Insoluble Proteins: Methods and Protocols
With insolubility proving to be one of the most crippling bottlenecks in the protein production and purification process, this volume serves to aid researchers working in the recombinant protein production field by describing a wide number of protocols and examples. Insoluble Proteins: Methods and P...
Gespeichert in:
| Weitere Verfasser: | |
|---|---|
| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
New York, NY
Springer New York
2015
|
| Schriftenreihe: | Methods in Molecular Biology, Methods and Protocols
1258 |
| Schlagworte: | |
| Online-Zugang: | UBR01 TUM01 Volltext |
| Zusammenfassung: | With insolubility proving to be one of the most crippling bottlenecks in the protein production and purification process, this volume serves to aid researchers working in the recombinant protein production field by describing a wide number of protocols and examples. Insoluble Proteins: Methods and Protocols includes chapters that describe not only the recombinant protein production in different expression systems but also different purification and characterization methods to finally obtain these difficult-to-obtain proteins. Beginning with protein production methods using both prokaryotic and eukaryotic expression systems, the book continues with purification protocols using insoluble proteins, the characterization of insoluble proteins, as well as a general overview of interesting applications of insoluble proteins. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and practical, Insoluble Proteins: Methods and Protocols aims to provide the scientific community with detailed and reliable state-of-the-art protocols that are used in order to successfully produce and purify recombinant proteins prone to aggregate |
| Beschreibung: | 1 Online-Ressource (XVI, 425 p. 72 illus., 41 illus. in color) |
| ISBN: | 9781493922055 |
| DOI: | 10.1007/978-1-4939-2205-5 |
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| dewey-full | 572.6 |
| dewey-hundreds | 500 - Natural sciences and mathematics |
| dewey-ones | 572 - Biochemistry |
| dewey-raw | 572.6 |
| dewey-search | 572.6 |
| dewey-sort | 3572.6 |
| dewey-tens | 570 - Biology |
| discipline | Biologie |
| doi_str_mv | 10.1007/978-1-4939-2205-5 |
| format | Electronic eBook |
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| id | DE-604.BV044951219 |
| illustrated | Not Illustrated |
| indexdate | 2024-07-10T08:05:35Z |
| institution | BVB |
| isbn | 9781493922055 |
| language | English |
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| physical | 1 Online-Ressource (XVI, 425 p. 72 illus., 41 illus. in color) |
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| spelling | Insoluble Proteins Methods and Protocols edited by Elena García-Fruitós New York, NY Springer New York 2015 1 Online-Ressource (XVI, 425 p. 72 illus., 41 illus. in color) txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology, Methods and Protocols 1258 With insolubility proving to be one of the most crippling bottlenecks in the protein production and purification process, this volume serves to aid researchers working in the recombinant protein production field by describing a wide number of protocols and examples. Insoluble Proteins: Methods and Protocols includes chapters that describe not only the recombinant protein production in different expression systems but also different purification and characterization methods to finally obtain these difficult-to-obtain proteins. Beginning with protein production methods using both prokaryotic and eukaryotic expression systems, the book continues with purification protocols using insoluble proteins, the characterization of insoluble proteins, as well as a general overview of interesting applications of insoluble proteins. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and practical, Insoluble Proteins: Methods and Protocols aims to provide the scientific community with detailed and reliable state-of-the-art protocols that are used in order to successfully produce and purify recombinant proteins prone to aggregate Life Sciences Protein Science Life sciences Proteins García-Fruitós, Elena edt Erscheint auch als Druck-Ausgabe 9781493922048 https://doi.org/10.1007/978-1-4939-2205-5 Verlag URL des Erstveröffentlichers Volltext |
| spellingShingle | Insoluble Proteins Methods and Protocols Life Sciences Protein Science Life sciences Proteins |
| title | Insoluble Proteins Methods and Protocols |
| title_auth | Insoluble Proteins Methods and Protocols |
| title_exact_search | Insoluble Proteins Methods and Protocols |
| title_full | Insoluble Proteins Methods and Protocols edited by Elena García-Fruitós |
| title_fullStr | Insoluble Proteins Methods and Protocols edited by Elena García-Fruitós |
| title_full_unstemmed | Insoluble Proteins Methods and Protocols edited by Elena García-Fruitós |
| title_short | Insoluble Proteins |
| title_sort | insoluble proteins methods and protocols |
| title_sub | Methods and Protocols |
| topic | Life Sciences Protein Science Life sciences Proteins |
| topic_facet | Life Sciences Protein Science Life sciences Proteins |
| url | https://doi.org/10.1007/978-1-4939-2205-5 |
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