Homing Endonucleases: Methods and Protocols
Homing Endonucleases: Methods and Protocols aims at providing molecular biologists with a comprehensive resource to identify and characterize homing endonucleases from genomic sequence, to deduce the biological basis of binding and cleavage specificity, as well as to provide protocols to redesign en...
Gespeichert in:
| Weitere Verfasser: | |
|---|---|
| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
Totowa, NJ
Humana Press
2014
|
| Schriftenreihe: | Methods in Molecular Biology, Methods and Protocols
1123 |
| Schlagworte: | |
| Online-Zugang: | UBR01 TUM01 Volltext |
| Zusammenfassung: | Homing Endonucleases: Methods and Protocols aims at providing molecular biologists with a comprehensive resource to identify and characterize homing endonucleases from genomic sequence, to deduce the biological basis of binding and cleavage specificity, as well as to provide protocols to redesign endonuclease target specificity for genome-editing applications. Engineering of designer homing endonucleases has set the stage for genome editing of complex eukaryotic genomes with a broad range of potential applications including targeted gene knockouts in model organisms and gene therapy in humans, making this book a valuable resource for future research. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Homing Endonucleases: Methods and Protocols serves as a key reference for all labs studying site-specific DNA endonucleases |
| Beschreibung: | 1 Online-Ressource (X, 284 p. 60 illus., 39 illus. in color) |
| ISBN: | 9781627039680 |
| DOI: | 10.1007/978-1-62703-968-0 |
Internformat
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Datensatz im Suchindex
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| spelling | Homing Endonucleases Methods and Protocols edited by David R. Edgell Totowa, NJ Humana Press 2014 1 Online-Ressource (X, 284 p. 60 illus., 39 illus. in color) txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology, Methods and Protocols 1123 Homing Endonucleases: Methods and Protocols aims at providing molecular biologists with a comprehensive resource to identify and characterize homing endonucleases from genomic sequence, to deduce the biological basis of binding and cleavage specificity, as well as to provide protocols to redesign endonuclease target specificity for genome-editing applications. Engineering of designer homing endonucleases has set the stage for genome editing of complex eukaryotic genomes with a broad range of potential applications including targeted gene knockouts in model organisms and gene therapy in humans, making this book a valuable resource for future research. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Homing Endonucleases: Methods and Protocols serves as a key reference for all labs studying site-specific DNA endonucleases Biomedicine Human Genetics Genetic Engineering Medicine Human genetics Genetic engineering Labortechnik (DE-588)4123602-6 gnd rswk-swf Endonucleasen (DE-588)4152179-1 gnd rswk-swf 1\p (DE-588)4143413-4 Aufsatzsammlung gnd-content Endonucleasen (DE-588)4152179-1 s Labortechnik (DE-588)4123602-6 s DE-604 Edgell, David R. edt Erscheint auch als Druck-Ausgabe 9781627039673 https://doi.org/10.1007/978-1-62703-968-0 Verlag URL des Erstveröffentlichers Volltext 1\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk |
| spellingShingle | Homing Endonucleases Methods and Protocols Biomedicine Human Genetics Genetic Engineering Medicine Human genetics Genetic engineering Labortechnik (DE-588)4123602-6 gnd Endonucleasen (DE-588)4152179-1 gnd |
| subject_GND | (DE-588)4123602-6 (DE-588)4152179-1 (DE-588)4143413-4 |
| title | Homing Endonucleases Methods and Protocols |
| title_auth | Homing Endonucleases Methods and Protocols |
| title_exact_search | Homing Endonucleases Methods and Protocols |
| title_full | Homing Endonucleases Methods and Protocols edited by David R. Edgell |
| title_fullStr | Homing Endonucleases Methods and Protocols edited by David R. Edgell |
| title_full_unstemmed | Homing Endonucleases Methods and Protocols edited by David R. Edgell |
| title_short | Homing Endonucleases |
| title_sort | homing endonucleases methods and protocols |
| title_sub | Methods and Protocols |
| topic | Biomedicine Human Genetics Genetic Engineering Medicine Human genetics Genetic engineering Labortechnik (DE-588)4123602-6 gnd Endonucleasen (DE-588)4152179-1 gnd |
| topic_facet | Biomedicine Human Genetics Genetic Engineering Medicine Human genetics Genetic engineering Labortechnik Endonucleasen Aufsatzsammlung |
| url | https://doi.org/10.1007/978-1-62703-968-0 |
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