Basic Cell Culture Protocols:
In this fully revised edition of an established classic, expert researchers and clinicians describe in step-by-step detail updated techniques for the isolation and growth of the major primary cell types, such as kidney proximal tubule cells, hepatocytes, keratinocytes, and cardiomyocytes. The author...
Gespeichert in:
| Weitere Verfasser: | , |
|---|---|
| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
Totowa, NJ
Humana Press
2005
|
| Ausgabe: | Third Edition |
| Schriftenreihe: | Methods in Molecular Biology™
290 |
| Schlagworte: | |
| Online-Zugang: | UBR01 TUM01 URL des Erstveröffentlichers |
| Zusammenfassung: | In this fully revised edition of an established classic, expert researchers and clinicians describe in step-by-step detail updated techniques for the isolation and growth of the major primary cell types, such as kidney proximal tubule cells, hepatocytes, keratinocytes, and cardiomyocytes. The authors offer readily reproducible new methods for the differentiation of embryonic stem (ES) cells into various hematopoietic cell types, for fetal thymic organ culture, and for the isolation and culture of specialized cell types, such as mammary progenitor cells, skeletal muscle myofibers, mesenchymal cells, neural stem cells, hematopoietic cells, stromal cell lines, and endothelial cells. Additional chapters describe new techniques (leukocyte rolling, isolation of side population cells, and scalable production of ES-derived cells) and detail quality control methods for cell lines (detection and elimination of mycoplasma, DNA fingerprinting, and cytogenetic analysis). The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Up-to-date and highly practical, Basic Cell Culture Protocols, Third Edition, offers basic scientists and clinician-researchers powerful tools to isolate, culture, and characterize the promising specialized cell types in demand today |
| Beschreibung: | 1 Online-Ressource (XII, 371 p) |
| ISBN: | 9781592598380 |
| DOI: | 10.1385/1592598382 |
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| 520 | |a In this fully revised edition of an established classic, expert researchers and clinicians describe in step-by-step detail updated techniques for the isolation and growth of the major primary cell types, such as kidney proximal tubule cells, hepatocytes, keratinocytes, and cardiomyocytes. The authors offer readily reproducible new methods for the differentiation of embryonic stem (ES) cells into various hematopoietic cell types, for fetal thymic organ culture, and for the isolation and culture of specialized cell types, such as mammary progenitor cells, skeletal muscle myofibers, mesenchymal cells, neural stem cells, hematopoietic cells, stromal cell lines, and endothelial cells. Additional chapters describe new techniques (leukocyte rolling, isolation of side population cells, and scalable production of ES-derived cells) and detail quality control methods for cell lines (detection and elimination of mycoplasma, DNA fingerprinting, and cytogenetic analysis). The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Up-to-date and highly practical, Basic Cell Culture Protocols, Third Edition, offers basic scientists and clinician-researchers powerful tools to isolate, culture, and characterize the promising specialized cell types in demand today | ||
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Datensatz im Suchindex
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| spelling | Basic Cell Culture Protocols edited by Cheryl D. Helgason, Cindy L. Miller Third Edition Totowa, NJ Humana Press 2005 1 Online-Ressource (XII, 371 p) txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology™ 290 In this fully revised edition of an established classic, expert researchers and clinicians describe in step-by-step detail updated techniques for the isolation and growth of the major primary cell types, such as kidney proximal tubule cells, hepatocytes, keratinocytes, and cardiomyocytes. The authors offer readily reproducible new methods for the differentiation of embryonic stem (ES) cells into various hematopoietic cell types, for fetal thymic organ culture, and for the isolation and culture of specialized cell types, such as mammary progenitor cells, skeletal muscle myofibers, mesenchymal cells, neural stem cells, hematopoietic cells, stromal cell lines, and endothelial cells. Additional chapters describe new techniques (leukocyte rolling, isolation of side population cells, and scalable production of ES-derived cells) and detail quality control methods for cell lines (detection and elimination of mycoplasma, DNA fingerprinting, and cytogenetic analysis). The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Up-to-date and highly practical, Basic Cell Culture Protocols, Third Edition, offers basic scientists and clinician-researchers powerful tools to isolate, culture, and characterize the promising specialized cell types in demand today Life Sciences Cell Biology Life sciences Cell biology Gewebekultur (DE-588)4157245-2 gnd rswk-swf Zellkultur (DE-588)4067547-6 gnd rswk-swf 1\p (DE-588)4143413-4 Aufsatzsammlung gnd-content Zellkultur (DE-588)4067547-6 s DE-604 Gewebekultur (DE-588)4157245-2 s b DE-604 Helgason, Cheryl D. edt Miller, Cindy L. edt Erscheint auch als Druck-Ausgabe 9781588292841 https://doi.org/10.1385/1592598382 Verlag URL des Erstveröffentlichers Volltext 1\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk |
| spellingShingle | Basic Cell Culture Protocols Life Sciences Cell Biology Life sciences Cell biology Gewebekultur (DE-588)4157245-2 gnd Zellkultur (DE-588)4067547-6 gnd |
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| title | Basic Cell Culture Protocols |
| title_auth | Basic Cell Culture Protocols |
| title_exact_search | Basic Cell Culture Protocols |
| title_full | Basic Cell Culture Protocols edited by Cheryl D. Helgason, Cindy L. Miller |
| title_fullStr | Basic Cell Culture Protocols edited by Cheryl D. Helgason, Cindy L. Miller |
| title_full_unstemmed | Basic Cell Culture Protocols edited by Cheryl D. Helgason, Cindy L. Miller |
| title_short | Basic Cell Culture Protocols |
| title_sort | basic cell culture protocols |
| topic | Life Sciences Cell Biology Life sciences Cell biology Gewebekultur (DE-588)4157245-2 gnd Zellkultur (DE-588)4067547-6 gnd |
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