Patch-Clamp Applications and Protocols:
E. Neher and B. Sakman were the first to monitor the opening and closing of single ion channels and membranes by conductance measurements. In 1976, they used firepolished micropipets with a tip diameter of 3-5 pm to record currents from a small patch of the membranbe of sk- etal muscles, thereby dec...
Gespeichert in:
| Weitere Verfasser: | , , |
|---|---|
| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
Totowa, NJ
Humana Press
1995
|
| Schriftenreihe: | Neuromethods
26 |
| Schlagworte: | |
| Online-Zugang: | UBR01 Volltext |
| Zusammenfassung: | E. Neher and B. Sakman were the first to monitor the opening and closing of single ion channels and membranes by conductance measurements. In 1976, they used firepolished micropipets with a tip diameter of 3-5 pm to record currents from a small patch of the membranbe of sk- etal muscles, thereby decreasing background membrane noise. In order to reduce the dominant source of background noise-the leakage shunt under the pipet rim between m- the muscle membrane had to be treated brane and gla- enzymatically. Despite these early limitations, a new te- nique was born -the patch-clamp technique. The final bre- through came in 1981 when the same authors, in collaboration with 0. P. Hamill, A. Marty, and F. J. Sigworth, developed the gigaohm seal. Not only did this improve the quality of recordings, it was now possible to gently pull the membrane patch with the attached pipet off the cell and study its trapped ion channels in isolation. Another offshoot of the gigaohm seal technique was the whole-cell patch-clamp technique, in which the patch is ruptured without breaking the seal. This technique is really a sophisticated voltage-clamp technique and also allows for the altering of cytoplasmic constituents if the experimenter so wishes. The first part of Patch-Clamp Applications and Protocols presents modern developments associated with the techn- ogy of patch-clamp electrodes, of cell-free ion channel reco- ing, and of the whole-cell patch-clamp technique |
| Beschreibung: | 1 Online-Ressource (XVI, 316 p) |
| ISBN: | 9781592596317 |
| DOI: | 10.1385/0896033112 |
Internformat
MARC
| LEADER | 00000nmm a2200000zcb4500 | ||
|---|---|---|---|
| 001 | BV044950776 | ||
| 003 | DE-604 | ||
| 005 | 20191030 | ||
| 007 | cr|uuu---uuuuu | ||
| 008 | 180517s1995 |||| o||u| ||||||eng d | ||
| 020 | |a 9781592596317 |9 978-1-59259-631-7 | ||
| 024 | 7 | |a 10.1385/0896033112 |2 doi | |
| 035 | |a (ZDB-2-PRO)978-1-59259-631-7 | ||
| 035 | |a (OCoLC)1036368076 | ||
| 035 | |a (DE-599)BVBBV044950776 | ||
| 040 | |a DE-604 |b ger |e rda | ||
| 041 | 0 | |a eng | |
| 049 | |a DE-355 | ||
| 050 | 0 | |a QH585 | |
| 082 | 0 | |a 571.6 |2 21 | |
| 082 | 0 | |a 612.8 |2 23 | |
| 084 | |a WC 5150 |0 (DE-625)148109:13423 |2 rvk | ||
| 084 | |a WW 2200 |0 (DE-625)158906:13423 |2 rvk | ||
| 084 | |a QU 375 |2 nlm | ||
| 245 | 1 | 0 | |a Patch-Clamp Applications and Protocols |c edited by Alan A. Boulton, Glen B. Baker, Wolfgang Walz |
| 264 | 1 | |a Totowa, NJ |b Humana Press |c 1995 | |
| 300 | |a 1 Online-Ressource (XVI, 316 p) | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b c |2 rdamedia | ||
| 338 | |b cr |2 rdacarrier | ||
| 490 | 0 | |a Neuromethods |v 26 | |
| 520 | |a E. Neher and B. Sakman were the first to monitor the opening and closing of single ion channels and membranes by conductance measurements. In 1976, they used firepolished micropipets with a tip diameter of 3-5 pm to record currents from a small patch of the membranbe of sk- etal muscles, thereby decreasing background membrane noise. In order to reduce the dominant source of background noise-the leakage shunt under the pipet rim between m- the muscle membrane had to be treated brane and gla- enzymatically. Despite these early limitations, a new te- nique was born -the patch-clamp technique. The final bre- through came in 1981 when the same authors, in collaboration with 0. P. Hamill, A. Marty, and F. J. Sigworth, developed the gigaohm