Receptor Signal Transduction Protocols:
In this second edition of a widely appreciated work, Receptor Signal Transduction Protocols, a panel of internationally recognized investigators presents their best methods for studying G-protein-coupled receptors (GPCRs) and events immediately downstream of their activation. This new edition combin...
Gespeichert in:
| Weitere Verfasser: | , |
|---|---|
| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
Totowa, NJ
Humana Press
2004
|
| Ausgabe: | Second Edition |
| Schriftenreihe: | Methods in Molecular Biology
259 |
| Schlagworte: | |
| Online-Zugang: | UBR01 TUM01 Volltext |
| Zusammenfassung: | In this second edition of a widely appreciated work, Receptor Signal Transduction Protocols, a panel of internationally recognized investigators presents their best methods for studying G-protein-coupled receptors (GPCRs) and events immediately downstream of their activation. This new edition combines updates of key chapters from the first edition with a large number of new contributions on the many successful methodologies that have emerged more recently. The methods are focused primarily on events at the receptor level, including ligand binding, on the genetic manipulation of receptors, the generation of model cell lines in which to study them, and the interaction and activation of G-proteins. Additional methods concentrate on receptor expression and localization, receptor internalization and post-translational modification, GPCR-protein interactions, and the use of knock-out and knock-in strategies for determining the physiological roles of receptors. The laboratory protocols follow the successful Methods in Molecular Biology™ series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and highly practical, Receptor Signal Transduction Protocols, Second Edition offers both novice and experienced investigators powerful cutting-edge techniques that provide an array of approaches and specific methods that will aid in the understanding of GPCR structure and function |
| Beschreibung: | 1 Online-Ressource (XIII, 418 p) |
| ISBN: | 9781592597543 |
| DOI: | 10.1385/1592597548 |
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| 520 | |a In this second edition of a widely appreciated work, Receptor Signal Transduction Protocols, a panel of internationally recognized investigators presents their best methods for studying G-protein-coupled receptors (GPCRs) and events immediately downstream of their activation. This new edition combines updates of key chapters from the first edition with a large number of new contributions on the many successful methodologies that have emerged more recently. The methods are focused primarily on events at the receptor level, including ligand binding, on the genetic manipulation of receptors, the generation of model cell lines in which to study them, and the interaction and activation of G-proteins. Additional methods concentrate on receptor expression and localization, receptor internalization and post-translational modification, GPCR-protein interactions, and the use of knock-out and knock-in strategies for determining the physiological roles of receptors. The laboratory protocols follow the successful Methods in Molecular Biology™ series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and highly practical, Receptor Signal Transduction Protocols, Second Edition offers both novice and experienced investigators powerful cutting-edge techniques that provide an array of approaches and specific methods that will aid in the understanding of GPCR structure and function | ||
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| spelling | Receptor Signal Transduction Protocols edited by Gary B. Willars, R. A. John Challiss Second Edition Totowa, NJ Humana Press 2004 1 Online-Ressource (XIII, 418 p) txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology 259 In this second edition of a widely appreciated work, Receptor Signal Transduction Protocols, a panel of internationally recognized investigators presents their best methods for studying G-protein-coupled receptors (GPCRs) and events immediately downstream of their activation. This new edition combines updates of key chapters from the first edition with a large number of new contributions on the many successful methodologies that have emerged more recently. The methods are focused primarily on events at the receptor level, including ligand binding, on the genetic manipulation of receptors, the generation of model cell lines in which to study them, and the interaction and activation of G-proteins. Additional methods concentrate on receptor expression and localization, receptor internalization and post-translational modification, GPCR-protein interactions, and the use of knock-out and knock-in strategies for determining the physiological roles of receptors. The laboratory protocols follow the successful Methods in Molecular Biology™ series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and highly practical, Receptor Signal Transduction Protocols, Second Edition offers both novice and experienced investigators powerful cutting-edge techniques that provide an array of approaches and specific methods that will aid in the understanding of GPCR structure and function Life Sciences Cell Biology Life sciences Cell biology Labortechnik (DE-588)4123602-6 gnd rswk-swf Zellrezeptor (DE-588)4133853-4 gnd rswk-swf Rezeptor (DE-588)4049722-7 gnd rswk-swf Signaltransduktion (DE-588)4318717-1 gnd rswk-swf Methode (DE-588)4038971-6 gnd rswk-swf 1\p (DE-588)4143413-4 Aufsatzsammlung gnd-content Rezeptor (DE-588)4049722-7 s Signaltransduktion (DE-588)4318717-1 s Labortechnik (DE-588)4123602-6 s DE-604 Zellrezeptor (DE-588)4133853-4 s Methode (DE-588)4038971-6 s 2\p DE-604 3\p DE-604 Willars, Gary B. edt Challiss, R. A. John edt Erscheint auch als Druck-Ausgabe 9781588293299 https://doi.org/10.1385/1592597548 Verlag URL des Erstveröffentlichers Volltext 1\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk 2\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk 3\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk |
| spellingShingle | Receptor Signal Transduction Protocols Life Sciences Cell Biology Life sciences Cell biology Labortechnik (DE-588)4123602-6 gnd Zellrezeptor (DE-588)4133853-4 gnd Rezeptor (DE-588)4049722-7 gnd Signaltransduktion (DE-588)4318717-1 gnd Methode (DE-588)4038971-6 gnd |
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| title | Receptor Signal Transduction Protocols |
| title_auth | Receptor Signal Transduction Protocols |
| title_exact_search | Receptor Signal Transduction Protocols |
| title_full | Receptor Signal Transduction Protocols edited by Gary B. Willars, R. A. John Challiss |
| title_fullStr | Receptor Signal Transduction Protocols edited by Gary B. Willars, R. A. John Challiss |
| title_full_unstemmed | Receptor Signal Transduction Protocols edited by Gary B. Willars, R. A. John Challiss |
| title_short | Receptor Signal Transduction Protocols |
| title_sort | receptor signal transduction protocols |
| topic | Life Sciences Cell Biology Life sciences Cell biology Labortechnik (DE-588)4123602-6 gnd Zellrezeptor (DE-588)4133853-4 gnd Rezeptor (DE-588)4049722-7 gnd Signaltransduktion (DE-588)4318717-1 gnd Methode (DE-588)4038971-6 gnd |
| topic_facet | Life Sciences Cell Biology Life sciences Cell biology Labortechnik Zellrezeptor Rezeptor Signaltransduktion Methode Aufsatzsammlung |
| url | https://doi.org/10.1385/1592597548 |
| work_keys_str_mv | AT willarsgaryb receptorsignaltransductionprotocols AT challissrajohn receptorsignaltransductionprotocols |