Whole Genome Amplification: Methods and Protocols
This volume mirrors the holistic feature of whole genome amplification (WGA) technology by combining reviews, detailed basic methods, and advanced sample workflows. The first part of the book covers an overview of the development of WGA techniques throughout recent years including general considerat...
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| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
New York, NY
Springer New York
2015
|
| Ausgabe: | 1st ed. 2015 |
| Schriftenreihe: | Methods in Molecular Biology
1347 |
| Schlagworte: | |
| Online-Zugang: | UBR01 TUM01 URL des Erstveröffentlichers |
| Zusammenfassung: | This volume mirrors the holistic feature of whole genome amplification (WGA) technology by combining reviews, detailed basic methods, and advanced sample workflows. The first part of the book covers an overview of the development of WGA techniques throughout recent years including general considerations on bias in WGA, possible sample pre-enrichment strategies and how to run a single-cell lab. The second part focuses on major WGA methods and protocols that allow the assessment of WGA product quality. The final chapters contain advanced protocols and address issues such as sample preparation using laser-microdissection; WGA from partially degraded DNA (formalin-fixed paraffin embedded samples); circulating tumor cells; and ancient samples. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and thorough, Whole Genome Amplification: Methods and Protocols will serve as a rich source of detailed information and inspiration, and will help researchers, both new and experienced, yield successful results. |
| Beschreibung: | 1 Online-Ressource (X, 284 p. 18 illus., 13 illus. in color) |
| ISBN: | 9781493929900 |
| DOI: | 10.1007/978-1-4939-2990-0 |
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| dewey-hundreds | 600 - Technology (Applied sciences) 500 - Natural sciences and mathematics |
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| discipline | Biologie Medizin |
| doi_str_mv | 10.1007/978-1-4939-2990-0 |
| edition | 1st ed. 2015 |
| format | Electronic eBook |
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| id | DE-604.BV044950786 |
| illustrated | Not Illustrated |
| indexdate | 2024-07-10T08:05:34Z |
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| isbn | 9781493929900 |
| language | English |
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| spelling | Whole Genome Amplification Methods and Protocols edited by Thomas Kroneis 1st ed. 2015 New York, NY Springer New York 2015 1 Online-Ressource (X, 284 p. 18 illus., 13 illus. in color) txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology 1347 This volume mirrors the holistic feature of whole genome amplification (WGA) technology by combining reviews, detailed basic methods, and advanced sample workflows. The first part of the book covers an overview of the development of WGA techniques throughout recent years including general considerations on bias in WGA, possible sample pre-enrichment strategies and how to run a single-cell lab. The second part focuses on major WGA methods and protocols that allow the assessment of WGA product quality. The final chapters contain advanced protocols and address issues such as sample preparation using laser-microdissection; WGA from partially degraded DNA (formalin-fixed paraffin embedded samples); circulating tumor cells; and ancient samples. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and thorough, Whole Genome Amplification: Methods and Protocols will serve as a rich source of detailed information and inspiration, and will help researchers, both new and experienced, yield successful results. Biomedicine Human Genetics Medicine Human genetics Kroneis, Thomas edt Erscheint auch als Druck-Ausgabe 9781493929894 https://doi.org/10.1007/978-1-4939-2990-0 Verlag URL des Erstveröffentlichers Volltext |
| spellingShingle | Whole Genome Amplification Methods and Protocols Biomedicine Human Genetics Medicine Human genetics |
| title | Whole Genome Amplification Methods and Protocols |
| title_auth | Whole Genome Amplification Methods and Protocols |
| title_exact_search | Whole Genome Amplification Methods and Protocols |
| title_full | Whole Genome Amplification Methods and Protocols edited by Thomas Kroneis |
| title_fullStr | Whole Genome Amplification Methods and Protocols edited by Thomas Kroneis |
| title_full_unstemmed | Whole Genome Amplification Methods and Protocols edited by Thomas Kroneis |
| title_short | Whole Genome Amplification |
| title_sort | whole genome amplification methods and protocols |
| title_sub | Methods and Protocols |
| topic | Biomedicine Human Genetics Medicine Human genetics |
| topic_facet | Biomedicine Human Genetics Medicine Human genetics |
| url | https://doi.org/10.1007/978-1-4939-2990-0 |
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