Chromatin Immunoprecipitation Assays: Methods and Protocols
Over the past twenty years, the development of chromatin immunoprecipitation, or ChIP, assays has immensely enhanced the biological significance of the multifaceted DNA-binding proteins. In Chromatin Immunoprecipitation Assays: Methods and Protocols, researchers deeply involved in the development an...
Gespeichert in:
Weitere Verfasser: | |
---|---|
Format: | Elektronisch E-Book |
Sprache: | English |
Veröffentlicht: |
Totowa, NJ
Humana Press
2009
|
Schriftenreihe: | Methods in Molecular Biology, Methods and Protocols
567 |
Schlagworte: | |
Online-Zugang: | UBR01 TUM01 Volltext |
Zusammenfassung: | Over the past twenty years, the development of chromatin immunoprecipitation, or ChIP, assays has immensely enhanced the biological significance of the multifaceted DNA-binding proteins. In Chromatin Immunoprecipitation Assays: Methods and Protocols, researchers deeply involved in the development and improvement of the field provide cutting-edge protocols devoted to the most recent progress in ChIP and related subjects. The thorough chapters involve topics such as the characterization of ChIP antibodies, ChIP methods for small cell numbers, fast ChIP protocols, assays adapted to various species and cell types, as well as several strategies for the analysis of genome-wide datasets, while also extending a bit beyond ChIP assays to cover immunoprecipitation-based DNA methylation analyses, determination of spatial chromatin organization of large genomic regions, and RNA immunoprecipitation. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain brief introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easy-to-use, Chromatin Immunoprecipitation Assays: Methods and Protocols is a timely guide for all those who wish to further the development of this powerful tool and continue delving into the incredible complexity of the cell’s biology |
Beschreibung: | 1 Online-Ressource (X, 268 p. 47 illus) |
ISBN: | 9781603274142 |
DOI: | 10.1007/978-1-60327-414-2 |
Internformat
MARC
LEADER | 00000nmm a2200000zcb4500 | ||
---|---|---|---|
001 | BV044951004 | ||
003 | DE-604 | ||
005 | 00000000000000.0 | ||
007 | cr|uuu---uuuuu | ||
008 | 180517s2009 |||| o||u| ||||||eng d | ||
020 | |a 9781603274142 |9 978-1-60327-414-2 | ||
024 | 7 | |a 10.1007/978-1-60327-414-2 |2 doi | |
035 | |a (ZDB-2-PRO)978-1-60327-414-2 | ||
035 | |a (OCoLC)712421429 | ||
035 | |a (DE-599)BVBBV044951004 | ||
040 | |a DE-604 |b ger |e rda | ||
041 | 0 | |a eng | |
049 | |a DE-355 |a DE-91 | ||
082 | 0 | |a 572 |2 23 | |
084 | |a WC 4460 |0 (DE-625)148102: |2 rvk | ||
245 | 1 | 0 | |a Chromatin Immunoprecipitation Assays |b Methods and Protocols |c edited by Philippe Collas |
264 | 1 | |a Totowa, NJ |b Humana Press |c 2009 | |
300 | |a 1 Online-Ressource (X, 268 p. 47 illus) | ||
336 | |b txt |2 rdacontent | ||
337 | |b c |2 rdamedia | ||
338 | |b cr |2 rdacarrier | ||
490 | 0 | |a Methods in Molecular Biology, Methods and Protocols |v 567 | |
520 | |a Over the past twenty years, the development of chromatin immunoprecipitation, or ChIP, assays has immensely enhanced the biological significance of the multifaceted DNA-binding proteins. In Chromatin Immunoprecipitation Assays: Methods and Protocols, researchers deeply involved in the development and improvement of the field provide cutting-edge protocols devoted to the most recent progress in ChIP and related subjects. The thorough chapters involve topics such as the characterization of ChIP antibodies, ChIP methods for small cell numbers, fast ChIP protocols, assays adapted to various species and cell types, as well as several strategies for the analysis of genome-wide datasets, while also extending a bit beyond ChIP assays to cover immunoprecipitation-based DNA methylation analyses, determination of spatial chromatin organization of large genomic regions, and RNA immunoprecipitation. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain brief introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easy-to-use, Chromatin Immunoprecipitation Assays: Methods and Protocols is a timely guide for all those who wish to further the development of this powerful tool and continue delving into the incredible complexity of the cell’s biology | ||
