Membrane Protein Structure and Function Characterization: Methods and Protocols
In this present volume, different approaches are detailed to produce membrane proteins, purify them, study their function, determine their structure, and model them in membrane. Since every membrane protein behaves mostly in a unique way /fashion, knowledge of guidelines and tricks may help to incre...
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| Weitere Verfasser: | |
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| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
New York, NY
Springer New York
2017
|
| Schriftenreihe: | Methods in Molecular Biology
1635 |
| Schlagworte: | |
| Online-Zugang: | UBR01 TUM01 Volltext |
| Zusammenfassung: | In this present volume, different approaches are detailed to produce membrane proteins, purify them, study their function, determine their structure, and model them in membrane. Since every membrane protein behaves mostly in a unique way /fashion, knowledge of guidelines and tricks may help to increase chances to express, purify and characterize a peculiar membrane protein. Production of correctly folded protein remains a challenge. Moreover, getting a functional and stable protein requires to optimize membrane mimicking environments that can be detergent or artificial membranes. In some cases, the finding of the correct ligand which will stabilize the desired conformation is needed. In other cases, stabilization can be obtained using specific antibodies. This volume also presents different techniques to analyze the functional status of membrane proteins. Written in the highly successful Methods in Molecular Biology series format, chapters in Membrane Protein Structure and Function Characterization: Methods and Protocols provide different techniques to analyze the functional and structural status of membrane proteins. Chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Membrane Protein Structure and Function Characterization: Methods and Protocols aims to ensure successful results in the further study of this vital field. |
| Beschreibung: | 1 Online-Ressource (XII, 419 p. 42 illus., 13 illus. in color) |
| ISBN: | 9781493971510 |
| DOI: | 10.1007/978-1-4939-7151-0 |
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Datensatz im Suchindex
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| dewey-full | 572.6 |
| dewey-hundreds | 500 - Natural sciences and mathematics |
| dewey-ones | 572 - Biochemistry |
| dewey-raw | 572.6 |
| dewey-search | 572.6 |
| dewey-sort | 3572.6 |
| dewey-tens | 570 - Biology |
| discipline | Biologie |
| doi_str_mv | 10.1007/978-1-4939-7151-0 |
| format | Electronic eBook |
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| id | DE-604.BV044951122 |
| illustrated | Not Illustrated |
| indexdate | 2024-07-10T08:05:35Z |
| institution | BVB |
| isbn | 9781493971510 |
| language | English |
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| spelling | Membrane Protein Structure and Function Characterization Methods and Protocols edited by Jean-Jacques Lacapere New York, NY Springer New York 2017 1 Online-Ressource (XII, 419 p. 42 illus., 13 illus. in color) txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology 1635 In this present volume, different approaches are detailed to produce membrane proteins, purify them, study their function, determine their structure, and model them in membrane. Since every membrane protein behaves mostly in a unique way /fashion, knowledge of guidelines and tricks may help to increase chances to express, purify and characterize a peculiar membrane protein. Production of correctly folded protein remains a challenge. Moreover, getting a functional and stable protein requires to optimize membrane mimicking environments that can be detergent or artificial membranes. In some cases, the finding of the correct ligand which will stabilize the desired conformation is needed. In other cases, stabilization can be obtained using specific antibodies. This volume also presents different techniques to analyze the functional status of membrane proteins. Written in the highly successful Methods in Molecular Biology series format, chapters in Membrane Protein Structure and Function Characterization: Methods and Protocols provide different techniques to analyze the functional and structural status of membrane proteins. Chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Membrane Protein Structure and Function Characterization: Methods and Protocols aims to ensure successful results in the further study of this vital field. Life Sciences Protein Science Life sciences Proteins Lacapere, Jean-Jacques edt Erscheint auch als Druck-Ausgabe 9781493971497 https://doi.org/10.1007/978-1-4939-7151-0 Verlag URL des Erstveröffentlichers Volltext |
| spellingShingle | Membrane Protein Structure and Function Characterization Methods and Protocols Life Sciences Protein Science Life sciences Proteins |
| title | Membrane Protein Structure and Function Characterization Methods and Protocols |
| title_auth | Membrane Protein Structure and Function Characterization Methods and Protocols |
| title_exact_search | Membrane Protein Structure and Function Characterization Methods and Protocols |
| title_full | Membrane Protein Structure and Function Characterization Methods and Protocols edited by Jean-Jacques Lacapere |
| title_fullStr | Membrane Protein Structure and Function Characterization Methods and Protocols edited by Jean-Jacques Lacapere |
| title_full_unstemmed | Membrane Protein Structure and Function Characterization Methods and Protocols edited by Jean-Jacques Lacapere |
| title_short | Membrane Protein Structure and Function Characterization |
| title_sort | membrane protein structure and function characterization methods and protocols |
| title_sub | Methods and Protocols |
| topic | Life Sciences Protein Science Life sciences Proteins |
| topic_facet | Life Sciences Protein Science Life sciences Proteins |
| url | https://doi.org/10.1007/978-1-4939-7151-0 |
| work_keys_str_mv | AT lacaperejeanjacques membraneproteinstructureandfunctioncharacterizationmethodsandprotocols |