Natural Killer Cell Protocols: Cellular and Molecular Methods
In Natural Killer Cell Protocols: Cellular and Molecular Methods, Kerry S. Campbell and Marco Colonna have assembled a comprehensive collection of readily reproducible methods designed to study natural killer (NK) cells from the broadest variety of viewpoints. These include not only classic techniqu...
Gespeichert in:
| Weitere Verfasser: | , |
|---|---|
| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
Totowa, NJ
Humana Press
2000
|
| Schriftenreihe: | Methods in Molecular Biology
121 |
| Schlagworte: | |
| Online-Zugang: | UBR01 TUM01 Volltext |
| Zusammenfassung: | In Natural Killer Cell Protocols: Cellular and Molecular Methods, Kerry S. Campbell and Marco Colonna have assembled a comprehensive collection of readily reproducible methods designed to study natural killer (NK) cells from the broadest variety of viewpoints. These include not only classic techniques, but also new approaches to standard methods, newly evolved techniques that have become valuable for specific applications, and unique models for manipulating and studying NK cells. Among the advanced methods covered are those for in vitro transendothelial migration, in vivo detection of cells migrating into tumors, immunofluorescence staining of intracellular cytokines, and in vitro NK cell development. Valuable techniques for specific applications include vaccinia virus protein expression, soluble KIR-Fc fusions for HLA class I binding assays, calcium mobilization in cell conjugates, and identification of heterodimeric receptor complexes using cDNA library expression cloning. No less important are accounts of such classic methods as hybrid resistance, ADCC, viral defense, target cell cytotoxicity assays, cloning and culturing, tumor immunotherapy, and generation of HLA class I transfected target cells. Natural Killer Cell Protocols: Cellular and Molecular Methods offers immunologists, cancer researchers, virologists, and cell biologists today's most comprehensive collection of both established and cutting-edge techniques, methods that will contribute significantly to advancing our understanding of this fascinating and critically important class of cells |
| Beschreibung: | 1 Online-Ressource (XI, 398 p) |
| ISBN: | 9781592590445 |
| DOI: | 10.1385/1592590446 |
Internformat
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| 520 | |a In Natural Killer Cell Protocols: Cellular and Molecular Methods, Kerry S. Campbell and Marco Colonna have assembled a comprehensive collection of readily reproducible methods designed to study natural killer (NK) cells from the broadest variety of viewpoints. These include not only classic techniques, but also new approaches to standard methods, newly evolved techniques that have become valuable for specific applications, and unique models for manipulating and studying NK cells. Among the advanced methods covered are those for in vitro transendothelial migration, in vivo detection of cells migrating into tumors, immunofluorescence staining of intracellular cytokines, and in vitro NK cell development. Valuable techniques for specific applications include vaccinia virus protein expression, soluble KIR-Fc fusions for HLA class I binding assays, calcium mobilization in cell conjugates, and identification of heterodimeric receptor complexes using cDNA library expression cloning. No less important are accounts of such classic methods as hybrid resistance, ADCC, viral defense, target cell cytotoxicity assays, cloning and culturing, tumor immunotherapy, and generation of HLA class I transfected target cells. Natural Killer Cell Protocols: Cellular and Molecular Methods offers immunologists, cancer researchers, virologists, and cell biologists today's most comprehensive collection of both established and cutting-edge techniques, methods that will contribute significantly to advancing our understanding of this fascinating and critically important class of cells | ||
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| spelling | Natural Killer Cell Protocols Cellular and Molecular Methods edited by Kerry S. Campbell, Marco Colonna Totowa, NJ Humana Press 2000 1 Online-Ressource (XI, 398 p) txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology 121 In Natural Killer Cell Protocols: Cellular and Molecular Methods, Kerry S. Campbell and Marco Colonna have assembled a comprehensive collection of readily reproducible methods designed to study natural killer (NK) cells from the broadest variety of viewpoints. These include not only classic techniques, but also new approaches to standard methods, newly evolved techniques that have become valuable for specific applications, and unique models for manipulating and studying NK cells. Among the advanced methods covered are those for in vitro transendothelial migration, in vivo detection of cells migrating into tumors, immunofluorescence staining of intracellular cytokines, and in vitro NK cell development. Valuable techniques for specific applications include vaccinia virus protein expression, soluble KIR-Fc fusions for HLA class I binding assays, calcium mobilization in cell conjugates, and identification of heterodimeric receptor complexes using cDNA library expression cloning. No less important are accounts of such classic methods as hybrid resistance, ADCC, viral defense, target cell cytotoxicity assays, cloning and culturing, tumor immunotherapy, and generation of HLA class I transfected target cells. Natural Killer Cell Protocols: Cellular and Molecular Methods offers immunologists, cancer researchers, virologists, and cell biologists today's most comprehensive collection of both established and cutting-edge techniques, methods that will contribute significantly to advancing our understanding of this fascinating and critically important class of cells Biomedicine Immunology Medicine Methode (DE-588)4038971-6 gnd rswk-swf Killerzelle (DE-588)4163776-8 gnd rswk-swf Natürliche Killerzelle (DE-588)4171290-0 gnd rswk-swf 1\p (DE-588)4143413-4 Aufsatzsammlung gnd-content Killerzelle (DE-588)4163776-8 s Methode (DE-588)4038971-6 s DE-604 Natürliche Killerzelle (DE-588)4171290-0 s Campbell, Kerry S. edt Colonna, Marco edt Erscheint auch als Druck-Ausgabe 9780896036833 https://doi.org/10.1385/1592590446 Verlag URL des Erstveröffentlichers Volltext 1\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk |
| spellingShingle | Natural Killer Cell Protocols Cellular and Molecular Methods Biomedicine Immunology Medicine Methode (DE-588)4038971-6 gnd Killerzelle (DE-588)4163776-8 gnd Natürliche Killerzelle (DE-588)4171290-0 gnd |
| subject_GND | (DE-588)4038971-6 (DE-588)4163776-8 (DE-588)4171290-0 (DE-588)4143413-4 |
| title | Natural Killer Cell Protocols Cellular and Molecular Methods |
| title_auth | Natural Killer Cell Protocols Cellular and Molecular Methods |
| title_exact_search | Natural Killer Cell Protocols Cellular and Molecular Methods |
| title_full | Natural Killer Cell Protocols Cellular and Molecular Methods edited by Kerry S. Campbell, Marco Colonna |
| title_fullStr | Natural Killer Cell Protocols Cellular and Molecular Methods edited by Kerry S. Campbell, Marco Colonna |
| title_full_unstemmed | Natural Killer Cell Protocols Cellular and Molecular Methods edited by Kerry S. Campbell, Marco Colonna |
| title_short | Natural Killer Cell Protocols |
| title_sort | natural killer cell protocols cellular and molecular methods |
| title_sub | Cellular and Molecular Methods |
| topic | Biomedicine Immunology Medicine Methode (DE-588)4038971-6 gnd Killerzelle (DE-588)4163776-8 gnd Natürliche Killerzelle (DE-588)4171290-0 gnd |
| topic_facet | Biomedicine Immunology Medicine Methode Killerzelle Natürliche Killerzelle Aufsatzsammlung |
| url | https://doi.org/10.1385/1592590446 |
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