Quantitative Real-Time PCR: Methods and Protocols
Quantitative Real-Time PCR: Methods and Protocols focuses on different applications of qPCR ranging from microbiological detections (both viral and bacterial) to pathological applications. Several chapters deal with quality issues which regard the quality of starting material, the knowledge of the m...
Gespeichert in:
| Weitere Verfasser: | , |
|---|---|
| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
New York, NY
Springer New York
2014
|
| Schriftenreihe: | Methods in Molecular Biology, Methods and Protocols
1160 |
| Schlagworte: | |
| Online-Zugang: | UBR01 TUM01 Volltext |
| Zusammenfassung: | Quantitative Real-Time PCR: Methods and Protocols focuses on different applications of qPCR ranging from microbiological detections (both viral and bacterial) to pathological applications. Several chapters deal with quality issues which regard the quality of starting material, the knowledge of the minimal information required to both perform an assay and to set the experimental plan, while the others focus on translational medicine applications that are ordered following an approximate logical order of their medical application. The last part of the book gives you an idea of an emerging digital PCR technique that is a unique qPCR approach for measuring nucleic acid, particularly suited for low level detection and to develop non-invasive diagnosis. Written for the Methods in Molecular Biology series, most chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and authoritative, Quantitative Real-Time PCR: Methods and Protocols aims to aid researchers seeking to devise new qPCR-based approaches related to his or her area of investigation |
| Beschreibung: | 1 Online-Ressource (XI, 231 p. 80 illus., 66 illus. in color) |
| ISBN: | 9781493907335 |
| DOI: | 10.1007/978-1-4939-0733-5 |
Internformat
MARC
| LEADER | 00000nmm a2200000zcb4500 | ||
|---|---|---|---|
| 001 | BV044950767 | ||
| 003 | DE-604 | ||
| 005 | 00000000000000.0 | ||
| 007 | cr|uuu---uuuuu | ||
| 008 | 180517s2014 |||| o||u| ||||||eng d | ||
| 020 | |a 9781493907335 |9 978-1-4939-0733-5 | ||
| 024 | 7 | |a 10.1007/978-1-4939-0733-5 |2 doi | |
| 035 | |a (ZDB-2-PRO)978-1-4939-0733-5 | ||
| 035 | |a (OCoLC)1036383558 | ||
| 035 | |a (DE-599)BVBBV044950767 | ||
| 040 | |a DE-604 |b ger |e rda | ||
| 041 | 0 | |a eng | |
| 049 | |a DE-355 |a DE-91 | ||
| 082 | 0 | |a 591.35 |2 23 | |
| 084 | |a WC 4460 |0 (DE-625)148102: |2 rvk | ||
| 245 | 1 | 0 | |a Quantitative Real-Time PCR |b Methods and Protocols |c edited by Roberto Biassoni, Alessandro Raso |
| 264 | 1 | |a New York, NY |b Springer New York |c 2014 | |
| 300 | |a 1 Online-Ressource (XI, 231 p. 80 illus., 66 illus. in color) | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b c |2 rdamedia | ||
| 338 | |b cr |2 rdacarrier | ||
| 490 | 0 | |a Methods in Molecular Biology, Methods and Protocols |v 1160 | |
| 520 | |a Quantitative Real-Time PCR: Methods and Protocols focuses on different applications of qPCR ranging from microbiological detections (both viral and bacterial) to pathological applications. Several chapters deal with quality issues which regard the quality of starting material, the knowledge of the minimal information required to both perform an assay and to set the experimental plan, while the others focus on translational medicine applications that are ordered following an approximate logical order of their medical application. The last part of the book gives you an idea of an emerging digital PCR technique that is a unique qPCR approach for measuring nucleic acid, particularly suited for low level detection and to develop non-invasive diagnosis. Written for the Methods in Molecular Biology series, most chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and authoritative, Quantitative Real-Time PCR: Methods and Protocols aims to aid researchers seeking to devise new qPCR-based approaches related to his or her area of investigation | ||
| 650 | 4 | |a Life Sciences | |