650 | 4 | |a Life Sciences | |
650 | 4 | |a Biochemistry, general | |
650 | 4 | |a Human Genetics | |
650 | 4 | |a Microarrays | |
650 | 4 | |a Life sciences | |
650 | 4 | |a Human genetics | |
650 | 4 | |a Microarrays | |
650 | 4 | |a Biochemistry | |
650 | 0 | 7 | |a Chromatin |0 (DE-588)4010152-6 |2 gnd |9 rswk-swf |
650 | 0 | 7 | |a DNS-Chip |0 (DE-588)4721796-0 |2 gnd |9 rswk-swf |
655 | 7 | |8 1\p |0 (DE-588)4143413-4 |a Aufsatzsammlung |2 gnd-content | |
689 | 0 | 0 | |a Chromatin |0 (DE-588)4010152-6 |D s |
689 | 0 | 1 | |a DNS-Chip |0 (DE-588)4721796-0 |D s |
689 | 0 | |8 2\p |5 DE-604 | |
700 | 1 | |a Collas, Philippe |4 edt | |
776 | 0 | 8 | |i Erscheint auch als |n Druck-Ausgabe |z 9781603274135 |
856 | 4 | 0 | |u https://doi.org/10.1007/978-1-60327-414-2 |x Verlag |z URL des Erstveröffentlichers |3 Volltext |
912 | |a ZDB-2-PRO | ||
999 | |a oai:aleph.bib-bvb.de:BVB01-030343763 | ||
883 | 1 | |8 1\p |a cgwrk |d 20201028 |q DE-101 |u https://d-nb.info/provenance/plan#cgwrk | |
883 | 1 | |8 2\p |a cgwrk |d 20201028 |q DE-101 |u https://d-nb.info/provenance/plan#cgwrk | |
966 | e | |u https://doi.org/10.1007/978-1-60327-414-2 |l UBR01 |p ZDB-2-PRO |x Verlag |3 Volltext | |
966 | e | |u https://doi.org/10.1007/978-1-60327-414-2 |l TUM01 |p ZDB-2-PRO |x Verlag |3 Volltext |
Datensatz im Suchindex
_version_ | 1804178539107319808 |
---|---|
any_adam_object | |
author2 | Collas, Philippe |
author2_role | edt |
author2_variant | p c pc |
author_facet | Collas, Philippe |
building | Verbundindex |
bvnumber | BV044951004 |
classification_rvk | WC 4460 |
collection | ZDB-2-PRO |
ctrlnum | (ZDB-2-PRO)978-1-60327-414-2 (OCoLC)712421429 (DE-599)BVBBV044951004 |
dewey-full | 572 |
dewey-hundreds | 500 - Natural sciences and mathematics |
dewey-ones | 572 - Biochemistry |
dewey-raw | 572 |
dewey-search | 572 |
dewey-sort | 3572 |
dewey-tens | 570 - Biology |
discipline | Biologie |
doi_str_mv | 10.1007/978-1-60327-414-2 |
format | Electronic eBook |
fullrecord | <?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>03601nmm a2200577zcb4500</leader><controlfield tag="001">BV044951004</controlfield><controlfield tag="003">DE-604</controlfield><controlfield tag="005">00000000000000.0</controlfield><controlfield tag="007">cr|uuu---uuuuu</controlfield><controlfield tag="008">180517s2009 |||| o||u| ||||||eng d</controlfield><datafield tag="020" ind1=" " ind2=" "><subfield code="a">9781603274142</subfield><subfield code="9">978-1-60327-414-2</subfield></datafield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1007/978-1-60327-414-2</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(ZDB-2-PRO)978-1-60327-414-2</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(OCoLC)712421429</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)BVBBV044951004</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-604</subfield><subfield code="b">ger</subfield><subfield code="e">rda</subfield></datafield><datafield tag="041" ind1="0" ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="049" ind1=" " ind2=" "><subfield code="a">DE-355</subfield><subfield code="a">DE-91</subfield></datafield><datafield tag="082" ind1="0" ind2=" "><subfield code="a">572</subfield><subfield code="2">23</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">WC 4460</subfield><subfield code="0">(DE-625)148102:</subfield><subfield code="2">rvk</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Chromatin Immunoprecipitation Assays</subfield><subfield code="b">Methods and Protocols</subfield><subfield code="c">edited by Philippe Collas</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="a">Totowa, NJ</subfield><subfield code="b">Humana Press</subfield><subfield code="c">2009</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">1 Online-Ressource (X, 268 p. 47 illus)</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="490" ind1="0" ind2=" "><subfield code="a">Methods in Molecular Biology, Methods and Protocols</subfield><subfield code="v">567</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Over the past twenty years, the development of chromatin immunoprecipitation, or ChIP, assays has immensely enhanced the biological significance of the multifaceted DNA-binding proteins. In