Immunoelectron Microscopy: Methods and Protocols
Immunoelectron microscopy is a key technique that bridges the information gap between biochemistry, molecular biology, and ultrastructural studies placing macromolecular functions within a cellular context. In Immunoelectron Microscopy: Methods and Protocols, expert researchers combine the tools of...
Gespeichert in:
| Weitere Verfasser: | , |
|---|---|
| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
Totowa, NJ
Humana Press
2010
|
| Schriftenreihe: | Methods in Molecular Biology, Methods and Protocols
657 |
| Schlagworte: | |
| Online-Zugang: | UBR01 TUM01 Volltext |
| Zusammenfassung: | Immunoelectron microscopy is a key technique that bridges the information gap between biochemistry, molecular biology, and ultrastructural studies placing macromolecular functions within a cellular context. In Immunoelectron Microscopy: Methods and Protocols, expert researchers combine the tools of the molecular biologist with those of the microscopist. From the molecular biology toolbox, this volume presents methods for antigen production by protein expression in bacterial cells, methods for epitope tagged protein expression in plant and animal cells allowing protein localization in the absence of protein specific antibodies as well as methods for the production of anti-peptide, monoclonal, and polyclonal antibodies. From the microscopy toolbox, sample preparation methods for cells, plant, and animal tissue are presented. Both cryo-methods, which have the advantage of retaining protein antigenicity at the expense of ultrastructural integrity, as well as chemical fixation methods that maintain structural integrity while sacrificing protein antigenicity have been included, with chapters examining various aspects of immunogold labeling. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and essential, Immunoelectron Microscopy: Methods and Protocols seeks to facilitate an increased understanding of structure function relationships |
| Beschreibung: | 1 Online-Ressource (XI, 352 p) |
| ISBN: | 9781607617839 |
| DOI: | 10.1007/978-1-60761-783-9 |
Internformat
MARC
| LEADER | 00000nmm a2200000zcb4500 | ||
|---|---|---|---|
| 001 | BV044951171 | ||
| 003 | DE-604 | ||
| 005 | 00000000000000.0 | ||
| 007 | cr|uuu---uuuuu | ||
| 008 | 180517s2010 |||| o||u| ||||||eng d | ||
| 020 | |a 9781607617839 |9 978-1-60761-783-9 | ||
| 024 | 7 | |a 10.1007/978-1-60761-783-9 |2 doi | |
| 035 | |a (ZDB-2-PRO)978-1-60761-783-9 | ||
| 035 | |a (OCoLC)1184344179 | ||
| 035 | |a (DE-599)BVBBV044951171 | ||
| 040 | |a DE-604 |b ger |e rda | ||
| 041 | 0 | |a eng | |
| 049 | |a DE-355 |a DE-91 | ||
| 082 | 0 | |a 571.6 |2 23 | |
| 084 | |a WC 3100 |0 (DE-625)148081: |2 rvk | ||
| 245 | 1 | 0 | |a Immunoelectron Microscopy |b Methods and Protocols |c edited by Steven D. Schwartzbach, Tetsuaki Osafune |
| 264 | 1 | |a Totowa, NJ |b Humana Press |c 2010 | |
| 300 | |a 1 Online-Ressource (XI, 352 p) | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b c |2 rdamedia | ||
| 338 | |b cr |2 rdacarrier | ||
| 490 | 0 | |a Methods in Molecular Biology, Methods and Protocols |v 657 | |
| 520 | |a Immunoelectron microscopy is a key technique that bridges the information gap between biochemistry, molecular biology, and ultrastructural studies placing macromolecular functions within a cellular context. In Immunoelectron Microscopy: Methods and Protocols, expert researchers combine the tools of the molecular biologist with those of the microscopist. From the molecular biology toolbox, this volume presents methods for antigen production by protein expression in bacterial cells, methods for epitope tagged protein expression in plant and animal cells allowing protein localization in the absence of protein specific antibodies as well as methods for the production of anti-peptide, monoclonal, and polyclonal antibodies. From the microscopy toolbox, sample preparation methods for cells, plant, and animal tissue are presented. Both cryo-methods, which have the advantage of retaining protein antigenicity at the expense of ultrastructural integrity, as well as chemical fixation methods that maintain structural integrity while sacrificing protein antigenicity have been included, with chapters examining various aspects of immunogold labeling. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and essential, Immunoelectron Microscopy: Methods and Protocols seeks to facilitate an increased understanding of structure function relationships | ||