| 650 | 4 | |a Animal Genetics and Genomics | |
| 650 | 4 | |a Human Genetics | |
| 650 | 4 | |a Life sciences | |
| 650 | 4 | |a Human genetics | |
| 650 | 4 | |a Animal genetics | |
| 650 | 0 | 7 | |a Polymerase-Kettenreaktion |0 (DE-588)4256726-9 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Quantitative Analyse |0 (DE-588)4125311-5 |2 gnd |9 rswk-swf |
| 689 | 0 | 0 | |a Polymerase-Kettenreaktion |0 (DE-588)4256726-9 |D s |
| 689 | 0 | 1 | |a Quantitative Analyse |0 (DE-588)4125311-5 |D s |
| 689 | 0 | |8 1\p |5 DE-604 | |
| 700 | 1 | |a Biassoni, Roberto |4 edt | |
| 700 | 1 | |a Raso, Alessandro |4 edt | |
| 776 | 0 | 8 | |i Erscheint auch als |n Druck-Ausgabe |z 9781493907328 |
| 856 | 4 | 0 | |u https://doi.org/10.1007/978-1-4939-0733-5 |x Verlag |z URL des Erstveröffentlichers |3 Volltext |
| 912 | |a ZDB-2-PRO | ||
| 999 | |a oai:aleph.bib-bvb.de:BVB01-030343526 | ||
| 883 | 1 | |8 1\p |a cgwrk |d 20201028 |q DE-101 |u https://d-nb.info/provenance/plan#cgwrk | |
| 966 | e | |u https://doi.org/10.1007/978-1-4939-0733-5 |l UBR01 |p ZDB-2-PRO |x Verlag |3 Volltext | |
| 966 | e | |u https://doi.org/10.1007/978-1-4939-0733-5 |l TUM01 |p ZDB-2-PRO |x Verlag |3 Volltext | |
Datensatz im Suchindex
| _version_ | 1804178538592468992 |
|---|---|
| any_adam_object | |
| author2 | Biassoni, Roberto Raso, Alessandro |
| author2_role | edt edt |
| author2_variant | r b rb a r ar |
| author_facet | Biassoni, Roberto Raso, Alessandro |
| building | Verbundindex |
| bvnumber | BV044950767 |
| classification_rvk | WC 4460 |
| collection | ZDB-2-PRO |
| ctrlnum | (ZDB-2-PRO)978-1-4939-0733-5 (OCoLC)1036383558 (DE-599)BVBBV044950767 |
| dewey-full | 591.35 |
| dewey-hundreds | 500 - Natural sciences and mathematics |
| dewey-ones | 591 - Specific topics in natural history of animals |
| dewey-raw | 591.35 |
| dewey-search | 591.35 |
| dewey-sort | 3591.35 |
| dewey-tens | 590 - Animals |
| discipline | Biologie |
| doi_str_mv | 10.1007/978-1-4939-0733-5 |
| format | Electronic eBook |
| fullrecord | <?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>03280nmm a2200541zcb4500</leader><controlfield tag="001">BV044950767</controlfield><controlfield tag="003">DE-604</controlfield><controlfield tag="005">00000000000000.0</controlfield><controlfield tag="007">cr|uuu---uuuuu</controlfield><controlfield tag="008">180517s2014 |||| o||u| ||||||eng d</controlfield><datafield tag="020" ind1=" " ind2=" "><subfield code="a">9781493907335</subfield><subfield code="9">978-1-4939-0733-5</subfield></datafield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1007/978-1-4939-0733-5</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(ZDB-2-PRO)978-1-4939-0733-5</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(OCoLC)1036383558</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)BVBBV044950767</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-604</subfield><subfield code="b">ger</subfield><subfield code="e">rda</subfield></datafield><datafield tag="041" ind1="0" ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="049" ind1=" " ind2=" "><subfield code="a">DE-355</subfield><subfield code="a">DE-91</subfield></datafield><datafield tag="082" ind1="0" ind2=" "><subfield code="a">591.35</subfield><subfield code="2">23</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">WC 4460</subfield><subfield code="0">(DE-625)148102:</subfield><subfield code="2">rvk</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Quantitative Real-Time PCR</subfield><subfield code="b">Methods and Protocols</subfield><subfield code="c">edited by Roberto Biassoni, Alessandro Raso</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="a">New York, NY</subfield><subfield code="b">Springer New York</subfield><subfield code="c">2014</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">1 Online-Ressource (XI, 231 p. 80 illus., 66 illus. in color)</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="490" ind1="0" ind2=" "><subfield code="a">Methods