Chromatin Immunoprecipitation Assays: Methods and Protocols, researchers deeply involved in the development and improvement of the field provide cutting-edge protocols devoted to the most recent progress in ChIP and related subjects. The thorough chapters involve topics such as the characterization of ChIP antibodies, ChIP methods for small cell numbers, fast ChIP protocols, assays adapted to various species and cell types, as well as several strategies for the analysis of genome-wide datasets, while also extending a bit beyond ChIP assays to cover immunoprecipitation-based DNA methylation analyses, determination of spatial chromatin organization of large genomic regions, and RNA immunoprecipitation. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain brief introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easy-to-use, Chromatin Immunoprecipitation Assays: Methods and Protocols is a timely guide for all those who wish to further the development of this powerful tool and continue delving into the incredible complexity of the cell’s biology</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Life Sciences</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Biochemistry, general</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Human Genetics</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Microarrays</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Life sciences</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Human genetics</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Microarrays</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Biochemistry</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Chromatin</subfield><subfield code="0">(DE-588)4010152-6</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">DNS-Chip</subfield><subfield code="0">(DE-588)4721796-0</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="655" ind1=" " ind2="7"><subfield code="8">1\p</subfield><subfield code="0">(DE-588)4143413-4</subfield><subfield code="a">Aufsatzsammlung</subfield><subfield code="2">gnd-content</subfield></datafield><datafield tag="689" ind1="0" ind2="0"><subfield code="a">Chromatin</subfield><subfield code="0">(DE-588)4010152-6</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2="1"><subfield code="a">DNS-Chip</subfield><subfield code="0">(DE-588)4721796-0</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2=" "><subfield code="8">2\p</subfield><subfield code="5">DE-604</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Collas, Philippe</subfield><subfield code="4">edt</subfield></datafield><datafield tag="776" ind1="0" ind2="8"><subfield code="i">Erscheint auch als</subfield><subfield code="n">Druck-Ausgabe</subfield><subfield code="z">9781603274135</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://doi.org/10.1007/978-1-60327-414-2</subfield><subfield code="x">Verlag</subfield><subfield code="z">URL des Erstveröffentlichers</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">ZDB-2-PRO</subfield></datafield><datafield tag="999" ind1=" " ind2=" "><subfield code="a">oai:aleph.bib-bvb.de:BVB01-030343763</subfield></datafield><datafield tag="883" ind1="1" ind2=" "><subfield code="8">1\p</subfield><subfield code="a">cgwrk</subfield><subfield code="d">20201028</subfield><subfield code="q">DE-101</subfield><subfield code="u">https://d-nb.info/provenance/plan#cgwrk</subfield></datafield><datafield tag="883" ind1="1" ind2=" "><subfield code="8">2\p</subfield><subfield code="a">cgwrk</subfield><subfield code="d">20201028</subfield><subfield code="q">DE-101</subfield><subfield code="u">https://d-nb.info/provenance/plan#cgwrk</subfield></datafield><datafield tag="966" ind1="e" ind2=" "><subfield code="u">https://doi.org/10.1007/978-1-60327-414-2</subfield><subfield code="l">UBR01</subfield><subfield code="p">ZDB-2-PRO</subfield><subfield code="x">Verlag</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="966" ind1="e" ind2=" "><subfield code="u">https://doi.org/10.1007/978-1-60327-414-2</subfield><subfield code="l">TUM01</subfield><subfield code="p">ZDB-2-PRO</subfield><subfield code="x">Verlag</subfield><subfield code="3">Volltext</subfield></datafield></record></collection> |
genre | 1\p (DE-588)4143413-4 Aufsatzsammlung gnd-content |
