2-D Proteome Analysis Protocols:
In 2-D Gel Proteome Analysis Protocols, Andrew Link and his expert collaborators take today's researchers step-by-step through the complete process of doing proteomics. With easy-to-follow instructions, complete with many helpful hints and explanations, leading investigators and pioneers in the...
Gespeichert in:
| Weitere Verfasser: | |
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| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
Totowa, NJ
Humana Press
1999
|
| Schriftenreihe: | Methods in Molecular Biology
112 |
| Schlagworte: | |
| Online-Zugang: | UBR01 Volltext |
| Zusammenfassung: | In 2-D Gel Proteome Analysis Protocols, Andrew Link and his expert collaborators take today's researchers step-by-step through the complete process of doing proteomics. With easy-to-follow instructions, complete with many helpful hints and explanations, leading investigators and pioneers in the field show how to make protein extracts, reproducibly run them on a 2-D gels, detect them, analyze the data, and precisely identify each protein. The book covers the latest methods of using carrier ampholytes in the 1st dimension, casting and running immobilized pH gradient 2-D gels, MALDI-TOF-based peptide mapping, automated tandem mass spectrometry, and nanoelectrospray ionization technology. For the 2nd dimension, there are methods for running flatbed or vertical gels and for protein detection using autoradiography, and Coomassie, silver, and reversible metal-chelate stains. The book is a perfect complement to the genome sequencing project for answering biological questions. 2-D Gel Proteome Analysis Protocols is the most complete guide for using proteomics to answer biological questions. Whether it is a question of global protein analysis or evaluating a cell's response to internal or external stimuli, the advanced methods described here will enable today's researchers better to understand how cells work and open new possibilities for drug discovery |
| Beschreibung: | 1 Online-Ressource (XVII, 601 p) |
| ISBN: | 9781592595846 |
| DOI: | 10.1385/1592595847 |
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Datensatz im Suchindex
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| dewey-full | 572 572/.6 |
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| discipline | Chemie / Pharmazie Biologie |
| doi_str_mv | 10.1385/1592595847 |
| format | Electronic eBook |
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| illustrated | Not Illustrated |
| indexdate | 2024-07-10T08:05:34Z |
| institution | BVB |
| isbn | 9781592595846 |
| language | English |
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| spelling | 2-D Proteome Analysis Protocols edited by Andrew J. Link Totowa, NJ Humana Press 1999 1 Online-Ressource (XVII, 601 p) txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology 112 In 2-D Gel Proteome Analysis Protocols, Andrew Link and his expert collaborators take today's researchers step-by-step through the complete process of doing proteomics. With easy-to-follow instructions, complete with many helpful hints and explanations, leading investigators and pioneers in the field show how to make protein extracts, reproducibly run them on a 2-D gels, detect them, analyze the data, and precisely identify each protein. The book covers the latest methods of using carrier ampholytes in the 1st dimension, casting and running immobilized pH gradient 2-D gels, MALDI-TOF-based peptide mapping, automated tandem mass spectrometry, and nanoelectrospray ionization technology. For the 2nd dimension, there are methods for running flatbed or vertical gels and for protein detection using autoradiography, and Coomassie, silver, and reversible metal-chelate stains. The book is a perfect complement to the genome sequencing project for answering biological questions. 2-D Gel Proteome Analysis Protocols is the most complete guide for using proteomics to answer biological questions. Whether it is a question of global protein analysis or evaluating a cell's response to internal or external stimuli, the advanced methods described here will enable today's researchers better to understand how cells work and open new possibilities for drug discovery Life Sciences Biochemistry, general Life sciences Biochemistry Zweidimensionale Elektrophorese (DE-588)4221393-9 gnd rswk-swf Genexpression (DE-588)4020136-3 gnd rswk-swf Proteine (DE-588)4076388-2 gnd rswk-swf 1\p (DE-588)4143413-4 Aufsatzsammlung gnd-content Proteine (DE-588)4076388-2 s Genexpression (DE-588)4020136-3 s Zweidimensionale Elektrophorese (DE-588)4221393-9 s DE-604 Link, Andrew J. edt Erscheint auch als Druck-Ausgabe 9780896035249 https://doi.org/10.1385/1592595847 Verlag URL des Erstveröffentlichers Volltext 1\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk |
| spellingShingle | 2-D Proteome Analysis Protocols Life Sciences Biochemistry, general Life sciences Biochemistry Zweidimensionale Elektrophorese (DE-588)4221393-9 gnd Genexpression (DE-588)4020136-3 gnd Proteine (DE-588)4076388-2 gnd |
| subject_GND | (DE-588)4221393-9 (DE-588)4020136-3 (DE-588)4076388-2 (DE-588)4143413-4 |
| title | 2-D Proteome Analysis Protocols |
| title_auth | 2-D Proteome Analysis Protocols |
| title_exact_search | 2-D Proteome Analysis Protocols |
| title_full | 2-D Proteome Analysis Protocols edited by Andrew J. Link |
| title_fullStr | 2-D Proteome Analysis Protocols edited by Andrew J. Link |
| title_full_unstemmed | 2-D Proteome Analysis Protocols edited by Andrew J. Link |
| title_short | 2-D Proteome Analysis Protocols |
| title_sort | 2 d proteome analysis protocols |
| topic | Life Sciences Biochemistry, general Life sciences Biochemistry Zweidimensionale Elektrophorese (DE-588)4221393-9 gnd Genexpression (DE-588)4020136-3 gnd Proteine (DE-588)4076388-2 gnd |
| topic_facet | Life Sciences Biochemistry, general Life sciences Biochemistry Zweidimensionale Elektrophorese Genexpression Proteine Aufsatzsammlung |
| url | https://doi.org/10.1385/1592595847 |
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