Gene Knockout Protocols:
As the major task of sequencing the human genome nears completion and the full complement of human genes are catalogued, the task of understanding the normal biological functions of genes and how their alteration leads to diseased states becomes more imperative. In Gene Knockout Protocols, highly sk...
Gespeichert in:
| Weitere Verfasser: | , |
|---|---|
| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
Totowa, NJ
Humana Press
2001
|
| Schriftenreihe: | Methods in Molecular Biology
158 |
| Schlagworte: | |
| Online-Zugang: | UBR01 TUM01 URL des Erstveröffentlichers |
| Zusammenfassung: | As the major task of sequencing the human genome nears completion and the full complement of human genes are catalogued, the task of understanding the normal biological functions of genes and how their alteration leads to diseased states becomes more imperative. In Gene Knockout Protocols, highly skilled investigators with extensive experience in gene targeting and mouse genetics describe their best techniques for the design of targeting constructs and for genetic phenotype analysis. These proven methods contain step-by-step instructions, as well as notes on pitfalls to avoid, and emphasize techniques that are relevant to researchers carrying out gene targeting work. These include embryo transplantation, in vitro embryonic stem cell differentiation, creation of aggregation chimeras, mouse pathology, embryo cryopreservation, and transplantation. Issues such as the use of existing mouse mutation resources and the influence of genetic background and epigenetic effects upon phenotype are also covered. State-of-the-art and highly practical, Gene Knockout Protocols not only constitutes an invaluable source of readily reproducible techniques for those just entering the field of gene targeting, but also a key reference for all genetic researchers today |
| Beschreibung: | 1 Online-Ressource (XI, 431 p) |
| ISBN: | 9781592592203 |
| DOI: | 10.1385/1592592201 |
Internformat
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| 520 | |a As the major task of sequencing the human genome nears completion and the full complement of human genes are catalogued, the task of understanding the normal biological functions of genes and how their alteration leads to diseased states becomes more imperative. In Gene Knockout Protocols, highly skilled investigators with extensive experience in gene targeting and mouse genetics describe their best techniques for the design of targeting constructs and for genetic phenotype analysis. These proven methods contain step-by-step instructions, as well as notes on pitfalls to avoid, and emphasize techniques that are relevant to researchers carrying out gene targeting work. These include embryo transplantation, in vitro embryonic stem cell differentiation, creation of aggregation chimeras, mouse pathology, embryo cryopreservation, and transplantation. Issues such as the use of existing mouse mutation resources and the influence of genetic background and epigenetic effects upon phenotype are also covered. State-of-the-art and highly practical, Gene Knockout Protocols not only constitutes an invaluable source of readily reproducible techniques for those just entering the field of gene targeting, but also a key reference for all genetic researchers today | ||
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Datensatz im Suchindex
| _version_ | 1804178538709909504 |
|---|---|
| any_adam_object | |
| author2 | Tymms, Martin J. Kola, Ismail |
| author2_role | edt edt |
| author2_variant | m j t mj mjt i k ik |
| author_facet | Tymms, Martin J. Kola, Ismail |
| building | Verbundindex |
| bvnumber | BV044950815 |
| callnumber-first | Q - Science |
| callnumber-label | QH442 |
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| callnumber-search | QH442.3 |
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| callnumber-subject | QH - Natural History and Biology |
| classification_rvk | WC 4150 WG 3450 |
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| dewey-full | 611.01816 599.935 572.8/77 |
