DNA Repair Protocols: Mammalian Systems
In this second edition of a much praised laboratory manual devoted to eukaryotic systems, Daryl S. Henderson has refocused the book on mammalian cells, adding fourteen entirely new chapters and extensively revising many of the remaining chapters. The authors address a broad range of questions about...
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| Weitere Verfasser: | |
|---|---|
| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
Totowa, NJ
Humana Press
2006
|
| Ausgabe: | Second Edition |
| Schriftenreihe: | Methods in Molecular Biology™
314 |
| Schlagworte: | |
| Online-Zugang: | UBR01 TUM01 Volltext |
| Zusammenfassung: | In this second edition of a much praised laboratory manual devoted to eukaryotic systems, Daryl S. Henderson has refocused the book on mammalian cells, adding fourteen entirely new chapters and extensively revising many of the remaining chapters. The authors address a broad range of questions about practical mammalian DNA repair, including such arcana as "what is radioresistant DNA synthesis and how is it measured?" The techniques presented are readily reproducible and offer cutting-edge methods for cytogenetic analysis, measuring the cellular response to ionizing radiation, detecting single-strand (nicks) and double-strand DNA breaks, detecting the presence of "adducted" bases in DNA, and preparing mismatch repair (MMR) plasmid substrates. Among the highlights are excellent coverage of both base excision repair (BER) and nucleotide excision repair (NER), useful assays for identifying and quantifying UV-induced DNA lesions and DNA breakage, gene therapy, environmental mutagenesis and cancer, and gene targeting. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Authoritative and highly practical, DNA Repair Protocols: Mammalian Systems, Second Edition, offers investigators a wide variety of productive methods to explore and make new discoveries in the world of mammalian DNA repair |
| Beschreibung: | 1 Online-Ressource (XVIII, 498 p) |
| ISBN: | 9781592599738 |
| DOI: | 10.1385/1592599737 |
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| spelling | DNA Repair Protocols Mammalian Systems edited by Daryl S. Henderson Second Edition Totowa, NJ Humana Press 2006 1 Online-Ressource (XVIII, 498 p) txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology™ 314 In this second edition of a much praised laboratory manual devoted to eukaryotic systems, Daryl S. Henderson has refocused the book on mammalian cells, adding fourteen entirely new chapters and extensively revising many of the remaining chapters. The authors address a broad range of questions about practical mammalian DNA repair, including such arcana as "what is radioresistant DNA synthesis and how is it measured?" The techniques presented are readily reproducible and offer cutting-edge methods for cytogenetic analysis, measuring the cellular response to ionizing radiation, detecting single-strand (nicks) and double-strand DNA breaks, detecting the presence of "adducted" bases in DNA, and preparing mismatch repair (MMR) plasmid substrates. Among the highlights are excellent coverage of both base excision repair (BER) and nucleotide excision repair (NER), useful assays for identifying and quantifying UV-induced DNA lesions and DNA breakage, gene therapy, environmental mutagenesis and cancer, and gene targeting. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Authoritative and highly practical, DNA Repair Protocols: Mammalian Systems, Second Edition, offers investigators a wide variety of productive methods to explore and make new discoveries in the world of mammalian DNA repair Life Sciences Biochemistry, general Cell Biology Life sciences Biochemistry Cell biology Methode (DE-588)4038971-6 gnd rswk-swf Chemisches Verfahren (DE-588)4454573-3 gnd rswk-swf DNS-Reparatur (DE-588)4150347-8 gnd rswk-swf Säugetiere (DE-588)4051253-8 gnd rswk-swf Molekularbiologie (DE-588)4039983-7 gnd rswk-swf 1\p (DE-588)4143413-4 Aufsatzsammlung gnd-content DNS-Reparatur (DE-588)4150347-8 s Chemisches Verfahren (DE-588)4454573-3 s DE-604 Säugetiere (DE-588)4051253-8 s Molekularbiologie (DE-588)4039983-7 s Methode (DE-588)4038971-6 s b DE-604 Henderson, Daryl S. edt Erscheint auch als Druck-Ausgabe 9781588295132 https://doi.org/10.1385/1592599737 Verlag URL des Erstveröffentlichers Volltext 1\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk |
| spellingShingle | DNA Repair Protocols Mammalian Systems Life Sciences Biochemistry, general Cell Biology Life sciences Biochemistry Cell biology Methode (DE-588)4038971-6 gnd Chemisches Verfahren (DE-588)4454573-3 gnd DNS-Reparatur (DE-588)4150347-8 gnd Säugetiere (DE-588)4051253-8 gnd Molekularbiologie (DE-588)4039983-7 gnd |
| subject_GND | (DE-588)4038971-6 (DE-588)4454573-3 (DE-588)4150347-8 (DE-588)4051253-8 (DE-588)4039983-7 (DE-588)4143413-4 |
| title | DNA Repair Protocols Mammalian Systems |
| title_auth | DNA Repair Protocols Mammalian Systems |
| title_exact_search | DNA Repair Protocols Mammalian Systems |
| title_full | DNA Repair Protocols Mammalian Systems edited by Daryl S. Henderson |
| title_fullStr | DNA Repair Protocols Mammalian Systems edited by Daryl S. Henderson |
| title_full_unstemmed | DNA Repair Protocols Mammalian Systems edited by Daryl S. Henderson |
| title_short | DNA Repair Protocols |
| title_sort | dna repair protocols mammalian systems |
| title_sub | Mammalian Systems |
| topic | Life Sciences Biochemistry, general Cell Biology Life sciences Biochemistry Cell biology Methode (DE-588)4038971-6 gnd Chemisches Verfahren (DE-588)4454573-3 gnd DNS-Reparatur (DE-588)4150347-8 gnd Säugetiere (DE-588)4051253-8 gnd Molekularbiologie (DE-588)4039983-7 gnd |
| topic_facet | Life Sciences Biochemistry, general Cell Biology Life sciences Biochemistry Cell biology Methode Chemisches Verfahren DNS-Reparatur Säugetiere Molekularbiologie Aufsatzsammlung |
| url | https://doi.org/10.1385/1592599737 |
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