Monoclonal Antibody Protocols:
Since the initial description of techniques to immortalize anti body-producing B-lymphocytes by fusion with tissue culture-adapted myeloma cells, methods have been developed to produce monoclonal antibodies of defined specificity in multiple animal species. Stable hybrids can be readily produced in...
Gespeichert in:
| Weitere Verfasser: | |
|---|---|
| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
Totowa, NJ
Humana Press
1995
|
| Schriftenreihe: | Methods in Molecular Biology™
45 |
| Schlagworte: | |
| Online-Zugang: | UBR01 Volltext |
| Zusammenfassung: | Since the initial description of techniques to immortalize anti body-producing B-lymphocytes by fusion with tissue culture-adapted myeloma cells, methods have been developed to produce monoclonal antibodies of defined specificity in multiple animal species. Stable hybrids can be readily produced in mice using a number of myeloma and hybridoma cell lines. To obviate the problem of identifying fusion partners in other animal species, xenohybrids have been produced using B-lymphocytes from the relevant species and mouse myeloma cells. The use of xenohybrids has minimized the problem of obtain ing stable antibody-producing hybrids in all species examined thus far. Although alternative techniques are being developed to produce monoclonal antibodies by molecular methods, hybridoma technol ogy will remain the technology of choice for producing monoclonal antibodies for a variety of applications in research and industry. The objective of Monoclonal Antibody Protocols is to provide investigators with a set of methods for producing and using mono clonal antibodies in biomedical, agricultural, and biological sciences. The book is not intended to provide methodology for all possible applications, but rather a series of methods presented in an easy-- follow format that can be used by new and established investiga tors, graduate and postgraduate fellows, and technical staff |
| Beschreibung: | 1 Online-Ressource (X, 264 p) |
| ISBN: | 9781592595327 |
| DOI: | 10.1385/0896033082 |
Internformat
MARC
| LEADER | 00000nmm a2200000zcb4500 | ||
|---|---|---|---|
| 001 | BV044951223 | ||
| 003 | DE-604 | ||
| 005 | 20180911 | ||
| 007 | cr|uuu---uuuuu | ||
| 008 | 180517s1995 |||| o||u| ||||||eng d | ||
| 020 | |a 9781592595327 |9 978-1-59259-532-7 | ||
| 024 | 7 | |a 10.1385/0896033082 |2 doi | |
| 035 | |a (ZDB-2-PRO)978-1-59259-532-7 | ||
| 035 | |a (OCoLC)1036379140 | ||
| 035 | |a (DE-599)BVBBV044951223 | ||
| 040 | |a DE-604 |b ger |e rda | ||
| 041 | 0 | |a eng | |
| 049 | |a DE-355 | ||
| 050 | 0 | |a QR186.85 | |
| 082 | 0 | |a 616.079 |2 23 | |
| 082 | 0 | |a 616.07/93 |2 20 | |
| 084 | |a WC 4150 |0 (DE-625)148092: |2 rvk | ||
| 084 | |a WF 9920 |0 (DE-625)148481: |2 rvk | ||
| 084 | |a CHE 880f |2 stub | ||
| 245 | 1 | 0 | |a Monoclonal Antibody Protocols |c edited by William C. Davis |
| 264 | 1 | |a Totowa, NJ |b Humana Press |c 1995 | |
| 300 | |a 1 Online-Ressource (X, 264 p) | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b c |2 rdamedia | ||
| 338 | |b cr |2 rdacarrier | ||
| 490 | 0 | |a Methods in Molecular Biology™ |v 45 | |
| 520 | |a Since the initial description of techniques to immortalize anti body-producing B-lymphocytes by fusion with tissue culture-adapted myeloma cells, methods have been developed to produce monoclonal antibodies of defined specificity in multiple animal species. Stable hybrids can be readily produced in mice using a number of myeloma and hybridoma cell lines. To obviate the problem of identifying fusion partners in other animal species, xenohybrids have been produced using B-lymphocytes from the relevant species and mouse myeloma cells. The use of xenohybrids has minimized the problem of obtain ing stable antibody-producing hybrids in all species examined thus far. Although alternative techniques are being developed to produce monoclonal antibodies by molecular methods, hybridoma technol ogy will remain the technology of choice for producing monoclonal antibodies for a variety of applications in research and industry. The objective of Monoclonal Antibody Protocols is to provide investigators with a set of methods for producing and using mono clonal antibodies in biomedical, agricultural, and biological sciences. The book is not intended to provide methodology for all possible applications, but rather a series of methods presented in an easy-- follow format that can be used by new and established investiga tors, graduate and postgraduate fellows, and technical staff | ||
