Protein NMR Techniques:
Carrying on in the tradition of its much praised earlier edition, A. Kristina Downing has selected new protocols describing the many recent advances in NMR methodology that are revolutionizing the field. Described in step-by-step detail by established hands-on experts, these readily reproducible tec...
Gespeichert in:
Weitere Verfasser: | |
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Format: | Elektronisch E-Book |
Sprache: | English |
Veröffentlicht: |
Totowa, NJ
Humana Press
2004
|
Ausgabe: | Second Edition |
Schriftenreihe: | Methods in Molecular Biology™
278 |
Schlagworte: | |
Online-Zugang: | UBR01 TUM01 Volltext |
Zusammenfassung: | Carrying on in the tradition of its much praised earlier edition, A. Kristina Downing has selected new protocols describing the many recent advances in NMR methodology that are revolutionizing the field. Described in step-by-step detail by established hands-on experts, these readily reproducible techniques include methods for high-level recombinant protein expression using sophisticated isotopic labeling strategies, TROSY methods for the study of structure and dynamics, and methods for the acquisition and interpretation of residual dipolar coupling data. There are also applications of dynamics measurements on multiple timescales, new developments in NMR data analysis, structure calculation protocols, and a comprehensive chapter on solid-state methods for the study of membrane proteins. The protocols follow the successful Methods in Molecular Biology™ series format, each offering detailed laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. State-of-the-art and highly practical, Protein NMR Techniques, 2nd Ed., makes accessible to all NMR laboratories proven techniques that are powerfully transforming the speed, accuracy, and size limit of our ability to characterize biological macromolecules |
Beschreibung: | 1 Online-Ressource (XIII, 487 p) |
ISBN: | 9781592598090 |
DOI: | 10.1385/1592598099 |
Internformat
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Datensatz im Suchindex
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dewey-ones | 572 - Biochemistry |
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discipline | Biologie Chemie |
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spelling | Protein NMR Techniques edited by A. Kristina Downing Second Edition Totowa, NJ Humana Press 2004 1 Online-Ressource (XIII, 487 p) txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology™ 278 Carrying on in the tradition of its much praised earlier edition, A. Kristina Downing has selected new protocols describing the many recent advances in NMR methodology that are revolutionizing the field. Described in step-by-step detail by established hands-on experts, these readily reproducible techniques include methods for high-level recombinant protein expression using sophisticated isotopic labeling strategies, TROSY methods for the study of structure and dynamics, and methods for the acquisition and interpretation of residual dipolar coupling data. There are also applications of dynamics measurements on multiple timescales, new developments in NMR data analysis, structure calculation protocols, and a comprehensive chapter on solid-state methods for the study of membrane proteins. The protocols follow the successful Methods in Molecular Biology™ series format, each offering detailed laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. State-of-the-art and highly practical, Protein NMR Techniques, 2nd Ed., makes accessible to all NMR laboratories proven techniques that are powerfully transforming the speed, accuracy, and size limit of our ability to characterize biological macromolecules Life Sciences Biochemistry, general Life sciences Biochemistry Labortechnik (DE-588)4123602-6 gnd rswk-swf NMR-Spektroskopie (DE-588)4075421-2 gnd rswk-swf Proteine (DE-588)4076388-2 gnd rswk-swf 1\p (DE-588)4143413-4 Aufsatzsammlung gnd-content Proteine (DE-588)4076388-2 s NMR-Spektroskopie (DE-588)4075421-2 s Labortechnik (DE-588)4123602-6 s b DE-604 Downing, A. Kristina edt Erscheint auch als Druck-Ausgabe 9781588292469 https://doi.org/10.1385/1592598099 Verlag URL des Erstveröffentlichers Volltext 1\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk |
spellingShingle | Protein NMR Techniques Life Sciences Biochemistry, general Life sciences Biochemistry Labortechnik (DE-588)4123602-6 gnd NMR-Spektroskopie (DE-588)4075421-2 gnd Proteine (DE-588)4076388-2 gnd |
subject_GND | (DE-588)4123602-6 (DE-588)4075421-2 (DE-588)4076388-2 (DE-588)4143413-4 |
title | Protein NMR Techniques |
title_auth | Protein NMR Techniques |
title_exact_search | Protein NMR Techniques |
title_full | Protein NMR Techniques edited by A. Kristina Downing |
title_fullStr | Protein NMR Techniques edited by A. Kristina Downing |
title_full_unstemmed | Protein NMR Techniques edited by A. Kristina Downing |
title_short | Protein NMR Techniques |
title_sort | protein nmr techniques |
topic | Life Sciences Biochemistry, general Life sciences Biochemistry Labortechnik (DE-588)4123602-6 gnd NMR-Spektroskopie (DE-588)4075421-2 gnd Proteine (DE-588)4076388-2 gnd |
topic_facet | Life Sciences Biochemistry, general Life sciences Biochemistry Labortechnik NMR-Spektroskopie Proteine Aufsatzsammlung |
url | https://doi.org/10.1385/1592598099 |
work_keys_str_mv | AT downingakristina proteinnmrtechniques |