| dewey-hundreds | 600 - Technology (Applied sciences) 500 - Natural sciences and mathematics |
| dewey-ones | 611 - Human anatomy, cytology, histology 599 - Mammalia 572 - Biochemistry |
| dewey-raw | 611.01816 599.935 572.8/77 |
| dewey-search | 611.01816 599.935 572.8/77 |
| dewey-sort | 3611.01816 |
| dewey-tens | 610 - Medicine and health 590 - Animals 570 - Biology |
| discipline | Biologie Chemie-Ingenieurwesen Biotechnologie Medizin |
| doi_str_mv | 10.1385/1592592201 |
| format | Electronic eBook |
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| spelling | Gene Knockout Protocols edited by Martin J. Tymms, Ismail Kola Totowa, NJ Humana Press 2001 1 Online-Ressource (XI, 431 p) txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology 158 As the major task of sequencing the human genome nears completion and the full complement of human genes are catalogued, the task of understanding the normal biological functions of genes and how their alteration leads to diseased states becomes more imperative. In Gene Knockout Protocols, highly skilled investigators with extensive experience in gene targeting and mouse genetics describe their best techniques for the design of targeting constructs and for genetic phenotype analysis. These proven methods contain step-by-step instructions, as well as notes on pitfalls to avoid, and emphasize techniques that are relevant to researchers carrying out gene targeting work. These include embryo transplantation, in vitro embryonic stem cell differentiation, creation of aggregation chimeras, mouse pathology, embryo cryopreservation, and transplantation. Issues such as the use of existing mouse mutation resources and the influence of genetic background and epigenetic effects upon phenotype are also covered. State-of-the-art and highly practical, Gene Knockout Protocols not only constitutes an invaluable source of readily reproducible techniques for those just entering the field of gene targeting, but also a key reference for all genetic researchers today Biomedicine Human Genetics Medicine Human genetics Gen (DE-588)4128987-0 gnd rswk-swf Gen-Targeting (DE-588)4294998-1 gnd rswk-swf Transgene Tiere (DE-588)4279021-9 gnd rswk-swf Maus (DE-588)4169148-9 gnd rswk-swf Mutante (DE-588)4256709-9 gnd rswk-swf 1\p (DE-588)4143413-4 Aufsatzsammlung gnd-content Maus (DE-588)4169148-9 s Mutante (DE-588)4256709-9 s Gen (DE-588)4128987-0 s DE-604 Transgene Tiere (DE-588)4279021-9 s Gen-Targeting (DE-588)4294998-1 s Tymms, Martin J. edt Kola, Ismail edt Erscheint auch als Druck-Ausgabe 9780896035720 https://doi.org/10.1385/1592592201 Verlag URL des Erstveröffentlichers Volltext 1\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk |
| spellingShingle | Gene Knockout Protocols Biomedicine Human Genetics Medicine Human genetics Gen (DE-588)4128987-0 gnd Gen-Targeting (DE-588)4294998-1 gnd Transgene Tiere (DE-588)4279021-9 gnd Maus (DE-588)4169148-9 gnd Mutante (DE-588)4256709-9 gnd |
| subject_GND | (DE-588)4128987-0 (DE-588)4294998-1 (DE-588)4279021-9 (DE-588)4169148-9 (DE-588)4256709-9 (DE-588)4143413-4 |
| title | Gene Knockout Protocols |
| title_auth | Gene Knockout Protocols |
| title_exact_search | Gene Knockout Protocols |
| title_full | Gene Knockout Protocols edited by Martin J. Tymms, Ismail Kola |
| title_fullStr | Gene Knockout Protocols edited by Martin J. Tymms, Ismail Kola |
| title_full_unstemmed | Gene Knockout Protocols edited by Martin J. Tymms, Ismail Kola |
| title_short | Gene Knockout Protocols |
| title_sort | gene knockout protocols |
| topic | Biomedicine Human Genetics Medicine Human genetics Gen (DE-588)4128987-0 gnd Gen-Targeting (DE-588)4294998-1 gnd Transgene Tiere (DE-588)4279021-9 gnd Maus (DE-588)4169148-9 gnd Mutante (DE-588)4256709-9 gnd |
| topic_facet | Biomedicine Human Genetics Medicine Human genetics Gen Gen-Targeting Transgene Tiere Maus Mutante Aufsatzsammlung |
| url | https://doi.org/10.1385/1592592201 |
| work_keys_str_mv | AT tymmsmartinj geneknockoutprotocols AT kolaismail geneknockoutprotocols |