Plant Argonaute Proteins: Methods and Protocols
This volume provides the reader with step–by–step methods to study plant ARGONAUTE (AGO) functions. Chapters in Plant Argonaute Poteins: Methods and Protocols summarize the main biochemical, molecular and cytological methods to study AGO–small RNA complexes and their interactions with target RNAs, A...
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Weitere Verfasser: | |
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Format: | Elektronisch E-Book |
Sprache: | English |
Veröffentlicht: |
New York, NY
Springer New York
2017
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Schriftenreihe: | Methods in Molecular Biology
1640 |
Schlagworte: | |
Online-Zugang: | UBR01 TUM01 Volltext |
Zusammenfassung: | This volume provides the reader with step–by–step methods to study plant ARGONAUTE (AGO) functions. Chapters in Plant Argonaute Poteins: Methods and Protocols summarize the main biochemical, molecular and cytological methods to study AGO–small RNA complexes and their interactions with target RNAs, AGO subcellular localization, AGO association with polysomes, AGO role in meiosis and DNA repair, methods for the identification, cloning and characterization of AGO genes in different plant species, and non–radioactive protocols for small RNA detection. Finally, a series of chapters describing computational methods to study plant AGO function and evolution are provided. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Plant Argonaute Proteins: Methods and Protocols aims to deliver the most complete and updated list of protocols to study plant AGO function |
Beschreibung: | 1 Online-Ressource (XI, 297 p. 47 illus., 27 illus. in color) |
ISBN: | 9781493971657 |
DOI: | 10.1007/978-1-4939-7165-7 |
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dewey-hundreds | 500 - Natural sciences and mathematics |
dewey-ones | 580 - Plants |
dewey-raw | 580 |
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dewey-sort | 3580 |
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spelling | Plant Argonaute Proteins Methods and Protocols edited by Alberto Carbonell New York, NY Springer New York 2017 1 Online-Ressource (XI, 297 p. 47 illus., 27 illus. in color) txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology 1640 This volume provides the reader with step–by–step methods to study plant ARGONAUTE (AGO) functions. Chapters in Plant Argonaute Poteins: Methods and Protocols summarize the main biochemical, molecular and cytological methods to study AGO–small RNA complexes and their interactions with target RNAs, AGO subcellular localization, AGO association with polysomes, AGO role in meiosis and DNA repair, methods for the identification, cloning and characterization of AGO genes in different plant species, and non–radioactive protocols for small RNA detection. Finally, a series of chapters describing computational methods to study plant AGO function and evolution are provided. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Plant Argonaute Proteins: Methods and Protocols aims to deliver the most complete and updated list of protocols to study plant AGO function Life Sciences Plant Sciences Life sciences Plant science Botany Carbonell, Alberto edt Erscheint auch als Druck-Ausgabe 9781493971640 https://doi.org/10.1007/978-1-4939-7165-7 Verlag URL des Erstveröffentlichers Volltext |
spellingShingle | Plant Argonaute Proteins Methods and Protocols Life Sciences Plant Sciences Life sciences Plant science Botany |
title | Plant Argonaute Proteins Methods and Protocols |
title_auth | Plant Argonaute Proteins Methods and Protocols |
title_exact_search | Plant Argonaute Proteins Methods and Protocols |
title_full | Plant Argonaute Proteins Methods and Protocols edited by Alberto Carbonell |
title_fullStr | Plant Argonaute Proteins Methods and Protocols edited by Alberto Carbonell |
title_full_unstemmed | Plant Argonaute Proteins Methods and Protocols edited by Alberto Carbonell |
title_short | Plant Argonaute Proteins |
title_sort | plant argonaute proteins methods and protocols |
title_sub | Methods and Protocols |
topic | Life Sciences Plant Sciences Life sciences Plant science Botany |
topic_facet | Life Sciences Plant Sciences Life sciences Plant science Botany |
url | https://doi.org/10.1007/978-1-4939-7165-7 |
work_keys_str_mv | AT carbonellalberto plantargonauteproteinsmethodsandprotocols |