| 650 | 4 | |a Life Sciences | |
| 650 | 4 | |a Cell Biology | |
| 650 | 4 | |a Immunology | |
| 650 | 4 | |a Antibodies | |
| 650 | 4 | |a Life sciences | |
| 650 | 4 | |a Immunology | |
| 650 | 4 | |a Antibodies | |
| 650 | 4 | |a Cell biology | |
| 650 | 0 | 7 | |a Immunelektronenmikroskopie |0 (DE-588)4161370-3 |2 gnd |9 rswk-swf |
| 689 | 0 | 0 | |a Immunelektronenmikroskopie |0 (DE-588)4161370-3 |D s |
| 689 | 0 | |8 1\p |5 DE-604 | |
| 700 | 1 | |a Schwartzbach, Steven D. |4 edt | |
| 700 | 1 | |a Osafune, Tetsuaki |4 edt | |
| 776 | 0 | 8 | |i Erscheint auch als |n Druck-Ausgabe |z 9781607617822 |
| 856 | 4 | 0 | |u https://doi.org/10.1007/978-1-60761-783-9 |x Verlag |z URL des Erstveröffentlichers |3 Volltext |
| 912 | |a ZDB-2-PRO | ||
| 999 | |a oai:aleph.bib-bvb.de:BVB01-030343929 | ||
| 883 | 1 | |8 1\p |a cgwrk |d 20201028 |q DE-101 |u https://d-nb.info/provenance/plan#cgwrk | |
| 966 | e | |u https://doi.org/10.1007/978-1-60761-783-9 |l UBR01 |p ZDB-2-PRO |x Verlag |3 Volltext | |
| 966 | e | |u https://doi.org/10.1007/978-1-60761-783-9 |l TUM01 |p ZDB-2-PRO |x Verlag |3 Volltext | |
Datensatz im Suchindex
| _version_ | 1804178539476418560 |
|---|---|
| any_adam_object | |
| author2 | Schwartzbach, Steven D. Osafune, Tetsuaki |
| author2_role | edt edt |
| author2_variant | s d s sd sds t o to |
| author_facet | Schwartzbach, Steven D. Osafune, Tetsuaki |
| building | Verbundindex |
| bvnumber | BV044951171 |
| classification_rvk | WC 3100 |
| collection | ZDB-2-PRO |
| ctrlnum | (ZDB-2-PRO)978-1-60761-783-9 (OCoLC)1184344179 (DE-599)BVBBV044951171 |
| dewey-full | 571.6 |
| dewey-hundreds | 500 - Natural sciences and mathematics |
| dewey-ones | 571 - Physiology & related subjects |
| dewey-raw | 571.6 |
| dewey-search | 571.6 |
| dewey-sort | 3571.6 |
| dewey-tens | 570 - Biology |
| discipline | Biologie |
| doi_str_mv | 10.1007/978-1-60761-783-9 |
| format | Electronic eBook |
| fullrecord | <?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>03558nmm a2200541zcb4500</leader><controlfield tag="001">BV044951171</controlfield><controlfield tag="003">DE-604</controlfield><controlfield tag="005">00000000000000.0</controlfield><controlfield tag="007">cr|uuu---uuuuu</controlfield><controlfield tag="008">180517s2010 |||| o||u| ||||||eng d</controlfield><datafield tag="020" ind1=" " ind2=" "><subfield code="a">9781607617839</subfield><subfield code="9">978-1-60761-783-9</subfield></datafield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1007/978-1-60761-783-9</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(ZDB-2-PRO)978-1-60761-783-9</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(OCoLC)1184344179</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)BVBBV044951171</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-604</subfield><subfield code="b">ger</subfield><subfield code="e">rda</subfield></datafield><datafield tag="041" ind1="0" ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="049" ind1=" " ind2=" "><subfield code="a">DE-355</subfield><subfield code="a">DE-91</subfield></datafield><datafield tag="082" ind1="0" ind2=" "><subfield code="a">571.6</subfield><subfield code="2">23</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">WC 3100</subfield><subfield code="0">(DE-625)148081:</subfield><subfield code="2">rvk</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Immunoelectron Microscopy</subfield><subfield code="b">Methods and Protocols</subfield><subfield code="c">edited by Steven D. Schwartzbach, Tetsuaki Osafune</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="a">Totowa, NJ</subfield><subfield code="b">Humana Press</subfield><subfield code="c">2010</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">1 