seal. Not only did this improve the quality of recordings, it was now possible to gently pull the membrane patch with the attached pipet off the cell and study its trapped ion channels in isolation. Another offshoot of the gigaohm seal technique was the whole-cell patch-clamp technique, in which the patch is ruptured without breaking the seal. This technique is really a sophisticated voltage-clamp technique and also allows for the altering of cytoplasmic constituents if the experimenter so wishes. The first part of Patch-Clamp Applications and Protocols presents modern developments associated with the techn- ogy of patch-clamp electrodes, of cell-free ion channel reco- ing, and of the whole-cell patch-clamp technique | ||
| 650 | 4 | |a Biomedicine | |
| 650 | 4 | |a Neurosciences | |
| 650 | 4 | |a Medicine | |
| 650 | 4 | |a Neurosciences | |
| 650 | 0 | 7 | |a Patch-Clamp-Methode |0 (DE-588)4279564-3 |2 gnd |9 rswk-swf |
| 689 | 0 | 0 | |a Patch-Clamp-Methode |0 (DE-588)4279564-3 |D s |
| 689 | 0 | |5 DE-604 | |
| 700 | 1 | |a Boulton, Alan A. |4 edt | |
| 700 | 1 | |a Baker, Glen B. |4 edt | |
| 700 | 1 | |a Walz, Wolfgang |4 edt | |
| 776 | 0 | 8 | |i Erscheint auch als |n Druck-Ausgabe |z 9780896033115 |
| 856 | 4 | 0 | |u https://doi.org/10.1385/0896033112 |x Verlag |z URL des Erstveröffentlichers |3 Volltext |
| 912 | |a ZDB-2-PRO | ||
| 999 | |a oai:aleph.bib-bvb.de:BVB01-030343535 | ||
| 966 | e | |u https://doi.org/10.1385/0896033112 |l UBR01 |p ZDB-2-PRO |x Verlag |3 Volltext | |
Datensatz im Suchindex
| _version_ | 1804178538607149056 |
|---|---|
| any_adam_object | |
| author2 | Boulton, Alan A. Baker, Glen B. Walz, Wolfgang |
| author2_role | edt edt edt |
| author2_variant | a a b aa aab g b b gb gbb w w ww |
| author_facet | Boulton, Alan A. Baker, Glen B. Walz, Wolfgang |
| building | Verbundindex |
| bvnumber | BV044950776 |
| callnumber-first | Q - Science |
| callnumber-label | QH585 |
| callnumber-raw | QH585 |
| callnumber-search | QH585 |
| callnumber-sort | QH 3585 |
| callnumber-subject | QH - Natural History and Biology |
| classification_rvk | WC 5150 WW 2200 |
| collection | ZDB-2-PRO |
| ctrlnum | (ZDB-2-PRO)978-1-59259-631-7 (OCoLC)1036368076 (DE-599)BVBBV044950776 |
| dewey-full | 571.6 612.8 |
| dewey-hundreds | 500 - Natural sciences and mathematics 600 - Technology (Applied sciences) |
| dewey-ones | 571 - Physiology & related subjects 612 - Human physiology |
| dewey-raw | 571.6 612.8 |
| dewey-search | 571.6 612.8 |
| dewey-sort | 3571.6 |
| dewey-tens | 570 - Biology 610 - Medicine and health |
| discipline | Biologie Medizin |
| doi_str_mv | 10.1385/0896033112 |
| format | Electronic eBook |
| fullrecord | <?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>03188nmm a2200529zcb4500</leader><controlfield tag="001">BV044950776</controlfield><controlfield tag="003">DE-604</controlfield><controlfield tag="005">20191030 </controlfield><controlfield tag="007">cr|uuu---uuuuu</controlfield><controlfield tag="008">180517s1995 |||| o||u| ||||||eng d</controlfield><datafield tag="020" ind1=" " ind2=" "><subfield code="a">9781592596317</subfield><subfield code="9">978-1-59259-631-7</subfield></datafield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1385/0896033112</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(ZDB-2-PRO)978-1-59259-631-7</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(OCoLC)1036368076</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)BVBBV044950776</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-604</subfield><subfield code="b">ger</subfield><subfield code="e">rda</subfield></datafield><datafield tag="041" ind1="0" ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="049" ind1=" " ind2=" "><subfield code="a">DE-355</subfield></datafield><datafield tag="050" ind1=" " ind2="0"><subfield code="a">QH585</subfield></datafield><datafield tag="082" ind1="0" ind2=" "><subfield code="a">571.6</subfield><subfield code="2">21</subfield></datafield><datafield tag="082" ind1="0" ind2=" "><subfield