Therapeutic Oligonucleotides: Methods and Protocols
The field of oligonucleotide therapeutics research is ripe with the prospect of new discoveries. In Therapeutic Oligonucleotides: Methods and Protocols, a selection of established and emerging methods for the application of oligonucleotides as therapeutics are presented, all providing the tools need...
Gespeichert in:
| Weitere Verfasser: | |
|---|---|
| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
Totowa, NJ
Humana Press
2011
|
| Schriftenreihe: | Methods in Molecular Biology, Methods and Protocols
764 |
| Schlagworte: | |
| Online-Zugang: | UBR01 TUM01 Volltext |
| Zusammenfassung: | The field of oligonucleotide therapeutics research is ripe with the prospect of new discoveries. In Therapeutic Oligonucleotides: Methods and Protocols, a selection of established and emerging methods for the application of oligonucleotides as therapeutics are presented, all providing the tools needed to inspire great changes in the field. Divided into twenty-one chapters, this detailed volume meticulously describes vital protocols for optimizing and improving cell uptake, such as photochemical internalization, modified cell penetrating peptides, antibody conjugates, and nanoparticles. Other chapters address quantitation of RNA therapeutics in cells, assaying gene knockdown, selecting the best target site and synthesis of various modified oligonucleotides. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Therapeutic Oligonucleotides: Methods and Protocols serves as a timely resource for both professionals and novices pursuing research in this exciting and pioneering field |
| Beschreibung: | 1 Online-Ressource (XI, 340 p. 70 illus) |
| ISBN: | 9781617791888 |
| DOI: | 10.1007/978-1-61779-188-8 |
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| spelling | Therapeutic Oligonucleotides Methods and Protocols edited by John Goodchild Totowa, NJ Humana Press 2011 1 Online-Ressource (XI, 340 p. 70 illus) txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology, Methods and Protocols 764 The field of oligonucleotide therapeutics research is ripe with the prospect of new discoveries. In Therapeutic Oligonucleotides: Methods and Protocols, a selection of established and emerging methods for the application of oligonucleotides as therapeutics are presented, all providing the tools needed to inspire great changes in the field. Divided into twenty-one chapters, this detailed volume meticulously describes vital protocols for optimizing and improving cell uptake, such as photochemical internalization, modified cell penetrating peptides, antibody conjugates, and nanoparticles. Other chapters address quantitation of RNA therapeutics in cells, assaying gene knockdown, selecting the best target site and synthesis of various modified oligonucleotides. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Therapeutic Oligonucleotides: Methods and Protocols serves as a timely resource for both professionals and novices pursuing research in this exciting and pioneering field Life Sciences Biochemistry, general Nucleic Acid Chemistry Life sciences Biochemistry Nucleic acids Labortechnik (DE-588)4123602-6 gnd rswk-swf Oligonucleotide (DE-588)4133456-5 gnd rswk-swf Pharmakotherapie (DE-588)4076066-2 gnd rswk-swf Oligonucleotide (DE-588)4133456-5 s Pharmakotherapie (DE-588)4076066-2 s Labortechnik (DE-588)4123602-6 s b DE-604 Goodchild, John edt Erscheint auch als Druck-Ausgabe 9781617791871 https://doi.org/10.1007/978-1-61779-188-8 Verlag URL des Erstveröffentlichers Volltext |
| spellingShingle | Therapeutic Oligonucleotides Methods and Protocols Life Sciences Biochemistry, general Nucleic Acid Chemistry Life sciences Biochemistry Nucleic acids Labortechnik (DE-588)4123602-6 gnd Oligonucleotide (DE-588)4133456-5 gnd Pharmakotherapie (DE-588)4076066-2 gnd |
| subject_GND | (DE-588)4123602-6 (DE-588)4133456-5 (DE-588)4076066-2 |
| title | Therapeutic Oligonucleotides Methods and Protocols |
| title_auth | Therapeutic Oligonucleotides Methods and Protocols |
| title_exact_search | Therapeutic Oligonucleotides Methods and Protocols |
| title_full | Therapeutic Oligonucleotides Methods and Protocols edited by John Goodchild |
| title_fullStr | Therapeutic Oligonucleotides Methods and Protocols edited by John Goodchild |
| title_full_unstemmed | Therapeutic Oligonucleotides Methods and Protocols edited by John Goodchild |
| title_short | Therapeutic Oligonucleotides |
| title_sort | therapeutic oligonucleotides methods and protocols |
| title_sub | Methods and Protocols |
| topic | Life Sciences Biochemistry, general Nucleic Acid Chemistry Life sciences Biochemistry Nucleic acids Labortechnik (DE-588)4123602-6 gnd Oligonucleotide (DE-588)4133456-5 gnd Pharmakotherapie (DE-588)4076066-2 gnd |
| topic_facet | Life Sciences Biochemistry, general Nucleic Acid Chemistry Life sciences Biochemistry Nucleic acids Labortechnik Oligonucleotide Pharmakotherapie |
| url | https://doi.org/10.1007/978-1-61779-188-8 |
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