Online-Ressource (XI, 352 p)</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="490" ind1="0" ind2=" "><subfield code="a">Methods in Molecular Biology, Methods and Protocols</subfield><subfield code="v">657</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Immunoelectron microscopy is a key technique that bridges the information gap between biochemistry, molecular biology, and ultrastructural studies placing macromolecular functions within a cellular context. In Immunoelectron Microscopy: Methods and Protocols, expert researchers combine the tools of the molecular biologist with those of the microscopist. From the molecular biology toolbox, this volume presents methods for antigen production by protein expression in bacterial cells, methods for epitope tagged protein expression in plant and animal cells allowing protein localization in the absence of protein specific antibodies as well as methods for the production of anti-peptide, monoclonal, and polyclonal antibodies. From the microscopy toolbox, sample preparation methods for cells, plant, and animal tissue are presented. Both cryo-methods, which have the advantage of retaining protein antigenicity at the expense of ultrastructural integrity, as well as chemical fixation methods that maintain structural integrity while sacrificing protein antigenicity have been included, with chapters examining various aspects of immunogold labeling. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and essential, Immunoelectron Microscopy: Methods and Protocols seeks to facilitate an increased understanding of structure function relationships</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Life Sciences</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Cell Biology</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Immunology</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Antibodies</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Life sciences</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Immunology</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Antibodies</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Cell biology</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Immunelektronenmikroskopie</subfield><subfield code="0">(DE-588)4161370-3</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="689" ind1="0" ind2="0"><subfield code="a">Immunelektronenmikroskopie</subfield><subfield code="0">(DE-588)4161370-3</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2=" "><subfield code="8">1\p</subfield><subfield code="5">DE-604</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Schwartzbach, Steven D.</subfield><subfield code="4">edt</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Osafune, Tetsuaki</subfield><subfield code="4">edt</subfield></datafield><datafield tag="776" ind1="0" ind2="8"><subfield code="i">Erscheint auch als</subfield><subfield code="n">Druck-Ausgabe</subfield><subfield code="z">9781607617822</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://doi.org/10.1007/978-1-60761-783-9</subfield><subfield code="x">Verlag</subfield><subfield code="z">URL des Erstveröffentlichers</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">ZDB-2-PRO</subfield></datafield><datafield tag="999" ind1=" " ind2=" "><subfield code="a">oai:aleph.bib-bvb.de:BVB01-030343929</subfield></datafield><datafield tag="883" ind1="1" ind2=" "><subfield code="8">1\p</subfield><subfield code="a">cgwrk</subfield><subfield code="d">20201028</subfield><subfield code="q">DE-101</subfield><subfield code="u">https://d-nb.info/provenance/plan#cgwrk</subfield></datafield><datafield tag="966" ind1="e" ind2=" "><subfield code="u">https://doi.org/10.1007/978-1-60761-783-9</subfield><subfield code="l">UBR01</subfield><subfield code="p">ZDB-2-PRO</subfield><subfield code="x">Verlag</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="966" ind1="e" ind2=" "><subfield code="u">https://doi.org/10.1007/978-1-60761-783-9</subfield><subfield code="l">TUM01</subfield><subfield code="p">ZDB-2-PRO</subfield><subfield code="x">Verlag</subfield><subfield code="3">Volltext</subfield></datafield></record></collection> |