genre_facet | Aufsatzsammlung |
id | DE-604.BV044951004 |
illustrated | Not Illustrated |
indexdate | 2024-07-10T08:05:34Z |
institution | BVB |
isbn | 9781603274142 |
language | English |
oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-030343763 |
oclc_num | 712421429 |
open_access_boolean | |
owner | DE-355 DE-BY-UBR DE-91 DE-BY-TUM |
owner_facet | DE-355 DE-BY-UBR DE-91 DE-BY-TUM |
physical | 1 Online-Ressource (X, 268 p. 47 illus) |
psigel | ZDB-2-PRO |
publishDate | 2009 |
publishDateSearch | 2009 |
publishDateSort | 2009 |
publisher | Humana Press |
record_format | marc |
series2 | Methods in Molecular Biology, Methods and Protocols |
spelling | Chromatin Immunoprecipitation Assays Methods and Protocols edited by Philippe Collas Totowa, NJ Humana Press 2009 1 Online-Ressource (X, 268 p. 47 illus) txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology, Methods and Protocols 567 Over the past twenty years, the development of chromatin immunoprecipitation, or ChIP, assays has immensely enhanced the biological significance of the multifaceted DNA-binding proteins. In Chromatin Immunoprecipitation Assays: Methods and Protocols, researchers deeply involved in the development and improvement of the field provide cutting-edge protocols devoted to the most recent progress in ChIP and related subjects. The thorough chapters involve topics such as the characterization of ChIP antibodies, ChIP methods for small cell numbers, fast ChIP protocols, assays adapted to various species and cell types, as well as several strategies for the analysis of genome-wide datasets, while also extending a bit beyond ChIP assays to cover immunoprecipitation-based DNA methylation analyses, determination of spatial chromatin organization of large genomic regions, and RNA immunoprecipitation. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain brief introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easy-to-use, Chromatin Immunoprecipitation Assays: Methods and Protocols is a timely guide for all those who wish to further the development of this powerful tool and continue delving into the incredible complexity of the cell’s biology Life Sciences Biochemistry, general Human Genetics Microarrays Life sciences Human genetics Biochemistry Chromatin (DE-588)4010152-6 gnd rswk-swf DNS-Chip (DE-588)4721796-0 gnd rswk-swf 1\p (DE-588)4143413-4 Aufsatzsammlung gnd-content Chromatin (DE-588)4010152-6 s DNS-Chip (DE-588)4721796-0 s 2\p DE-604 Collas, Philippe edt Erscheint auch als Druck-Ausgabe 9781603274135 https://doi.org/10.1007/978-1-60327-414-2 Verlag URL des Erstveröffentlichers Volltext 1\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk 2\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk |
spellingShingle | Chromatin Immunoprecipitation Assays Methods and Protocols Life Sciences Biochemistry, general Human Genetics Microarrays Life sciences Human genetics Biochemistry Chromatin (DE-588)4010152-6 gnd DNS-Chip (DE-588)4721796-0 gnd |
subject_GND | (DE-588)4010152-6 (DE-588)4721796-0 (DE-588)4143413-4 |
title | Chromatin Immunoprecipitation Assays Methods and Protocols |
title_auth | Chromatin Immunoprecipitation Assays Methods and Protocols |
title_exact_search | Chromatin Immunoprecipitation Assays Methods and Protocols |
title_full | Chromatin Immunoprecipitation Assays Methods and Protocols edited by Philippe Collas |
title_fullStr | Chromatin Immunoprecipitation Assays Methods and Protocols edited by Philippe Collas |
title_full_unstemmed | Chromatin Immunoprecipitation Assays Methods and Protocols edited by Philippe Collas |
title_short | Chromatin Immunoprecipitation Assays |
title_sort | chromatin immunoprecipitation assays methods and protocols |
title_sub | Methods and Protocols |
topic | Life Sciences Biochemistry, general Human Genetics Microarrays Life sciences Human genetics Biochemistry Chromatin (DE-588)4010152-6 gnd DNS-Chip (DE-588)4721796-0 gnd |
topic_facet | Life Sciences Biochemistry, general Human Genetics Microarrays Life sciences Human genetics Biochemistry Chromatin DNS-Chip Aufsatzsammlung |
url | https://doi.org/10.1007/978-1-60327-414-2 |
work_keys_str_mv | AT collasphilippe chromatinimmunoprecipitationassaysmethodsandprotocols |