Neurotrophin Protocols:
Despite the extraordinary growth of research interest in neurotrophic factors, the techniques available have often been inadequate or just emergent from other disciplines. In Neurotrophin Protocols, established leaders in the neurotrophin field detail their special expertise in a wide variety of key...
Gespeichert in:
| Weitere Verfasser: | |
|---|---|
| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
Totowa, NJ
Humana Press
2001
|
| Schriftenreihe: | Methods in Molecular Biology™
169 |
| Schlagworte: | |
| Online-Zugang: | UBR01 TUM01 Volltext |
| Zusammenfassung: | Despite the extraordinary growth of research interest in neurotrophic factors, the techniques available have often been inadequate or just emergent from other disciplines. In Neurotrophin Protocols, established leaders in the neurotrophin field detail their special expertise in a wide variety of key protein, RNA, recombinant, and in vivo techniques. The protocols range from immunological analysis for the cellular localization and quantification of the neurotrophins, to genetic manipulation of cells and animals for the analysis of biological function, to quantitative analysis of the active neurotrophin genes. There are also radiotracing techniques for studying neurotrophin transport in both the retrograde and anterograde directions, procedures for using immunotoxins to study the effects of eliminating a single class of neurons, and the essential stereological method for estimation of neuronal numbers. Each method includes not only detailed step-by-step instructions, but also a list of necessary equipment and supplies, and valuable notes spelling out quick tips and tricks of the trade. Timely and robust, Neurotrophin Protocols provides today's neuroscientists in both academia and industry with a comprehensive range of practical, readily reproducible methods for studying neurotrophins and the critically important effects they have on the nervous system |
| Beschreibung: | 1 Online-Ressource (X, 274 p. 41 illus) |
| ISBN: | 9781592590605 |
| DOI: | 10.1385/1592590608 |
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| 520 | |a Despite the extraordinary growth of research interest in neurotrophic factors, the techniques available have often been inadequate or just emergent from other disciplines. In Neurotrophin Protocols, established leaders in the neurotrophin field detail their special expertise in a wide variety of key protein, RNA, recombinant, and in vivo techniques. The protocols range from immunological analysis for the cellular localization and quantification of the neurotrophins, to genetic manipulation of cells and animals for the analysis of biological function, to quantitative analysis of the active neurotrophin genes. There are also radiotracing techniques for studying neurotrophin transport in both the retrograde and anterograde directions, procedures for using immunotoxins to study the effects of eliminating a single class of neurons, and the essential stereological method for estimation of neuronal numbers. Each method includes not only detailed step-by-step instructions, but also a list of necessary equipment and supplies, and valuable notes spelling out quick tips and tricks of the trade. Timely and robust, Neurotrophin Protocols provides today's neuroscientists in both academia and industry with a comprehensive range of practical, readily reproducible methods for studying neurotrophins and the critically important effects they have on the nervous system | ||
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Datensatz im Suchindex
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| discipline | Biologie Medizin |
| doi_str_mv | 10.1385/1592590608 |
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| spelling | Neurotrophin Protocols edited by Robert A. Rush Totowa, NJ Humana Press 2001 1 Online-Ressource (X, 274 p. 41 illus) txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology™ 169 Despite the extraordinary growth of research interest in neurotrophic factors, the techniques available have often been inadequate or just emergent from other disciplines. In Neurotrophin Protocols, established leaders in the neurotrophin field detail their special expertise in a wide variety of key protein, RNA, recombinant, and in vivo techniques. The protocols range from immunological analysis for the cellular localization and quantification of the neurotrophins, to genetic manipulation of cells and animals for the analysis of biological function, to quantitative analysis of the active neurotrophin genes. There are also radiotracing techniques for studying neurotrophin transport in both the retrograde and anterograde directions, procedures for using immunotoxins to study the effects of eliminating a single class of neurons, and the essential stereological method for estimation of neuronal numbers. Each method includes not only detailed step-by-step instructions, but also a list of necessary equipment and supplies, and valuable notes spelling out quick tips and tricks of the trade. Timely and robust, Neurotrophin Protocols provides today's neuroscientists in both academia and industry with a comprehensive range of practical, readily reproducible methods for studying neurotrophins and the critically important effects they have on the nervous system Biomedicine Neurosciences Medicine Neurotropher Faktor (DE-588)4317638-0 gnd rswk-swf Genexpression (DE-588)4020136-3 gnd rswk-swf Immuncytochemie (DE-588)4161368-5 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Neurotropher Faktor (DE-588)4317638-0 s Immuncytochemie (DE-588)4161368-5 s DE-604 Genexpression (DE-588)4020136-3 s Rush, Robert A. edt Erscheint auch als Druck-Ausgabe 9780896036994 https://doi.org/10.1385/1592590608 Verlag URL des Erstveröffentlichers Volltext |
| spellingShingle | Neurotrophin Protocols Biomedicine Neurosciences Medicine Neurotropher Faktor (DE-588)4317638-0 gnd Genexpression (DE-588)4020136-3 gnd Immuncytochemie (DE-588)4161368-5 gnd |
| subject_GND | (DE-588)4317638-0 (DE-588)4020136-3 (DE-588)4161368-5 (DE-588)4143413-4 |
| title | Neurotrophin Protocols |
| title_auth | Neurotrophin Protocols |
| title_exact_search | Neurotrophin Protocols |
| title_full | Neurotrophin Protocols edited by Robert A. Rush |
| title_fullStr | Neurotrophin Protocols edited by Robert A. Rush |
| title_full_unstemmed | Neurotrophin Protocols edited by Robert A. Rush |
| title_short | Neurotrophin Protocols |
| title_sort | neurotrophin protocols |
| topic | Biomedicine Neurosciences Medicine Neurotropher Faktor (DE-588)4317638-0 gnd Genexpression (DE-588)4020136-3 gnd Immuncytochemie (DE-588)4161368-5 gnd |
| topic_facet | Biomedicine Neurosciences Medicine Neurotropher Faktor Genexpression Immuncytochemie Aufsatzsammlung |
| url | https://doi.org/10.1385/1592590608 |
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