| id | DE-604.BV044951171 |
| illustrated | Not Illustrated |
| indexdate | 2024-07-10T08:05:35Z |
| institution | BVB |
| isbn | 9781607617839 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-030343929 |
| oclc_num | 1184344179 |
| open_access_boolean | |
| owner | DE-355 DE-BY-UBR DE-91 DE-BY-TUM |
| owner_facet | DE-355 DE-BY-UBR DE-91 DE-BY-TUM |
| physical | 1 Online-Ressource (XI, 352 p) |
| psigel | ZDB-2-PRO |
| publishDate | 2010 |
| publishDateSearch | 2010 |
| publishDateSort | 2010 |
| publisher | Humana Press |
| record_format | marc |
| series2 | Methods in Molecular Biology, Methods and Protocols |
| spelling | Immunoelectron Microscopy Methods and Protocols edited by Steven D. Schwartzbach, Tetsuaki Osafune Totowa, NJ Humana Press 2010 1 Online-Ressource (XI, 352 p) txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology, Methods and Protocols 657 Immunoelectron microscopy is a key technique that bridges the information gap between biochemistry, molecular biology, and ultrastructural studies placing macromolecular functions within a cellular context. In Immunoelectron Microscopy: Methods and Protocols, expert researchers combine the tools of the molecular biologist with those of the microscopist. From the molecular biology toolbox, this volume presents methods for antigen production by protein expression in bacterial cells, methods for epitope tagged protein expression in plant and animal cells allowing protein localization in the absence of protein specific antibodies as well as methods for the production of anti-peptide, monoclonal, and polyclonal antibodies. From the microscopy toolbox, sample preparation methods for cells, plant, and animal tissue are presented. Both cryo-methods, which have the advantage of retaining protein antigenicity at the expense of ultrastructural integrity, as well as chemical fixation methods that maintain structural integrity while sacrificing protein antigenicity have been included, with chapters examining various aspects of immunogold labeling. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and essential, Immunoelectron Microscopy: Methods and Protocols seeks to facilitate an increased understanding of structure function relationships Life Sciences Cell Biology Immunology Antibodies Life sciences Cell biology Immunelektronenmikroskopie (DE-588)4161370-3 gnd rswk-swf Immunelektronenmikroskopie (DE-588)4161370-3 s 1\p DE-604 Schwartzbach, Steven D. edt Osafune, Tetsuaki edt Erscheint auch als Druck-Ausgabe 9781607617822 https://doi.org/10.1007/978-1-60761-783-9 Verlag URL des Erstveröffentlichers Volltext 1\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk |
| spellingShingle | Immunoelectron Microscopy Methods and Protocols Life Sciences Cell Biology Immunology Antibodies Life sciences Cell biology Immunelektronenmikroskopie (DE-588)4161370-3 gnd |
| subject_GND | (DE-588)4161370-3 |
| title | Immunoelectron Microscopy Methods and Protocols |
| title_auth | Immunoelectron Microscopy Methods and Protocols |
| title_exact_search | Immunoelectron Microscopy Methods and Protocols |
| title_full | Immunoelectron Microscopy Methods and Protocols edited by Steven D. Schwartzbach, Tetsuaki Osafune |
| title_fullStr | Immunoelectron Microscopy Methods and Protocols edited by Steven D. Schwartzbach, Tetsuaki Osafune |
| title_full_unstemmed | Immunoelectron Microscopy Methods and Protocols edited by Steven D. Schwartzbach, Tetsuaki Osafune |
| title_short | Immunoelectron Microscopy |
| title_sort | immunoelectron microscopy methods and protocols |
| title_sub | Methods and Protocols |
| topic | Life Sciences Cell Biology Immunology Antibodies Life sciences Cell biology Immunelektronenmikroskopie (DE-588)4161370-3 gnd |
| topic_facet | Life Sciences Cell Biology Immunology Antibodies Life sciences Cell biology Immunelektronenmikroskopie |
| url | https://doi.org/10.1007/978-1-60761-783-9 |
| work_keys_str_mv | AT schwartzbachstevend immunoelectronmicroscopymethodsandprotocols AT osafunetetsuaki immunoelectronmicroscopymethodsandprotocols |