High-Resolution Imaging of Cellular Proteins: Methods and Protocols

This volume presents authoritative and cutting-edge methods and protocols focusing on three tool boxes covering the increasingly diverse methodologies used to image selected proteins and to investigate their function by light and electron microscopy. The first tool box includes the development of a...

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Weitere Verfasser: Schwartzbach, Steven D. (HerausgeberIn), Skalli, Omar (HerausgeberIn), Schikorski, Thomas (HerausgeberIn)
Format: Elektronisch E-Book
Sprache:English
Veröffentlicht: New York, NY Springer New York 2016
Schriftenreihe:Methods in Molecular Biology 1474
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Zusammenfassung:This volume presents authoritative and cutting-edge methods and protocols focusing on three tool boxes covering the increasingly diverse methodologies used to image selected proteins and to investigate their function by light and electron microscopy. The first tool box includes the development of a wide range of molecular and immunological probes to target specific proteins. The second details the use of these probes for high resolution fluorescence microscopy and the third focuses on applications for transmission and scanning electron microscopy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls thus ensuring successful results in the further study of this vital field
Beschreibung:1 Online-Ressource (XIII, 366 p. 45 illus., 21 illus. in color)
ISBN:9781493963522
DOI:10.1007/978-1-4939-6352-2

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