Membrane Protein Protocols: Expression, Purification, and Characterization
Recent developments in molecular biology and biochemistry have made it possible to determine the three-dimensional structure of membrane proteins and so come to a full understanding of their function. In Membrane Protein Protocols: Expression, Purification, and Characterization, researchers at major...
Gespeichert in:
| Weitere Verfasser: | |
|---|---|
| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
Totowa, NJ
Humana Press
2003
|
| Schriftenreihe: | Methods in Molecular Biology
228 |
| Schlagworte: | |
| Online-Zugang: | UBR01 TUM01 Volltext |
| Zusammenfassung: | Recent developments in molecular biology and biochemistry have made it possible to determine the three-dimensional structure of membrane proteins and so come to a full understanding of their function. In Membrane Protein Protocols: Expression, Purification, and Characterization, researchers at major universities and research centers around the world describe in detail the key techniques that have proven successful in the study of receptors and transport proteins. The book provides examples of how different membrane proteins can be overexpressed in both prokaryotic and eukaryotic expression systems, how natural and overexpressed proteins can be solubilized from their host membranes, and how the solubilized protein can be purified in active form. Each protocol contains step-by-step instructions to ensure success, troubleshooting advice, lists of reagents, and tips on avoiding pitfalls. Timely and highly practical, Membrane Protein Protocols: Expression, Purification, and Characterization offers investigators proven methods to determine the three-dimensional structure of membrane receptors and transport proteins, as well as proven practical help in the discovery of new clinical targets for the design of novel pharmaceutical agents useful in treating cancer, bacterial and viral infection, and many metabolic diseases |
| Beschreibung: | 1 Online-Ressource (XXII, 334 p) |
| ISBN: | 9781592594009 |
| DOI: | 10.1385/159259400X |
Internformat
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| spelling | Membrane Protein Protocols Expression, Purification, and Characterization edited by Barry S. Selinsky Totowa, NJ Humana Press 2003 1 Online-Ressource (XXII, 334 p) txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology 228 Recent developments in molecular biology and biochemistry have made it possible to determine the three-dimensional structure of membrane proteins and so come to a full understanding of their function. In Membrane Protein Protocols: Expression, Purification, and Characterization, researchers at major universities and research centers around the world describe in detail the key techniques that have proven successful in the study of receptors and transport proteins. The book provides examples of how different membrane proteins can be overexpressed in both prokaryotic and eukaryotic expression systems, how natural and overexpressed proteins can be solubilized from their host membranes, and how the solubilized protein can be purified in active form. Each protocol contains step-by-step instructions to ensure success, troubleshooting advice, lists of reagents, and tips on avoiding pitfalls. Timely and highly practical, Membrane Protein Protocols: Expression, Purification, and Characterization offers investigators proven methods to determine the three-dimensional structure of membrane receptors and transport proteins, as well as proven practical help in the discovery of new clinical targets for the design of novel pharmaceutical agents useful in treating cancer, bacterial and viral infection, and many metabolic diseases Life Sciences Biochemistry, general Life sciences Biochemistry Untersuchungsmethode (DE-588)4324907-3 gnd rswk-swf Membranproteine (DE-588)4130026-9 gnd rswk-swf 1\p (DE-588)4143413-4 Aufsatzsammlung gnd-content Membranproteine (DE-588)4130026-9 s Untersuchungsmethode (DE-588)4324907-3 s DE-604 Selinsky, Barry S. edt Erscheint auch als Druck-Ausgabe 9781588291240 https://doi.org/10.1385/159259400X Verlag URL des Erstveröffentlichers Volltext 1\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk |
| spellingShingle | Membrane Protein Protocols Expression, Purification, and Characterization Life Sciences Biochemistry, general Life sciences Biochemistry Untersuchungsmethode (DE-588)4324907-3 gnd Membranproteine (DE-588)4130026-9 gnd |
| subject_GND | (DE-588)4324907-3 (DE-588)4130026-9 (DE-588)4143413-4 |
| title | Membrane Protein Protocols Expression, Purification, and Characterization |
| title_auth | Membrane Protein Protocols Expression, Purification, and Characterization |
| title_exact_search | Membrane Protein Protocols Expression, Purification, and Characterization |
| title_full | Membrane Protein Protocols Expression, Purification, and Characterization edited by Barry S. Selinsky |
| title_fullStr | Membrane Protein Protocols Expression, Purification, and Characterization edited by Barry S. Selinsky |
| title_full_unstemmed | Membrane Protein Protocols Expression, Purification, and Characterization edited by Barry S. Selinsky |
| title_short | Membrane Protein Protocols |
| title_sort | membrane protein protocols expression purification and characterization |
| title_sub | Expression, Purification, and Characterization |
| topic | Life Sciences Biochemistry, general Life sciences Biochemistry Untersuchungsmethode (DE-588)4324907-3 gnd Membranproteine (DE-588)4130026-9 gnd |
| topic_facet | Life Sciences Biochemistry, general Life sciences Biochemistry Untersuchungsmethode Membranproteine Aufsatzsammlung |
| url | https://doi.org/10.1385/159259400X |
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