| 650 | 4 | |a Biomedicine | |
| 650 | 4 | |a Immunology | |
| 650 | 4 | |a Medicine | |
| 650 | 4 | |a Immunology | |
| 650 | 0 | 7 | |a Methode |0 (DE-588)4038971-6 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Monoklonaler Antikörper |0 (DE-588)4040094-3 |2 gnd |9 rswk-swf |
| 655 | 7 | |8 1\p |0 (DE-588)4143413-4 |a Aufsatzsammlung |2 gnd-content | |
| 689 | 0 | 0 | |a Monoklonaler Antikörper |0 (DE-588)4040094-3 |D s |
| 689 | 0 | 1 | |a Methode |0 (DE-588)4038971-6 |D s |
| 689 | 0 | |C b |5 DE-604 | |
| 700 | 1 | |a Davis, William C. |4 edt | |
| 776 | 0 | 8 | |i Erscheint auch als |n Druck-Ausgabe |z 9780896033085 |
| 856 | 4 | 0 | |u https://doi.org/10.1385/0896033082 |x Verlag |z URL des Erstveröffentlichers |3 Volltext |
| 912 | |a ZDB-2-PRO | ||
| 999 | |a oai:aleph.bib-bvb.de:BVB01-030343981 | ||
| 883 | 1 | |8 1\p |a cgwrk |d 20201028 |q DE-101 |u https://d-nb.info/provenance/plan#cgwrk | |
| 966 | e | |u https://doi.org/10.1385/0896033082 |l UBR01 |p ZDB-2-PRO |x Verlag |3 Volltext | |
Datensatz im Suchindex
| _version_ | 1804178539576033280 |
|---|---|
| any_adam_object | |
| author2 | Davis, William C. |
| author2_role | edt |
| author2_variant | w c d wc wcd |
| author_facet | Davis, William C. |
| building | Verbundindex |
| bvnumber | BV044951223 |
| callnumber-first | Q - Science |
| callnumber-label | QR186 |
| callnumber-raw | QR186.85 |
| callnumber-search | QR186.85 |
| callnumber-sort | QR 3186.85 |
| callnumber-subject | QR - Microbiology |
| classification_rvk | WC 4150 WF 9920 |
| classification_tum | CHE 880f |
| collection | ZDB-2-PRO |
| ctrlnum | (ZDB-2-PRO)978-1-59259-532-7 (OCoLC)1036379140 (DE-599)BVBBV044951223 |
| dewey-full | 616.079 616.07/93 |
| dewey-hundreds | 600 - Technology (Applied sciences) |
| dewey-ones | 616 - Diseases |
| dewey-raw | 616.079 616.07/93 |
| dewey-search | 616.079 616.07/93 |
| dewey-sort | 3616.079 |
| dewey-tens | 610 - Medicine and health |
| discipline | Biologie Chemie Medizin |
| doi_str_mv | 10.1385/0896033082 |
| format | Electronic eBook |
| fullrecord | <?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>03286nmm a2200553zcb4500</leader><controlfield tag="001">BV044951223</controlfield><controlfield tag="003">DE-604</controlfield><controlfield tag="005">20180911 </controlfield><controlfield tag="007">cr|uuu---uuuuu</controlfield><controlfield tag="008">180517s1995 |||| o||u| ||||||eng d</controlfield><datafield tag="020" ind1=" " ind2=" "><subfield code="a">9781592595327</subfield><subfield code="9">978-1-59259-532-7</subfield></datafield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1385/0896033082</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(ZDB-2-PRO)978-1-59259-532-7</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(OCoLC)1036379140</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)BVBBV044951223</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-604</subfield><subfield code="b">ger</subfield><subfield code="e">rda</subfield></datafield><datafield tag="041" ind1="0" ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="049" ind1=" " ind2=" "><subfield code="a">DE-355</subfield></datafield><datafield tag="050" ind1=" " ind2="0"><subfield code="a">QR186.85</subfield></datafield><datafield tag="082" ind1="0" ind2=" "><subfield code="a">616.079</subfield><subfield code="2">23</subfield></datafield><datafield tag="082" ind1="0" ind2=" "><subfield code="a">616.07/93</subfield><subfield code="2">20</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">WC 4150</subfield><subfield code="0">(DE-625)148092:</subfield><subfield code="2">rvk</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">WF 