Quantitative PCR Protocols:
In Quantitative PCR Protocols, Bernd Kochanowski and Udo Reischl assemble a significant collection of established and novel methods for the successful quantitation of nucleic acids. Each method has been refined and tested by its developer and proven to work in such problems as the analysis of eukary...
Gespeichert in:
| Weitere Verfasser: | , |
|---|---|
| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
Totowa, NJ
Humana Press
1999
|
| Schriftenreihe: | Methods in Molecular Medicine™
26 |
| Schlagworte: | |
| Online-Zugang: | UBR01 Volltext |
| Zusammenfassung: | In Quantitative PCR Protocols, Bernd Kochanowski and Udo Reischl assemble a significant collection of established and novel methods for the successful quantitation of nucleic acids. Each method has been refined and tested by its developer and proven to work in such problems as the analysis of eukaryotic gene expression, the quantitation of viral loads in clinical specimens, reporter gene expression, and quantitative oncogene analysis. Particular emphasis is placed on the underlying principles of the design of competitive or noncompetitive standards, as well as on the optimization of the amplification process. In important cases several methods are given for the same problem so that readers may set up test systems tailored to their specific practical needs. Quantitative PCR Protocols provides a collection of detailed methods that offers comprehensive and up-to-date information on how to control PCR to obtain reliable quantitation. With its step-by-step instructions researchers can really master the complexities of PCR and address otherwise inaccessible biological and diagnostic questions |
| Beschreibung: | 1 Online-Ressource (320 p) |
| ISBN: | 9781592592623 |
| DOI: | 10.1385/0896035182 |
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| 520 | |a In Quantitative PCR Protocols, Bernd Kochanowski and Udo Reischl assemble a significant collection of established and novel methods for the successful quantitation of nucleic acids. Each method has been refined and tested by its developer and proven to work in such problems as the analysis of eukaryotic gene expression, the quantitation of viral loads in clinical specimens, reporter gene expression, and quantitative oncogene analysis. Particular emphasis is placed on the underlying principles of the design of competitive or noncompetitive standards, as well as on the optimization of the amplification process. In important cases several methods are given for the same problem so that readers may set up test systems tailored to their specific practical needs. Quantitative PCR Protocols provides a collection of detailed methods that offers comprehensive and up-to-date information on how to control PCR to obtain reliable quantitation. With its step-by-step instructions researchers can really master the complexities of PCR and address otherwise inaccessible biological and diagnostic questions | ||
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| discipline | Biologie |
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| spelling | Quantitative PCR Protocols edited by Bernd Kochanowski, Udo Reischl Totowa, NJ Humana Press 1999 1 Online-Ressource (320 p) txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Medicine™ 26 In Quantitative PCR Protocols, Bernd Kochanowski and Udo Reischl assemble a significant collection of established and novel methods for the successful quantitation of nucleic acids. Each method has been refined and tested by its developer and proven to work in such problems as the analysis of eukaryotic gene expression, the quantitation of viral loads in clinical specimens, reporter gene expression, and quantitative oncogene analysis. Particular emphasis is placed on the underlying principles of the design of competitive or noncompetitive standards, as well as on the optimization of the amplification process. In important cases several methods are given for the same problem so that readers may set up test systems tailored to their specific practical needs. Quantitative PCR Protocols provides a collection of detailed methods that offers comprehensive and up-to-date information on how to control PCR to obtain reliable quantitation. With its step-by-step instructions researchers can really master the complexities of PCR and address otherwise inaccessible biological and diagnostic questions Life Sciences Cell Biology Life sciences Cell biology Polymerase-Kettenreaktion (DE-588)4256726-9 gnd rswk-swf Gen (DE-588)4128987-0 gnd rswk-swf Quantitative Analyse (DE-588)4125311-5 gnd rswk-swf 1\p (DE-588)4143413-4 Aufsatzsammlung gnd-content Gen (DE-588)4128987-0 s Quantitative Analyse (DE-588)4125311-5 s Polymerase-Kettenreaktion (DE-588)4256726-9 s 2\p DE-604 Kochanowski, Bernd edt Reischl, Udo edt Erscheint auch als Druck-Ausgabe 9780896035188 https://doi.org/10.1385/0896035182 Verlag URL des Erstveröffentlichers Volltext 1\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk 2\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk |
| spellingShingle | Quantitative PCR Protocols Life Sciences Cell Biology Life sciences Cell biology Polymerase-Kettenreaktion (DE-588)4256726-9 gnd Gen (DE-588)4128987-0 gnd Quantitative Analyse (DE-588)4125311-5 gnd |
| subject_GND | (DE-588)4256726-9 (DE-588)4128987-0 (DE-588)4125311-5 (DE-588)4143413-4 |
| title | Quantitative PCR Protocols |
| title_auth | Quantitative PCR Protocols |
| title_exact_search | Quantitative PCR Protocols |
| title_full | Quantitative PCR Protocols edited by Bernd Kochanowski, Udo Reischl |
| title_fullStr | Quantitative PCR Protocols edited by Bernd Kochanowski, Udo Reischl |
| title_full_unstemmed | Quantitative PCR Protocols edited by Bernd Kochanowski, Udo Reischl |
| title_short | Quantitative PCR Protocols |
| title_sort | quantitative pcr protocols |
| topic | Life Sciences Cell Biology Life sciences Cell biology Polymerase-Kettenreaktion (DE-588)4256726-9 gnd Gen (DE-588)4128987-0 gnd Quantitative Analyse (DE-588)4125311-5 gnd |
| topic_facet | Life Sciences Cell Biology Life sciences Cell biology Polymerase-Kettenreaktion Gen Quantitative Analyse Aufsatzsammlung |
| url | https://doi.org/10.1385/0896035182 |
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