RT-PCR Protocols: Second Edition
Once a tedious, highly skilled operation, reverse-transcription polymerase chain reaction (RT-PCR) has become a routine and invaluable technique used in most laboratories. In RT-PCR Protocols, Second Edition, expert researchers fully update the technologies presented in the popular previous edition,...
Gespeichert in:
| Weitere Verfasser: | |
|---|---|
| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
Totowa, NJ
Humana Press
2010
|
| Ausgabe: | Second Edition |
| Schriftenreihe: | Methods in Molecular Biology, Methods and Protocols
630 |
| Schlagworte: | |
| Online-Zugang: | UBR01 TUM01 Volltext |
| Zusammenfassung: | Once a tedious, highly skilled operation, reverse-transcription polymerase chain reaction (RT-PCR) has become a routine and invaluable technique used in most laboratories. In RT-PCR Protocols, Second Edition, expert researchers fully update the technologies presented in the popular previous edition, such as competitive RT-PCR, nested RT-PCR, RT-PCR from single cells, and RT-PCR for cloning. In addition, newer technologies are also explored, including multiplex RT-PCR, RT-LATE-PCR, and the greatly advanced field of real-time quantitative RT-PCR, while recent advances in creating the optimum RT-PCR reaction, e.g. RNA extraction, primer design, and reverse transcription, end the book with their indispensable input. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes sections, highlighting tips on troubleshooting and avoiding known pitfalls. User friendly and up-to-date, RT-PCR Protocols, Second Edition acts as a handy companion to scientists from numerous diverse backgrounds who wish to explore further the marvels of gene expression |
| Beschreibung: | 1 Online-Ressource (XI, 341 p. 65 illus., 4 illus. in color) |
| ISBN: | 9781607616290 |
| DOI: | 10.1007/978-1-60761-629-0 |
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| 520 | |a Once a tedious, highly skilled operation, reverse-transcription polymerase chain reaction (RT-PCR) has become a routine and invaluable technique used in most laboratories. In RT-PCR Protocols, Second Edition, expert researchers fully update the technologies presented in the popular previous edition, such as competitive RT-PCR, nested RT-PCR, RT-PCR from single cells, and RT-PCR for cloning. In addition, newer technologies are also explored, including multiplex RT-PCR, RT-LATE-PCR, and the greatly advanced field of real-time quantitative RT-PCR, while recent advances in creating the optimum RT-PCR reaction, e.g. RNA extraction, primer design, and reverse transcription, end the book with their indispensable input. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes sections, highlighting tips on troubleshooting and avoiding known pitfalls. User friendly and up-to-date, RT-PCR Protocols, Second Edition acts as a handy companion to scientists from numerous diverse backgrounds who wish to explore further the marvels of gene expression | ||
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| edition | Second Edition |
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| spelling | RT-PCR Protocols Second Edition edited by Nicola King Second Edition Totowa, NJ Humana Press 2010 1 Online-Ressource (XI, 341 p. 65 illus., 4 illus. in color) txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology, Methods and Protocols 630 Once a tedious, highly skilled operation, reverse-transcription polymerase chain reaction (RT-PCR) has become a routine and invaluable technique used in most laboratories. In RT-PCR Protocols, Second Edition, expert researchers fully update the technologies presented in the popular previous edition, such as competitive RT-PCR, nested RT-PCR, RT-PCR from single cells, and RT-PCR for cloning. In addition, newer technologies are also explored, including multiplex RT-PCR, RT-LATE-PCR, and the greatly advanced field of real-time quantitative RT-PCR, while recent advances in creating the optimum RT-PCR reaction, e.g. RNA extraction, primer design, and reverse transcription, end the book with their indispensable input. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes sections, highlighting tips on troubleshooting and avoiding known pitfalls. User friendly and up-to-date, RT-PCR Protocols, Second Edition acts as a handy companion to scientists from numerous diverse backgrounds who wish to explore further the marvels of gene expression Biomedicine Human Genetics Gene Expression Medicine Human genetics Gene expression Reverse Transkriptase-Polymerase-Kettenreaktion (DE-588)7544647-9 gnd rswk-swf Methode (DE-588)4038971-6 gnd rswk-swf 1\p (DE-588)4143413-4 Aufsatzsammlung gnd-content Reverse Transkriptase-Polymerase-Kettenreaktion (DE-588)7544647-9 s Methode (DE-588)4038971-6 s DE-604 King, Nicola edt Erscheint auch als Druck-Ausgabe 9781607616283 https://doi.org/10.1007/978-1-60761-629-0 Verlag URL des Erstveröffentlichers Volltext 1\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk |
| spellingShingle | RT-PCR Protocols Second Edition Biomedicine Human Genetics Gene Expression Medicine Human genetics Gene expression Reverse Transkriptase-Polymerase-Kettenreaktion (DE-588)7544647-9 gnd Methode (DE-588)4038971-6 gnd |
| subject_GND | (DE-588)7544647-9 (DE-588)4038971-6 (DE-588)4143413-4 |
| title | RT-PCR Protocols Second Edition |
| title_auth | RT-PCR Protocols Second Edition |
| title_exact_search | RT-PCR Protocols Second Edition |
| title_full | RT-PCR Protocols Second Edition edited by Nicola King |
| title_fullStr | RT-PCR Protocols Second Edition edited by Nicola King |
| title_full_unstemmed | RT-PCR Protocols Second Edition edited by Nicola King |
| title_short | RT-PCR Protocols |
| title_sort | rt pcr protocols second edition |
| title_sub | Second Edition |
| topic | Biomedicine Human Genetics Gene Expression Medicine Human genetics Gene expression Reverse Transkriptase-Polymerase-Kettenreaktion (DE-588)7544647-9 gnd Methode (DE-588)4038971-6 gnd |
| topic_facet | Biomedicine Human Genetics Gene Expression Medicine Human genetics Gene expression Reverse Transkriptase-Polymerase-Kettenreaktion Methode Aufsatzsammlung |
| url | https://doi.org/10.1007/978-1-60761-629-0 |
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