9920</subfield><subfield code="0">(DE-625)148481:</subfield><subfield code="2">rvk</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">CHE 880f</subfield><subfield code="2">stub</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Monoclonal Antibody Protocols</subfield><subfield code="c">edited by William C. Davis</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="a">Totowa, NJ</subfield><subfield code="b">Humana Press</subfield><subfield code="c">1995</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">1 Online-Ressource (X, 264 p)</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="490" ind1="0" ind2=" "><subfield code="a">Methods in Molecular Biology™</subfield><subfield code="v">45</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Since the initial description of techniques to immortalize anti body-producing B-lymphocytes by fusion with tissue culture-adapted myeloma cells, methods have been developed to produce monoclonal antibodies of defined specificity in multiple animal species. Stable hybrids can be readily produced in mice using a number of myeloma and hybridoma cell lines. To obviate the problem of identifying fusion partners in other animal species, xenohybrids have been produced using B-lymphocytes from the relevant species and mouse myeloma cells. The use of xenohybrids has minimized the problem of obtain ing stable antibody-producing hybrids in all species examined thus far. Although alternative techniques are being developed to produce monoclonal antibodies by molecular methods, hybridoma technol ogy will remain the technology of choice for producing monoclonal antibodies for a variety of applications in research and industry. The objective of Monoclonal Antibody Protocols is to provide investigators with a set of methods for producing and using mono clonal antibodies in biomedical, agricultural, and biological sciences. The book is not intended to provide methodology for all possible applications, but rather a series of methods presented in an easy-- follow format that can be used by new and established investiga tors, graduate and postgraduate fellows, and technical staff</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Biomedicine</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Immunology</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Medicine</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Immunology</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Methode</subfield><subfield code="0">(DE-588)4038971-6</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Monoklonaler Antikörper</subfield><subfield code="0">(DE-588)4040094-3</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="655" ind1=" " ind2="7"><subfield code="8">1\p</subfield><subfield code="0">(DE-588)4143413-4</subfield><subfield code="a">Aufsatzsammlung</subfield><subfield code="2">gnd-content</subfield></datafield><datafield tag="689" ind1="0" ind2="0"><subfield code="a">Monoklonaler Antikörper</subfield><subfield code="0">(DE-588)4040094-3</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2="1"><subfield code="a">Methode</subfield><subfield code="0">(DE-588)4038971-6</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2=" "><subfield code="C">b</subfield><subfield code="5">DE-604</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Davis, William C.</subfield><subfield code="4">edt</subfield></datafield><datafield tag="776" ind1="0" ind2="8"><subfield code="i">Erscheint auch als</subfield><subfield code="n">Druck-Ausgabe</subfield><subfield code="z">9780896033085</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://doi.org/10.1385/0896033082</subfield><subfield code="x">Verlag</subfield><subfield code="z">URL des Erstveröffentlichers</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">ZDB-2-PRO</subfield></datafield><datafield tag="999" ind1=" " ind2=" "><subfield code="a">oai:aleph.bib-bvb.de:BVB01-030343981</subfield></datafield><datafield tag="883" ind1="1" ind2=" "><subfield code="8">1\p</subfield><subfield code="a">cgwrk</subfield><subfield code="d">20201028</subfield><subfield code="q">DE-101</subfield><subfield code="u">https://d-nb.info/provenance/plan#cgwrk</subfield></datafield><datafield tag="966" ind1="e" ind2=" "><subfield code="u">https://doi.org/10.1385/0896033082</subfield><subfield code="l">UBR01</subfield><subfield code="p">ZDB-2-PRO</subfield><subfield code="x">Verlag</subfield><subfield code="3">Volltext</subfield></datafield></record></collection> |