code="a">612.8</subfield><subfield code="2">23</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">WC 5150</subfield><subfield code="0">(DE-625)148109:13423</subfield><subfield code="2">rvk</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">WW 2200</subfield><subfield code="0">(DE-625)158906:13423</subfield><subfield code="2">rvk</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">QU 375</subfield><subfield code="2">nlm</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Patch-Clamp Applications and Protocols</subfield><subfield code="c">edited by Alan A. Boulton, Glen B. Baker, Wolfgang Walz</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="a">Totowa, NJ</subfield><subfield code="b">Humana Press</subfield><subfield code="c">1995</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">1 Online-Ressource (XVI, 316 p)</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="490" ind1="0" ind2=" "><subfield code="a">Neuromethods</subfield><subfield code="v">26</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">E. Neher and B. Sakman were the first to monitor the opening and closing of single ion channels and membranes by conductance measurements. In 1976, they used firepolished micropipets with a tip diameter of 3-5 pm to record currents from a small patch of the membranbe of sk- etal muscles, thereby decreasing background membrane noise. In order to reduce the dominant source of background noise-the leakage shunt under the pipet rim between m- the muscle membrane had to be treated brane and gla- enzymatically. Despite these early limitations, a new te- nique was born -the patch-clamp technique. The final bre- through came in 1981 when the same authors, in collaboration with 0. P. Hamill, A. Marty, and F. J. Sigworth, developed the gigaohm seal. Not only did this improve the quality of recordings, it was now possible to gently pull the membrane patch with the attached pipet off the cell and study its trapped ion channels in isolation. Another offshoot of the gigaohm seal technique was the whole-cell patch-clamp technique, in which the patch is ruptured without breaking the seal. This technique is really a sophisticated voltage-clamp technique and also allows for the altering of cytoplasmic constituents if the experimenter so wishes. The first part of Patch-Clamp Applications and Protocols presents modern developments associated with the techn- ogy of patch-clamp electrodes, of cell-free ion channel reco- ing, and of the whole-cell patch-clamp technique</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Biomedicine</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Neurosciences</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Medicine</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Neurosciences</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Patch-Clamp-Methode</subfield><subfield code="0">(DE-588)4279564-3</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="689" ind1="0" ind2="0"><subfield code="a">Patch-Clamp-Methode</subfield><subfield code="0">(DE-588)4279564-3</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2=" "><subfield code="5">DE-604</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Boulton, Alan A.</subfield><subfield code="4">edt</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Baker, Glen B.</subfield><subfield code="4">edt</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Walz, Wolfgang</subfield><subfield code="4">edt</subfield></datafield><datafield tag="776" ind1="0" ind2="8"><subfield code="i">Erscheint auch als</subfield><subfield code="n">Druck-Ausgabe</subfield><subfield code="z">9780896033115</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://doi.org/10.1385/0896033112</subfield><subfield code="x">Verlag</subfield><subfield code="z">URL des Erstveröffentlichers</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">ZDB-2-PRO</subfield></datafield><datafield tag="999" ind1=" " ind2=" "><subfield code="a">oai:aleph.bib-bvb.de:BVB01-030343535</subfield></datafield><datafield tag="966" ind1="e" ind2=" "><subfield code="u">https://doi.org/10.1385/0896033112</subfield><subfield code="l">UBR01</subfield><subfield code="p">ZDB-2-PRO</subfield><subfield code="x">Verlag</subfield><subfield code="3">Volltext</subfield></datafield></record></collection> |
