Nuclear Reprogramming: Methods and Protocols, Second Edition includes not only classic methods to perform nuclear transfer in different species but also several techniques to assess the early and late development of the reconstructed embryos, at the cellular, molecular, and epigenetic level. Over th...

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Bibliographische Detailangaben

Weitere Verfasser:	Beaujean, Nathalie (HerausgeberIn), Jammes, Hélène (HerausgeberIn), Jouneau, Alice (HerausgeberIn)
Format:	Elektronisch E-Book
Sprache:	English
Veröffentlicht:	New York, NY Springer New York 2015
Ausgabe:	2nd ed. 2015
Schriftenreihe:	Methods in Molecular Biology, Methods and Protocols 1222
Schlagworte:	Life Sciences Cell Biology Genetic Engineering Life sciences Genetic engineering Cell biology Zellkern Physiologie Kerntransplantation Aufsatzsammlung
Online-Zugang:	UBR01 TUM01 Volltext
Zusammenfassung:	Nuclear Reprogramming: Methods and Protocols, Second Edition includes not only classic methods to perform nuclear transfer in different species but also several techniques to assess the early and late development of the reconstructed embryos, at the cellular, molecular, and epigenetic level. Over the past several years, many technical improvements have been made to improve somatic cell nuclear transfer (SCNT) efficiency, all of which are reflected in the detailed chapters of this fully revised collection. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.   Authoritative and up-to-date, Nuclear Reprogramming: Methods and Protocols, Second Edition will be of interest not only to cloners but also to researchers concerned with studying the development of embryos
Beschreibung:	1 Online-Ressource (XI, 285 p. 68 illus., 43 illus. in color)
ISBN:	9781493915941
DOI:	10.1007/978-1-4939-1594-1