Differential Display Methods and Protocols:
Carrying on the high standards of the first edition of Differential Display Methods, Peng Liang et al. have based their second edition on a new mathematical model of differential display (DD) that takes advantage of automation, as well as digital data acquisition and analysis. These well-versed auth...
Gespeichert in:
| Weitere Verfasser: | , , |
|---|---|
| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
Totowa, NJ
Humana Press
2006
|
| Ausgabe: | Second Edition |
| Schriftenreihe: | Methods in Molecular Biology
317 |
| Schlagworte: | |
| Online-Zugang: | UBR01 TUM01 Volltext |
| Zusammenfassung: | Carrying on the high standards of the first edition of Differential Display Methods, Peng Liang et al. have based their second edition on a new mathematical model of differential display (DD) that takes advantage of automation, as well as digital data acquisition and analysis. These well-versed authors explain and highlight all the recent methodological refinements, including automated liquid handling of hundreds of DD PCR reaction setups combined with capillary electrophoresis, a prototype computer program to automatically allow positive band identification from a fluorescence differential display image, and restriction fragment-based DD screenings that can link any cDNA fragment directly to a given gene once the sequence information of all transcripts becomes available. Other improvements discussed are combining DD and DNA microarrays by reducing the complexity of cDNA probes while increasing the sensitivity of detection, and a DD approach to detect prokaryotic mRNA expression. The authors also demonstrate the power of DD technology with a collection of outstanding examples of DD applications and detailed experimental procedures. The elegant studies described here have led to the discovery of many important genes involved in viral infection, Prion disease, cancer, ovulation, circadian clock, floral color, transcription repression gene silencing, mRNA polymorphism, and protein-RNA interaction. State-of-the-art and highly practical, Differential Display Methods, Second Edition offers gene hunters the possibility of genome-wide comprehensive DD screening, as well as a proven road map for any successful "gene fishing" expedition |
| Beschreibung: | 1 Online-Ressource (XIV, 320 p. 132 illus., 1 illus. in color) |
| ISBN: | 9781592599684 |
| DOI: | 10.1385/1592599680 |
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| 520 | |a Carrying on the high standards of the first edition of Differential Display Methods, Peng Liang et al. have based their second edition on a new mathematical model of differential display (DD) that takes advantage of automation, as well as digital data acquisition and analysis. These well-versed authors explain and highlight all the recent methodological refinements, including automated liquid handling of hundreds of DD PCR reaction setups combined with capillary electrophoresis, a prototype computer program to automatically allow positive band identification from a fluorescence differential display image, and restriction fragment-based DD screenings that can link any cDNA fragment directly to a given gene once the sequence information of all transcripts becomes available. Other improvements discussed are combining DD and DNA microarrays by reducing the complexity of cDNA probes while increasing the sensitivity of detection, and a DD approach to detect prokaryotic mRNA expression. The authors also demonstrate the power of DD technology with a collection of outstanding examples of DD applications and detailed experimental procedures. The elegant studies described here have led to the discovery of many important genes involved in viral infection, Prion disease, cancer, ovulation, circadian clock, floral color, transcription repression gene silencing, mRNA polymorphism, and protein-RNA interaction. State-of-the-art and highly practical, Differential Display Methods, Second Edition offers gene hunters the possibility of genome-wide comprehensive DD screening, as well as a proven road map for any successful "gene fishing" expedition | ||
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Datensatz im Suchindex
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| any_adam_object | |
| author2 | Liang, Peng Meade, Jonathan D. Pardee, Arthur B. |
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| discipline | Biologie Chemie |
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| spelling | Differential Display Methods and Protocols edited by Peng Liang, Jonathan D. Meade, Arthur B. Pardee Second Edition Totowa, NJ Humana Press 2006 1 Online-Ressource (XIV, 320 p. 132 illus., 1 illus. in color) txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology 317 Carrying on the high standards of the first edition of Differential Display Methods, Peng Liang et al. have based their second edition on a new mathematical model of differential display (DD) that takes advantage of automation, as well as digital data acquisition and analysis. These well-versed authors explain and highlight all the recent methodological refinements, including automated liquid handling of hundreds of DD PCR reaction setups combined with capillary electrophoresis, a prototype computer program to automatically allow positive band identification from a fluorescence differential display image, and restriction fragment-based DD screenings that can link any cDNA fragment directly to a given gene once the sequence information of all transcripts becomes available. Other improvements discussed are combining DD and DNA microarrays by reducing the complexity of cDNA probes while increasing the sensitivity of detection, and a DD approach to detect prokaryotic mRNA expression. The authors also demonstrate the power of DD technology with a collection of outstanding examples of DD applications and detailed experimental procedures. The elegant studies described here have led to the discovery of many important genes involved in viral infection, Prion disease, cancer, ovulation, circadian clock, floral color, transcription repression gene silencing, mRNA polymorphism, and protein-RNA interaction. State-of-the-art and highly practical, Differential Display Methods, Second Edition offers gene hunters the possibility of genome-wide comprehensive DD screening, as well as a proven road map for any successful "gene fishing" expedition Life Sciences Cell Biology Life sciences Cell biology Fingerprint-Verfahren (DE-588)4231579-7 gnd rswk-swf Genklonierung (DE-588)4123275-6 gnd rswk-swf Methode (DE-588)4038971-6 gnd rswk-swf 1\p (DE-588)4143413-4 Aufsatzsammlung gnd-content Fingerprint-Verfahren (DE-588)4231579-7 s Methode (DE-588)4038971-6 s DE-604 Genklonierung (DE-588)4123275-6 s Liang, Peng edt Meade, Jonathan D. edt Pardee, Arthur B. edt Erscheint auch als Druck-Ausgabe 9781588293381 https://doi.org/10.1385/1592599680 Verlag URL des Erstveröffentlichers Volltext 1\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk |
| spellingShingle | Differential Display Methods and Protocols Life Sciences Cell Biology Life sciences Cell biology Fingerprint-Verfahren (DE-588)4231579-7 gnd Genklonierung (DE-588)4123275-6 gnd Methode (DE-588)4038971-6 gnd |
| subject_GND | (DE-588)4231579-7 (DE-588)4123275-6 (DE-588)4038971-6 (DE-588)4143413-4 |
| title | Differential Display Methods and Protocols |
| title_auth | Differential Display Methods and Protocols |
| title_exact_search | Differential Display Methods and Protocols |
| title_full | Differential Display Methods and Protocols edited by Peng Liang, Jonathan D. Meade, Arthur B. Pardee |
| title_fullStr | Differential Display Methods and Protocols edited by Peng Liang, Jonathan D. Meade, Arthur B. Pardee |
| title_full_unstemmed | Differential Display Methods and Protocols edited by Peng Liang, Jonathan D. Meade, Arthur B. Pardee |
| title_short | Differential Display Methods and Protocols |
| title_sort | differential display methods and protocols |
| topic | Life Sciences Cell Biology Life sciences Cell biology Fingerprint-Verfahren (DE-588)4231579-7 gnd Genklonierung (DE-588)4123275-6 gnd Methode (DE-588)4038971-6 gnd |
| topic_facet | Life Sciences Cell Biology Life sciences Cell biology Fingerprint-Verfahren Genklonierung Methode Aufsatzsammlung |
| url | https://doi.org/10.1385/1592599680 |
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