| id | DE-604.BV044950776 |
| illustrated | Not Illustrated |
| indexdate | 2024-07-10T08:05:34Z |
| institution | BVB |
| isbn | 9781592596317 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-030343535 |
| oclc_num | 1036368076 |
| open_access_boolean | |
| owner | DE-355 DE-BY-UBR |
| owner_facet | DE-355 DE-BY-UBR |
| physical | 1 Online-Ressource (XVI, 316 p) |
| psigel | ZDB-2-PRO |
| publishDate | 1995 |
| publishDateSearch | 1995 |
| publishDateSort | 1995 |
| publisher | Humana Press |
| record_format | marc |
| series2 | Neuromethods |
| spelling | Patch-Clamp Applications and Protocols edited by Alan A. Boulton, Glen B. Baker, Wolfgang Walz Totowa, NJ Humana Press 1995 1 Online-Ressource (XVI, 316 p) txt rdacontent c rdamedia cr rdacarrier Neuromethods 26 E. Neher and B. Sakman were the first to monitor the opening and closing of single ion channels and membranes by conductance measurements. In 1976, they used firepolished micropipets with a tip diameter of 3-5 pm to record currents from a small patch of the membranbe of sk- etal muscles, thereby decreasing background membrane noise. In order to reduce the dominant source of background noise-the leakage shunt under the pipet rim between m- the muscle membrane had to be treated brane and gla- enzymatically. Despite these early limitations, a new te- nique was born -the patch-clamp technique. The final bre- through came in 1981 when the same authors, in collaboration with 0. P. Hamill, A. Marty, and F. J. Sigworth, developed the gigaohm seal. Not only did this improve the quality of recordings, it was now possible to gently pull the membrane patch with the attached pipet off the cell and study its trapped ion channels in isolation. Another offshoot of the gigaohm seal technique was the whole-cell patch-clamp technique, in which the patch is ruptured without breaking the seal. This technique is really a sophisticated voltage-clamp technique and also allows for the altering of cytoplasmic constituents if the experimenter so wishes. The first part of Patch-Clamp Applications and Protocols presents modern developments associated with the techn- ogy of patch-clamp electrodes, of cell-free ion channel reco- ing, and of the whole-cell patch-clamp technique Biomedicine Neurosciences Medicine Patch-Clamp-Methode (DE-588)4279564-3 gnd rswk-swf Patch-Clamp-Methode (DE-588)4279564-3 s DE-604 Boulton, Alan A. edt Baker, Glen B. edt Walz, Wolfgang edt Erscheint auch als Druck-Ausgabe 9780896033115 https://doi.org/10.1385/0896033112 Verlag URL des Erstveröffentlichers Volltext |
| spellingShingle | Patch-Clamp Applications and Protocols Biomedicine Neurosciences Medicine Patch-Clamp-Methode (DE-588)4279564-3 gnd |
| subject_GND | (DE-588)4279564-3 |
| title | Patch-Clamp Applications and Protocols |
| title_auth | Patch-Clamp Applications and Protocols |
| title_exact_search | Patch-Clamp Applications and Protocols |
| title_full | Patch-Clamp Applications and Protocols edited by Alan A. Boulton, Glen B. Baker, Wolfgang Walz |
| title_fullStr | Patch-Clamp Applications and Protocols edited by Alan A. Boulton, Glen B. Baker, Wolfgang Walz |
| title_full_unstemmed | Patch-Clamp Applications and Protocols edited by Alan A. Boulton, Glen B. Baker, Wolfgang Walz |
| title_short | Patch-Clamp Applications and Protocols |
| title_sort | patch clamp applications and protocols |
| topic | Biomedicine Neurosciences Medicine Patch-Clamp-Methode (DE-588)4279564-3 gnd |
| topic_facet | Biomedicine Neurosciences Medicine Patch-Clamp-Methode |
| url | https://doi.org/10.1385/0896033112 |
| work_keys_str_mv | AT boultonalana patchclampapplicationsandprotocols AT bakerglenb patchclampapplicationsandprotocols AT walzwolfgang patchclampapplicationsandprotocols |