Pichia Protocols:
The Pichia pastoris expression system has rapidly become one of the key protein expression systems used in commercial, academic, and government laboratories today. In Pichia Protocols, David Higgins and James Cregg compile for the first time an all-inclusive collection of powerful experimental proce...
Gespeichert in:
| Weitere Verfasser: | , |
|---|---|
| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
Totowa, NJ
Humana Press
1998
|
| Schriftenreihe: | Methods in Molecular Biology™
103 |
| Schlagworte: | |
| Online-Zugang: | UBR01 Volltext |
| Zusammenfassung: | The Pichia pastoris expression system has rapidly become one of the key protein expression systems used in commercial, academic, and government laboratories today. In Pichia Protocols, David Higgins and James Cregg compile for the first time an all-inclusive collection of powerful experimental procedures to manipulate Pichia pastoris for gene expression purposes. The methods-developed by world-class researchers in their own laboratories-contain detailed descriptions of available expression strains, as well as vectors, and step-by-step instructions for the construction of expression strains and for their use in the production of foreign proteins from shake-flask or fermentor cultures. General procedures for the purification of foreign proteins are also provided, along with protocols for analyzing foreign protein products for glycosylation and subcellular location. Pichia Protocols captures the collective expertise of the international P. pastoris scientific community in close detail. It conveys a breadth of experience that will permit any researcher in molecular biology to quickly construct an expression strain designed to produce a desired foreign protein, and to use this state-of-the-art molecular and cellular biological system successfully in every area of biological research today |
| Beschreibung: | 1 Online-Ressource (XI, 270 p) |
| ISBN: | 9781592595785 |
| DOI: | 10.1385/0896034216 |
Internformat
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| 490 | 0 | |a Methods in Molecular Biology™ |v 103 | |
| 520 | |a The Pichia pastoris expression system has rapidly become one of the key protein expression systems used in commercial, academic, and government laboratories today. In Pichia Protocols, David Higgins and James Cregg compile for the first time an all-inclusive collection of powerful experimental procedures to manipulate Pichia pastoris for gene expression purposes. The methods-developed by world-class researchers in their own laboratories-contain detailed descriptions of available expression strains, as well as vectors, and step-by-step instructions for the construction of expression strains and for their use in the production of foreign proteins from shake-flask or fermentor cultures. General procedures for the purification of foreign proteins are also provided, along with protocols for analyzing foreign protein products for glycosylation and subcellular location. Pichia Protocols captures the collective expertise of the international P. pastoris scientific community in close detail. It conveys a breadth of experience that will permit any researcher in molecular biology to quickly construct an expression strain designed to produce a desired foreign protein, and to use this state-of-the-art molecular and cellular biological system successfully in every area of biological research today | ||
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Datensatz im Suchindex
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| dewey-full | 571.6 |
| dewey-hundreds | 500 - Natural sciences and mathematics |
| dewey-ones | 571 - Physiology & related subjects |
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| dewey-tens | 570 - Biology |
| discipline | Biologie |
| doi_str_mv | 10.1385/0896034216 |
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| indexdate | 2024-07-10T08:05:34Z |
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| isbn | 9781592595785 |
| language | English |
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| series2 | Methods in Molecular Biology™ |
| spelling | Pichia Protocols edited by David R. Higgins, James M. Cregg Totowa, NJ Humana Press 1998 1 Online-Ressource (XI, 270 p) txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology™ 103 The Pichia pastoris expression system has rapidly become one of the key protein expression systems used in commercial, academic, and government laboratories today. In Pichia Protocols, David Higgins and James Cregg compile for the first time an all-inclusive collection of powerful experimental procedures to manipulate Pichia pastoris for gene expression purposes. The methods-developed by world-class researchers in their own laboratories-contain detailed descriptions of available expression strains, as well as vectors, and step-by-step instructions for the construction of expression strains and for their use in the production of foreign proteins from shake-flask or fermentor cultures. General procedures for the purification of foreign proteins are also provided, along with protocols for analyzing foreign protein products for glycosylation and subcellular location. Pichia Protocols captures the collective expertise of the international P. pastoris scientific community in close detail. It conveys a breadth of experience that will permit any researcher in molecular biology to quickly construct an expression strain designed to produce a desired foreign protein, and to use this state-of-the-art molecular and cellular biological system successfully in every area of biological research today Life Sciences Cell Biology Life sciences Cell biology Pichia pastoris (DE-588)4407211-9 gnd rswk-swf Vektor Genetik (DE-588)4279246-0 gnd rswk-swf Pichia (DE-588)4273120-3 gnd rswk-swf Biotechnologie (DE-588)4069491-4 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Pichia pastoris (DE-588)4407211-9 s Vektor Genetik (DE-588)4279246-0 s DE-604 Pichia (DE-588)4273120-3 s Biotechnologie (DE-588)4069491-4 s Higgins, David R. edt Cregg, James M. edt Erscheint auch als Druck-Ausgabe 978-0-89603-421-1 https://doi.org/10.1385/0896034216 Verlag URL des Erstveröffentlichers Volltext |
| spellingShingle | Pichia Protocols Life Sciences Cell Biology Life sciences Cell biology Pichia pastoris (DE-588)4407211-9 gnd Vektor Genetik (DE-588)4279246-0 gnd Pichia (DE-588)4273120-3 gnd Biotechnologie (DE-588)4069491-4 gnd |
| subject_GND | (DE-588)4407211-9 (DE-588)4279246-0 (DE-588)4273120-3 (DE-588)4069491-4 (DE-588)4143413-4 |
| title | Pichia Protocols |
| title_auth | Pichia Protocols |
| title_exact_search | Pichia Protocols |
| title_full | Pichia Protocols edited by David R. Higgins, James M. Cregg |
| title_fullStr | Pichia Protocols edited by David R. Higgins, James M. Cregg |
| title_full_unstemmed | Pichia Protocols edited by David R. Higgins, James M. Cregg |
| title_short | Pichia Protocols |
| title_sort | pichia protocols |
| topic | Life Sciences Cell Biology Life sciences Cell biology Pichia pastoris (DE-588)4407211-9 gnd Vektor Genetik (DE-588)4279246-0 gnd Pichia (DE-588)4273120-3 gnd Biotechnologie (DE-588)4069491-4 gnd |
| topic_facet | Life Sciences Cell Biology Life sciences Cell biology Pichia pastoris Vektor Genetik Pichia Biotechnologie Aufsatzsammlung |
| url | https://doi.org/10.1385/0896034216 |
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