Metagenomics: Methods and Protocols
This second edition explores up-to-date tools in various function-based technologies currently used in metagenomics. The chapters in this book discuss all of the working steps involved in these technologies, such as: DNA isolation from soils and marine samples followed by the construction and screen...
Gespeichert in:
| Weitere Verfasser: | , |
|---|---|
| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
New York, NY
Springer New York
2017
|
| Ausgabe: | 2nd ed. 2017 |
| Schriftenreihe: | Methods in Molecular Biology
1539 |
| Schlagworte: | |
| Online-Zugang: | UBR01 TUM01 Volltext |
| Zusammenfassung: | This second edition explores up-to-date tools in various function-based technologies currently used in metagenomics. The chapters in this book discuss all of the working steps involved in these technologies, such as: DNA isolation from soils and marine samples followed by the construction and screening of libraries for diverse enzymes and biomolecules; current methods used to isolate DNA and construct large- and small-insert libraries from terrestrial and marine habitats; methods for establishing metagenome libraries in none-E.coli hosts; new molecular tools used for function-driven mining of metagenomic DNA; and screening protocols for a wide array of different genes encoding enzymes with relevance to biotechnology and ecology. Metagenomics: Methods and Protocols, Second Edition also provides detailed screening protocols for phosphatases, poly-hydroxyalkanoate, metabolism-related enzymes, stereoselective hydrolases, and microbial signals for the discovery of secondary metabolites. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Metagenomics: Methods and Protocols, Second Edition is a collection of up-to-date metagenome protocols and tools for the recovery of numerous major types of biocatalysts, and allows researchers to easily setup these screens in any microbiology laboratory. |
| Beschreibung: | 1 Online-Ressource (XI, 303 p. 48 illus., 24 illus. in color) |
| ISBN: | 9781493966912 |
| DOI: | 10.1007/978-1-4939-6691-2 |
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| edition | 2nd ed. 2017 |
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| spelling | Metagenomics Methods and Protocols edited by Wolfgang R. Streit, Rolf Daniel 2nd ed. 2017 New York, NY Springer New York 2017 1 Online-Ressource (XI, 303 p. 48 illus., 24 illus. in color) txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology 1539 This second edition explores up-to-date tools in various function-based technologies currently used in metagenomics. The chapters in this book discuss all of the working steps involved in these technologies, such as: DNA isolation from soils and marine samples followed by the construction and screening of libraries for diverse enzymes and biomolecules; current methods used to isolate DNA and construct large- and small-insert libraries from terrestrial and marine habitats; methods for establishing metagenome libraries in none-E.coli hosts; new molecular tools used for function-driven mining of metagenomic DNA; and screening protocols for a wide array of different genes encoding enzymes with relevance to biotechnology and ecology. Metagenomics: Methods and Protocols, Second Edition also provides detailed screening protocols for phosphatases, poly-hydroxyalkanoate, metabolism-related enzymes, stereoselective hydrolases, and microbial signals for the discovery of secondary metabolites. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Metagenomics: Methods and Protocols, Second Edition is a collection of up-to-date metagenome protocols and tools for the recovery of numerous major types of biocatalysts, and allows researchers to easily setup these screens in any microbiology laboratory. Biomedicine Human Genetics Medicine Human genetics Methode (DE-588)4038971-6 gnd rswk-swf Metagenom (DE-588)7635820-3 gnd rswk-swf 1\p (DE-588)4143413-4 Aufsatzsammlung gnd-content Metagenom (DE-588)7635820-3 s Methode (DE-588)4038971-6 s 2\p DE-604 Streit, Wolfgang R. edt Daniel, Rolf edt Erscheint auch als Druck-Ausgabe 9781493966899 https://doi.org/10.1007/978-1-4939-6691-2 Verlag URL des Erstveröffentlichers Volltext 1\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk 2\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk |
| spellingShingle | Metagenomics Methods and Protocols Biomedicine Human Genetics Medicine Human genetics Methode (DE-588)4038971-6 gnd Metagenom (DE-588)7635820-3 gnd |
| subject_GND | (DE-588)4038971-6 (DE-588)7635820-3 (DE-588)4143413-4 |
| title | Metagenomics Methods and Protocols |
| title_auth | Metagenomics Methods and Protocols |
| title_exact_search | Metagenomics Methods and Protocols |
| title_full | Metagenomics Methods and Protocols edited by Wolfgang R. Streit, Rolf Daniel |
| title_fullStr | Metagenomics Methods and Protocols edited by Wolfgang R. Streit, Rolf Daniel |
| title_full_unstemmed | Metagenomics Methods and Protocols edited by Wolfgang R. Streit, Rolf Daniel |
| title_short | Metagenomics |
| title_sort | metagenomics methods and protocols |
| title_sub | Methods and Protocols |
| topic | Biomedicine Human Genetics Medicine Human genetics Methode (DE-588)4038971-6 gnd Metagenom (DE-588)7635820-3 gnd |
| topic_facet | Biomedicine Human Genetics Medicine Human genetics Methode Metagenom Aufsatzsammlung |
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