RNA interference has become a key method in the suppression of gene expression and the development of therapeutic agents, yet there is still the problem of delivery, stability, and the danger of off-target effects such as the silencing of unwanted genes and activation of innate immunity. In siRNA an...

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Bibliographic Details

Other Authors:	Sioud, Mouldy (Editor)
Format:	Electronic eBook
Language:	English
Published:	Totowa, NJ Humana Press 2009
Series:	Methods in Molecular Biology, Methods and Protocols 487
Subjects:	Life Sciences Biochemistry, general Human Genetics Gene Expression Life sciences Human genetics Gene expression Biochemistry RNS-Interferenz Aufsatzsammlung
Online Access:	UBR01 TUM01 Volltext
Summary:	RNA interference has become a key method in the suppression of gene expression and the development of therapeutic agents, yet there is still the problem of delivery, stability, and the danger of off-target effects such as the silencing of unwanted genes and activation of innate immunity. In siRNA and miRNA Gene Silencing: From Bench to Bedside, expert researchers explore the most recent advances in siRNA design, expression, delivery, in vivo imaging, and methods to minimize siRNA's unwanted effects and promote successful use in patients. As part of the highly successful Methods in Molecular Biology™ series, the chapters focus on their respective subjects with easy-to-use, up-to-date information, including several step-by-step laboratory protocols on topics such as new delivery formulations and strategies with promising applications in vitro and in vivo, validated therapeutic target genes, and components of miRNA function, biogenesis, and interference with virus infection. Comprehensive and cutting-edge, siRNA and miRNA Gene Silencing: From Bench to Bedside offers an excellent collection of chapters to aid all those with an interest in RNAi, gene regulation, and new therapies
Physical Description:	1 Online-Ressource (XIV, 472 p)
ISBN:	9781603275477
DOI:	10.1007/978-1-60327-547-7