Nitric Oxide: Methods and Protocols
The past two decades have seen an explosion in the number of research articles relating to both the physiological and pathological responses evoked by nitric oxide generation. Despite this, accurate quantification of nitric oxide in either in vitro or in vivo models remains challenging, due to the r...
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| Weitere Verfasser: | , |
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| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
Totowa, NJ
Humana Press
2011
|
| Ausgabe: | 1 |
| Schriftenreihe: | Methods in Molecular Biology, Methods and Protocols
704 |
| Schlagworte: | |
| Online-Zugang: | UBR01 TUM01 Volltext |
| Zusammenfassung: | The past two decades have seen an explosion in the number of research articles relating to both the physiological and pathological responses evoked by nitric oxide generation. Despite this, accurate quantification of nitric oxide in either in vitro or in vivo models remains challenging, due to the relatively unstable nature of the molecule. In Nitric Oxide: Methods and Protocols, experts in the field provide a detailed reference manual packed with step-by-step approaches to reproduce various complex protocols within your own laboratory. Divided into two parts, the volume first covers various disparate protocols relating to the direct detection and quantification of nitric oxide, including techniques which detail how to image real time in vivo generation of nitric oxide, quantify nitric oxide production in the rat brain, and the detect ultralow levels of nitric oxide in the pM range, and the book then continues with a section on techniques designed to either inhibit or enhance nitric oxide, with an aim to achieve therapeutic gain, featuring the inhibition of the nitric oxide synthase enzymes using viral, shRNA delivery systems to prevent cardiovascular dysfunction, peripheral neuropathy, and graft rejection. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and easy to use, Nitric Oxide: Methods and Protocols examines numerous complex methodologies detailed in each chapter and proves to be an invaluable resource for anyone with an interest in nitric oxide research |
| Beschreibung: | 1 Online-Ressource (XI, 226 p) |
| ISBN: | 9781617379642 |
| DOI: | 10.1007/978-1-61737-964-2 |
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| spelling | Nitric Oxide Methods and Protocols edited by Helen O. McCarthy, Jonathan A. Coulter 1 Totowa, NJ Humana Press 2011 1 Online-Ressource (XI, 226 p) txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology, Methods and Protocols 704 The past two decades have seen an explosion in the number of research articles relating to both the physiological and pathological responses evoked by nitric oxide generation. Despite this, accurate quantification of nitric oxide in either in vitro or in vivo models remains challenging, due to the relatively unstable nature of the molecule. In Nitric Oxide: Methods and Protocols, experts in the field provide a detailed reference manual packed with step-by-step approaches to reproduce various complex protocols within your own laboratory. Divided into two parts, the volume first covers various disparate protocols relating to the direct detection and quantification of nitric oxide, including techniques which detail how to image real time in vivo generation of nitric oxide, quantify nitric oxide production in the rat brain, and the detect ultralow levels of nitric oxide in the pM range, and the book then continues with a section on techniques designed to either inhibit or enhance nitric oxide, with an aim to achieve therapeutic gain, featuring the inhibition of the nitric oxide synthase enzymes using viral, shRNA delivery systems to prevent cardiovascular dysfunction, peripheral neuropathy, and graft rejection. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and easy to use, Nitric Oxide: Methods and Protocols examines numerous complex methodologies detailed in each chapter and proves to be an invaluable resource for anyone with an interest in nitric oxide research Life Sciences Biochemistry, general Life sciences Biochemistry Stickstoffoxide (DE-588)4057516-0 gnd rswk-swf Nachweis (DE-588)4115334-0 gnd rswk-swf Stickstoffstoffwechsel (DE-588)4138777-6 gnd rswk-swf Entstehung (DE-588)4156614-2 gnd rswk-swf Quantifizierung (DE-588)4176601-5 gnd rswk-swf Untersuchungsmethode (DE-588)4324907-3 gnd rswk-swf 1\p (DE-588)4143413-4 Aufsatzsammlung gnd-content Stickstoffstoffwechsel (DE-588)4138777-6 s Stickstoffoxide (DE-588)4057516-0 s Entstehung (DE-588)4156614-2 s Nachweis (DE-588)4115334-0 s Quantifizierung (DE-588)4176601-5 s Untersuchungsmethode (DE-588)4324907-3 s 2\p DE-604 McCarthy, Helen O. edt Coulter, Jonathan A. edt Erscheint auch als Druck-Ausgabe 9781617379635 https://doi.org/10.1007/978-1-61737-964-2 Verlag URL des Erstveröffentlichers Volltext 1\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk 2\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk |
| spellingShingle | Nitric Oxide Methods and Protocols Life Sciences Biochemistry, general Life sciences Biochemistry Stickstoffoxide (DE-588)4057516-0 gnd Nachweis (DE-588)4115334-0 gnd Stickstoffstoffwechsel (DE-588)4138777-6 gnd Entstehung (DE-588)4156614-2 gnd Quantifizierung (DE-588)4176601-5 gnd Untersuchungsmethode (DE-588)4324907-3 gnd |
| subject_GND | (DE-588)4057516-0 (DE-588)4115334-0 (DE-588)4138777-6 (DE-588)4156614-2 (DE-588)4176601-5 (DE-588)4324907-3 (DE-588)4143413-4 |
| title | Nitric Oxide Methods and Protocols |
| title_auth | Nitric Oxide Methods and Protocols |
| title_exact_search | Nitric Oxide Methods and Protocols |
| title_full | Nitric Oxide Methods and Protocols edited by Helen O. McCarthy, Jonathan A. Coulter |
| title_fullStr | Nitric Oxide Methods and Protocols edited by Helen O. McCarthy, Jonathan A. Coulter |
| title_full_unstemmed | Nitric Oxide Methods and Protocols edited by Helen O. McCarthy, Jonathan A. Coulter |
| title_short | Nitric Oxide |
| title_sort | nitric oxide methods and protocols |
| title_sub | Methods and Protocols |
| topic | Life Sciences Biochemistry, general Life sciences Biochemistry Stickstoffoxide (DE-588)4057516-0 gnd Nachweis (DE-588)4115334-0 gnd Stickstoffstoffwechsel (DE-588)4138777-6 gnd Entstehung (DE-588)4156614-2 gnd Quantifizierung (DE-588)4176601-5 gnd Untersuchungsmethode (DE-588)4324907-3 gnd |
| topic_facet | Life Sciences Biochemistry, general Life sciences Biochemistry Stickstoffoxide Nachweis Stickstoffstoffwechsel Entstehung Quantifizierung Untersuchungsmethode Aufsatzsammlung |
| url | https://doi.org/10.1007/978-1-61737-964-2 |
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