Bacterial Artificial Chromosomes: Volume 1 Library Construction, Physical Mapping, and Sequencing
The ability to transfer and maintain DNA both within and between species is an essential skill in biotechnology and medicine. In Bacterial Artificial Chromosomes, expert investigators describe not only the classic methods, but also the many novel techniques they have perfected for the transfer of la...
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Weitere Verfasser: | , |
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Format: | Elektronisch E-Book |
Sprache: | English |
Veröffentlicht: |
Totowa, NJ
Humana Press
2004
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Schriftenreihe: | Methods in Molecular Biology™
255 |
Schlagworte: | |
Online-Zugang: | UBR01 TUM01 URL des Erstveröffentlichers |
Zusammenfassung: | The ability to transfer and maintain DNA both within and between species is an essential skill in biotechnology and medicine. In Bacterial Artificial Chromosomes, expert investigators describe not only the classic methods, but also the many novel techniques they have perfected for the transfer of large DNAs into the cells of both microbes and animals via large-insert recombinant DNAs. Volume 1: Library Construction, Physical Mapping, and Sequencing presents readily reproducible techniques for library construction, physical mapping, and sequencing. The laboratory protocols follow the successful Methods in Molecular Biology™ series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Besides protocols, each chapter includes scientific reviews, software tools, database resources, genome sequencing strategies, and illustrative case studies. An accompanying volume, Volume 2: Functional Studies, provides a wide variety of methods and applications for functional analysis of the DNA-transformed organisms. Comprehensive and cutting-edge, the two volumes of Bacterial Artificial Chromosomes provide a superlative collection of highly productive protocols that will prove useful to many bioscientists, including genome sequencers, geneticists, molecular biologists, and biochemists studying the structure and function of specific genomes |
Beschreibung: | 1 Online-Ressource (XX, 360 p. 71 illus) |
ISBN: | 9781592597529 |
DOI: | 10.1385/1592597521 |
Internformat
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520 | |a The ability to transfer and maintain DNA both within and between species is an essential skill in biotechnology and medicine. In Bacterial Artificial Chromosomes, expert investigators describe not only the classic methods, but also the many novel techniques they have perfected for the transfer of large DNAs into the cells of both microbes and animals via large-insert recombinant DNAs. Volume 1: Library Construction, Physical Mapping, and Sequencing presents readily reproducible techniques for library construction, physical mapping, and sequencing. The laboratory protocols follow the successful Methods in Molecular Biology™ series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Besides protocols, each chapter includes scientific reviews, software tools, database resources, genome sequencing strategies, and illustrative case studies. An accompanying volume, Volume 2: Functional Studies, provides a wide variety of methods and applications for functional analysis of the DNA-transformed organisms. Comprehensive and cutting-edge, the two volumes of Bacterial Artificial Chromosomes provide a superlative collection of highly productive protocols that will prove useful to many bioscientists, including genome sequencers, geneticists, molecular biologists, and biochemists studying the structure and function of specific genomes | ||
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Datensatz im Suchindex
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any_adam_object | |
author2 | Zhao, Shaying Stodolsky, Marvin |
author2_role | edt edt |
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dewey-hundreds | 500 - Natural sciences and mathematics |
dewey-ones | 579 - Microorganisms, fungi & algae |
dewey-raw | 579.3 |
dewey-search | 579.3 |
dewey-sort | 3579.3 |
dewey-tens | 570 - Biology |
discipline | Biologie |
doi_str_mv | 10.1385/1592597521 |
format | Electronic eBook |
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spelling | Bacterial Artificial Chromosomes Volume 1 Library Construction, Physical Mapping, and Sequencing edited by Shaying Zhao, Marvin Stodolsky Totowa, NJ Humana Press 2004 1 Online-Ressource (XX, 360 p. 71 illus) txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology™ 255 The ability to transfer and maintain DNA both within and between species is an essential skill in biotechnology and medicine. In Bacterial Artificial Chromosomes, expert investigators describe not only the classic methods, but also the many novel techniques they have perfected for the transfer of large DNAs into the cells of both microbes and animals via large-insert recombinant DNAs. Volume 1: Library Construction, Physical Mapping, and Sequencing presents readily reproducible techniques for library construction, physical mapping, and sequencing. The laboratory protocols follow the successful Methods in Molecular Biology™ series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Besides protocols, each chapter includes scientific reviews, software tools, database resources, genome sequencing strategies, and illustrative case studies. An accompanying volume, Volume 2: Functional Studies, provides a wide variety of methods and applications for functional analysis of the DNA-transformed organisms. Comprehensive and cutting-edge, the two volumes of Bacterial Artificial Chromosomes provide a superlative collection of highly productive protocols that will prove useful to many bioscientists, including genome sequencers, geneticists, molecular biologists, and biochemists studying the structure and function of specific genomes Life Sciences Bacteriology Microbiology Biochemistry, general Life sciences Biochemistry Bakterien (DE-588)4004296-0 gnd rswk-swf Labortechnik (DE-588)4123602-6 gnd rswk-swf Künstliches Chromosom (DE-588)4767094-0 gnd rswk-swf Bakterien (DE-588)4004296-0 s Künstliches Chromosom (DE-588)4767094-0 s Labortechnik (DE-588)4123602-6 s b DE-604 Zhao, Shaying edt Stodolsky, Marvin edt Erscheint auch als Druck-Ausgabe 9780896039889 https://doi.org/10.1385/1592597521 Verlag URL des Erstveröffentlichers Volltext |
spellingShingle | Bacterial Artificial Chromosomes Volume 1 Library Construction, Physical Mapping, and Sequencing Life Sciences Bacteriology Microbiology Biochemistry, general Life sciences Biochemistry Bakterien (DE-588)4004296-0 gnd Labortechnik (DE-588)4123602-6 gnd Künstliches Chromosom (DE-588)4767094-0 gnd |
subject_GND | (DE-588)4004296-0 (DE-588)4123602-6 (DE-588)4767094-0 |
title | Bacterial Artificial Chromosomes Volume 1 Library Construction, Physical Mapping, and Sequencing |
title_auth | Bacterial Artificial Chromosomes Volume 1 Library Construction, Physical Mapping, and Sequencing |
title_exact_search | Bacterial Artificial Chromosomes Volume 1 Library Construction, Physical Mapping, and Sequencing |
title_full | Bacterial Artificial Chromosomes Volume 1 Library Construction, Physical Mapping, and Sequencing edited by Shaying Zhao, Marvin Stodolsky |
title_fullStr | Bacterial Artificial Chromosomes Volume 1 Library Construction, Physical Mapping, and Sequencing edited by Shaying Zhao, Marvin Stodolsky |
title_full_unstemmed | Bacterial Artificial Chromosomes Volume 1 Library Construction, Physical Mapping, and Sequencing edited by Shaying Zhao, Marvin Stodolsky |
title_short | Bacterial Artificial Chromosomes |
title_sort | bacterial artificial chromosomes volume 1 library construction physical mapping and sequencing |
title_sub | Volume 1 Library Construction, Physical Mapping, and Sequencing |
topic | Life Sciences Bacteriology Microbiology Biochemistry, general Life sciences Biochemistry Bakterien (DE-588)4004296-0 gnd Labortechnik (DE-588)4123602-6 gnd Künstliches Chromosom (DE-588)4767094-0 gnd |
topic_facet | Life Sciences Bacteriology Microbiology Biochemistry, general Life sciences Biochemistry Bakterien Labortechnik Künstliches Chromosom |
url | https://doi.org/10.1385/1592597521 |
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