and disease. Among the readily reproducible techniques presented are those for the purification of G proteins and effector enzymes, assays of these purified G proteins and effector enzymes, and for the study of G protein interactions with effectors in intact cells. Additional methods are provided for assaying G protein-coupled receptor structure, function, and localization, for studying the physiological roles for endogenous G proteins, and for examining lipid and phosphate modifications of RGS proteins. Each fully tested protocol includes a background introduction explaining the principle behind the technique, equipment and reagent lists, tips on troubleshooting and avoiding known pitfalls, and, where needed, a discussion of the interpretation and use of results. Comprehensive and highly practical, G Protein Signaling: Methods and Protocols offers novice and expert researchers alike an array of cutting-edge tools to illuminate the roles of G protein and G protein-coupled receptors, as well as a better understanding of their action with drugs, neurotransmitters, and sensory stimuli Life Sciences Cell Biology Life sciences Cell biology Untersuchungsmethode (DE-588)4324907-3 gnd rswk-swf GTP-bindende Proteine (DE-588)4248354-2 gnd rswk-swf Signaltransduktion (DE-588)4318717-1 gnd rswk-swf 1\p (DE-588)4143413-4 Aufsatzsammlung gnd-content GTP-bindende Proteine (DE-588)4248354-2 s Signaltransduktion (DE-588)4318717-1 s Untersuchungsmethode (DE-588)4324907-3 s DE-604 Smrcka, Alan V. edt Erscheint auch als Druck-Ausgabe 9781588291370 https://doi.org/10.1385/1592594301 Verlag URL des Erstveröffentlichers Volltext 1\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk |
| spellingShingle | G Protein Signaling Methods and Protocols Life Sciences Cell Biology Life sciences Cell biology Untersuchungsmethode (DE-588)4324907-3 gnd GTP-bindende Proteine (DE-588)4248354-2 gnd Signaltransduktion (DE-588)4318717-1 gnd |
| subject_GND | (DE-588)4324907-3 (DE-588)4248354-2 (DE-588)4318717-1 (DE-588)4143413-4 |
| title | G Protein Signaling Methods and Protocols |
| title_auth | G Protein Signaling Methods and Protocols |
| title_exact_search | G Protein Signaling Methods and Protocols |
| title_full | G Protein Signaling Methods and Protocols edited by Alan V. Smrcka |
| title_fullStr | G Protein Signaling Methods and Protocols edited by Alan V. Smrcka |
| title_full_unstemmed | G Protein Signaling Methods and Protocols edited by Alan V. Smrcka |
| title_short | G Protein Signaling |
| title_sort | g protein signaling methods and protocols |
| title_sub | Methods and Protocols |
| topic | Life Sciences Cell Biology Life sciences Cell biology Untersuchungsmethode (DE-588)4324907-3 gnd GTP-bindende Proteine (DE-588)4248354-2 gnd Signaltransduktion (DE-588)4318717-1 gnd |
| topic_facet | Life Sciences Cell Biology Life sciences Cell biology Untersuchungsmethode GTP-bindende Proteine Signaltransduktion Aufsatzsammlung |
| url | https://doi.org/10.1385/1592594301 |
| work_keys_str_mv | AT smrckaalanv gproteinsignalingmethodsandprotocols |