in Molecular Biology, Methods and Protocols</subfield><subfield code="v">1160</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Quantitative Real-Time PCR: Methods and Protocols focuses on different applications of qPCR ranging from microbiological detections (both viral and bacterial) to pathological applications. Several chapters deal with quality issues which regard the quality of starting material, the knowledge of the minimal information required to both perform an assay and to set the experimental plan, while the others focus on translational medicine applications that are ordered following an approximate logical order of their medical application. The last part of the book gives you an idea of an emerging digital PCR technique that is a unique qPCR approach for measuring nucleic acid, particularly suited for low level detection and to develop non-invasive diagnosis. Written for the Methods in Molecular Biology series, most chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and authoritative, Quantitative Real-Time PCR: Methods and Protocols aims to aid researchers seeking to devise new qPCR-based approaches related to his or her area of investigation</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Life Sciences</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Animal Genetics and Genomics</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Human Genetics</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Life sciences</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Human genetics</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Animal genetics</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Polymerase-Kettenreaktion</subfield><subfield code="0">(DE-588)4256726-9</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Quantitative Analyse</subfield><subfield code="0">(DE-588)4125311-5</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="689" ind1="0" ind2="0"><subfield code="a">Polymerase-Kettenreaktion</subfield><subfield code="0">(DE-588)4256726-9</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2="1"><subfield code="a">Quantitative Analyse</subfield><subfield code="0">(DE-588)4125311-5</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2=" "><subfield code="8">1\p</subfield><subfield code="5">DE-604</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Biassoni, Roberto</subfield><subfield code="4">edt</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Raso, Alessandro</subfield><subfield code="4">edt</subfield></datafield><datafield tag="776" ind1="0" ind2="8"><subfield code="i">Erscheint auch als</subfield><subfield code="n">Druck-Ausgabe</subfield><subfield code="z">9781493907328</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://doi.org/10.1007/978-1-4939-0733-5</subfield><subfield code="x">Verlag</subfield><subfield code="z">URL des Erstveröffentlichers</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">ZDB-2-PRO</subfield></datafield><datafield tag="999" ind1=" " ind2=" "><subfield code="a">oai:aleph.bib-bvb.de:BVB01-030343526</subfield></datafield><datafield tag="883" ind1="1" ind2=" "><subfield code="8">1\p</subfield><subfield code="a">cgwrk</subfield><subfield code="d">20201028</subfield><subfield code="q">DE-101</subfield><subfield code="u">https://d-nb.info/provenance/plan#cgwrk</subfield></datafield><datafield tag="966" ind1="e" ind2=" "><subfield code="u">https://doi.org/10.1007/978-1-4939-0733-5</subfield><subfield code="l">UBR01</subfield><subfield code="p">ZDB-2-PRO</subfield><subfield code="x">Verlag</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="966" ind1="e" ind2=" "><subfield code="u">https://doi.org/10.1007/978-1-4939-0733-5</subfield><subfield code="l">TUM01</subfield><subfield code="p">ZDB-2-PRO</subfield><subfield code="x">Verlag</subfield><subfield code="3">Volltext</subfield></datafield></record></collection> |
| id | DE-604.BV044950767 |
| illustrated | Not Illustrated |
| indexdate | 2024-07-10T08:05:34Z |
| institution | BVB |