| genre | 1\p (DE-588)4143413-4 Aufsatzsammlung gnd-content |
| genre_facet | Aufsatzsammlung |
| id | DE-604.BV044951223 |
| illustrated | Not Illustrated |
| indexdate | 2024-07-10T08:05:35Z |
| institution | BVB |
| isbn | 9781592595327 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-030343981 |
| oclc_num | 1036379140 |
| open_access_boolean | |
| owner | DE-355 DE-BY-UBR |
| owner_facet | DE-355 DE-BY-UBR |
| physical | 1 Online-Ressource (X, 264 p) |
| psigel | ZDB-2-PRO |
| publishDate | 1995 |
| publishDateSearch | 1995 |
| publishDateSort | 1995 |
| publisher | Humana Press |
| record_format | marc |
| series2 | Methods in Molecular Biology™ |
| spelling | Monoclonal Antibody Protocols edited by William C. Davis Totowa, NJ Humana Press 1995 1 Online-Ressource (X, 264 p) txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology™ 45 Since the initial description of techniques to immortalize anti body-producing B-lymphocytes by fusion with tissue culture-adapted myeloma cells, methods have been developed to produce monoclonal antibodies of defined specificity in multiple animal species. Stable hybrids can be readily produced in mice using a number of myeloma and hybridoma cell lines. To obviate the problem of identifying fusion partners in other animal species, xenohybrids have been produced using B-lymphocytes from the relevant species and mouse myeloma cells. The use of xenohybrids has minimized the problem of obtain ing stable antibody-producing hybrids in all species examined thus far. Although alternative techniques are being developed to produce monoclonal antibodies by molecular methods, hybridoma technol ogy will remain the technology of choice for producing monoclonal antibodies for a variety of applications in research and industry. The objective of Monoclonal Antibody Protocols is to provide investigators with a set of methods for producing and using mono clonal antibodies in biomedical, agricultural, and biological sciences. The book is not intended to provide methodology for all possible applications, but rather a series of methods presented in an easy-- follow format that can be used by new and established investiga tors, graduate and postgraduate fellows, and technical staff Biomedicine Immunology Medicine Methode (DE-588)4038971-6 gnd rswk-swf Monoklonaler Antikörper (DE-588)4040094-3 gnd rswk-swf 1\p (DE-588)4143413-4 Aufsatzsammlung gnd-content Monoklonaler Antikörper (DE-588)4040094-3 s Methode (DE-588)4038971-6 s b DE-604 Davis, William C. edt Erscheint auch als Druck-Ausgabe 9780896033085 https://doi.org/10.1385/0896033082 Verlag URL des Erstveröffentlichers Volltext 1\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk |
| spellingShingle | Monoclonal Antibody Protocols Biomedicine Immunology Medicine Methode (DE-588)4038971-6 gnd Monoklonaler Antikörper (DE-588)4040094-3 gnd |
| subject_GND | (DE-588)4038971-6 (DE-588)4040094-3 (DE-588)4143413-4 |
| title | Monoclonal Antibody Protocols |
| title_auth | Monoclonal Antibody Protocols |
| title_exact_search | Monoclonal Antibody Protocols |
| title_full | Monoclonal Antibody Protocols edited by William C. Davis |
| title_fullStr | Monoclonal Antibody Protocols edited by William C. Davis |
| title_full_unstemmed | Monoclonal Antibody Protocols edited by William C. Davis |
| title_short | Monoclonal Antibody Protocols |
| title_sort | monoclonal antibody protocols |
| topic | Biomedicine Immunology Medicine Methode (DE-588)4038971-6 gnd Monoklonaler Antikörper (DE-588)4040094-3 gnd |
| topic_facet | Biomedicine Immunology Medicine Methode Monoklonaler Antikörper Aufsatzsammlung |
| url | https://doi.org/10.1385/0896033082 |
| work_keys_str_mv | AT daviswilliamc monoclonalantibodyprotocols |