In Vitro Transcription and Translation Protocols:
A highly anticipated update of the previous edition, In Vitro Transcription and Translation Protocols, Second Edition, provides molecular biology laboratories with the most powerful techniques for exploiting in vitro transcription and translation systems.In Section One: Technologies, authors discuss...
Gespeichert in:
| Weitere Verfasser: | |
|---|---|
| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
Totowa, NJ
Humana Press
2007
|
| Ausgabe: | Second Edition |
| Schriftenreihe: | Methods in Molecular Biology™
375 |
| Schlagworte: | |
| Online-Zugang: | UBR01 TUM01 Volltext |
| Zusammenfassung: | A highly anticipated update of the previous edition, In Vitro Transcription and Translation Protocols, Second Edition, provides molecular biology laboratories with the most powerful techniques for exploiting in vitro transcription and translation systems.In Section One: Technologies, authors discuss the use of alternative energy systems for ATP regeneration, the efficient in vitro production of integral membrane proteins, and the high-throughput production of protein libraries using the innovative single-molecule PRC-linked in vitro expression (SIMPLEX) technology. In Section Two: Applications, authors present in vitro translation of amplified protease gene from HIV patients to select proper anti-protease drug therapy, in vitro translation of genes from human patients to diagnose biologically relevant gene mutations, use of SIMPLEX technology to select enantioselective lipases from libraries of randomly produced lipase mutants, and the in vitro transcription and translation to identify proteins in 2D-maps |
| Beschreibung: | 1 Online-Ressource (XII, 276 p. 51 illus., 1 illus. in color) |
| ISBN: | 9781597453882 |
| DOI: | 10.1007/978-1-59745-388-2 |
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| 520 | |a A highly anticipated update of the previous edition, In Vitro Transcription and Translation Protocols, Second Edition, provides molecular biology laboratories with the most powerful techniques for exploiting in vitro transcription and translation systems.In Section One: Technologies, authors discuss the use of alternative energy systems for ATP regeneration, the efficient in vitro production of integral membrane proteins, and the high-throughput production of protein libraries using the innovative single-molecule PRC-linked in vitro expression (SIMPLEX) technology. In Section Two: Applications, authors present in vitro translation of amplified protease gene from HIV patients to select proper anti-protease drug therapy, in vitro translation of genes from human patients to diagnose biologically relevant gene mutations, use of SIMPLEX technology to select enantioselective lipases from libraries of randomly produced lipase mutants, and the in vitro transcription and translation to identify proteins in 2D-maps | ||
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| dewey-ones | 572 - Biochemistry |
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| dewey-tens | 570 - Biology |
| discipline | Biologie |
| doi_str_mv | 10.1007/978-1-59745-388-2 |
| edition | Second Edition |
| format | Electronic eBook |
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| id | DE-604.BV044950766 |
| illustrated | Not Illustrated |
| indexdate | 2024-07-10T08:05:34Z |
| institution | BVB |
| isbn | 9781597453882 |
| language | English |
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| physical | 1 Online-Ressource (XII, 276 p. 51 illus., 1 illus. in color) |
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| spelling | In Vitro Transcription and Translation Protocols edited by Guido Grandi Second Edition Totowa, NJ Humana Press 2007 1 Online-Ressource (XII, 276 p. 51 illus., 1 illus. in color) txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology™ 375 A highly anticipated update of the previous edition, In Vitro Transcription and Translation Protocols, Second Edition, provides molecular biology laboratories with the most powerful techniques for exploiting in vitro transcription and translation systems.In Section One: Technologies, authors discuss the use of alternative energy systems for ATP regeneration, the efficient in vitro production of integral membrane proteins, and the high-throughput production of protein libraries using the innovative single-molecule PRC-linked in vitro expression (SIMPLEX) technology. In Section Two: Applications, authors present in vitro translation of amplified protease gene from HIV patients to select proper anti-protease drug therapy, in vitro translation of genes from human patients to diagnose biologically relevant gene mutations, use of SIMPLEX technology to select enantioselective lipases from libraries of randomly produced lipase mutants, and the in vitro transcription and translation to identify proteins in 2D-maps Life Sciences Biochemistry, general Human Genetics Cell Biology Proteomics Life sciences Human genetics Biochemistry Cell biology Grandi, Guido edt Erscheint auch als Druck-Ausgabe 9781588295583 https://doi.org/10.1007/978-1-59745-388-2 Verlag URL des Erstveröffentlichers Volltext |
| spellingShingle | In Vitro Transcription and Translation Protocols Life Sciences Biochemistry, general Human Genetics Cell Biology Proteomics Life sciences Human genetics Biochemistry Cell biology |
| title | In Vitro Transcription and Translation Protocols |
| title_auth | In Vitro Transcription and Translation Protocols |
| title_exact_search | In Vitro Transcription and Translation Protocols |
| title_full | In Vitro Transcription and Translation Protocols edited by Guido Grandi |
| title_fullStr | In Vitro Transcription and Translation Protocols edited by Guido Grandi |
| title_full_unstemmed | In Vitro Transcription and Translation Protocols edited by Guido Grandi |
| title_short | In Vitro Transcription and Translation Protocols |
| title_sort | in vitro transcription and translation protocols |
| topic | Life Sciences Biochemistry, general Human Genetics Cell Biology Proteomics Life sciences Human genetics Biochemistry Cell biology |
| topic_facet | Life Sciences Biochemistry, general Human Genetics Cell Biology Proteomics Life sciences Human genetics Biochemistry Cell biology |
| url | https://doi.org/10.1007/978-1-59745-388-2 |
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