| isbn | 9781493907335 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-030343526 |
| oclc_num | 1036383558 |
| open_access_boolean | |
| owner | DE-355 DE-BY-UBR DE-91 DE-BY-TUM |
| owner_facet | DE-355 DE-BY-UBR DE-91 DE-BY-TUM |
| physical | 1 Online-Ressource (XI, 231 p. 80 illus., 66 illus. in color) |
| psigel | ZDB-2-PRO |
| publishDate | 2014 |
| publishDateSearch | 2014 |
| publishDateSort | 2014 |
| publisher | Springer New York |
| record_format | marc |
| series2 | Methods in Molecular Biology, Methods and Protocols |
| spelling | Quantitative Real-Time PCR Methods and Protocols edited by Roberto Biassoni, Alessandro Raso New York, NY Springer New York 2014 1 Online-Ressource (XI, 231 p. 80 illus., 66 illus. in color) txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology, Methods and Protocols 1160 Quantitative Real-Time PCR: Methods and Protocols focuses on different applications of qPCR ranging from microbiological detections (both viral and bacterial) to pathological applications. Several chapters deal with quality issues which regard the quality of starting material, the knowledge of the minimal information required to both perform an assay and to set the experimental plan, while the others focus on translational medicine applications that are ordered following an approximate logical order of their medical application. The last part of the book gives you an idea of an emerging digital PCR technique that is a unique qPCR approach for measuring nucleic acid, particularly suited for low level detection and to develop non-invasive diagnosis. Written for the Methods in Molecular Biology series, most chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and authoritative, Quantitative Real-Time PCR: Methods and Protocols aims to aid researchers seeking to devise new qPCR-based approaches related to his or her area of investigation Life Sciences Animal Genetics and Genomics Human Genetics Life sciences Human genetics Animal genetics Polymerase-Kettenreaktion (DE-588)4256726-9 gnd rswk-swf Quantitative Analyse (DE-588)4125311-5 gnd rswk-swf Polymerase-Kettenreaktion (DE-588)4256726-9 s Quantitative Analyse (DE-588)4125311-5 s 1\p DE-604 Biassoni, Roberto edt Raso, Alessandro edt Erscheint auch als Druck-Ausgabe 9781493907328 https://doi.org/10.1007/978-1-4939-0733-5 Verlag URL des Erstveröffentlichers Volltext 1\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk |
| spellingShingle | Quantitative Real-Time PCR Methods and Protocols Life Sciences Animal Genetics and Genomics Human Genetics Life sciences Human genetics Animal genetics Polymerase-Kettenreaktion (DE-588)4256726-9 gnd Quantitative Analyse (DE-588)4125311-5 gnd |
| subject_GND | (DE-588)4256726-9 (DE-588)4125311-5 |
| title | Quantitative Real-Time PCR Methods and Protocols |
| title_auth | Quantitative Real-Time PCR Methods and Protocols |
| title_exact_search | Quantitative Real-Time PCR Methods and Protocols |
| title_full | Quantitative Real-Time PCR Methods and Protocols edited by Roberto Biassoni, Alessandro Raso |
| title_fullStr | Quantitative Real-Time PCR Methods and Protocols edited by Roberto Biassoni, Alessandro Raso |
| title_full_unstemmed | Quantitative Real-Time PCR Methods and Protocols edited by Roberto Biassoni, Alessandro Raso |
| title_short | Quantitative Real-Time PCR |
| title_sort | quantitative real time pcr methods and protocols |
| title_sub | Methods and Protocols |
| topic | Life Sciences Animal Genetics and Genomics Human Genetics Life sciences Human genetics Animal genetics Polymerase-Kettenreaktion (DE-588)4256726-9 gnd Quantitative Analyse (DE-588)4125311-5 gnd |
| topic_facet | Life Sciences Animal Genetics and Genomics Human Genetics Life sciences Human genetics Animal genetics Polymerase-Kettenreaktion Quantitative Analyse |
| url | https://doi.org/10.1007/978-1-4939-0733-5 |
| work_keys_str_mv | AT biassoniroberto quantitativerealtimepcrmethodsandprotocols AT rasoalessandro quantitativerealtimepcrmethodsandprotocols |