Plant Virology Protocols: From Virus Isolation to Transgenic Resistance
Plant Virology Protocols offers for the first time a comprehensive collection of state-of-the-art techniques for generating transgenic plants that are resistant to plant viruses via the cloning and expression of the coat protein gene. Its unfailingly reproducible methods, perfected by hands-on maste...
Gespeichert in:
| Weitere Verfasser: | , |
|---|---|
| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
Totowa, NJ
Humana Press
1998
|
| Schriftenreihe: | Methods in Molecular Biology™
81 |
| Schlagworte: | |
| Online-Zugang: | DE-355 Volltext |
| Zusammenfassung: | Plant Virology Protocols offers for the first time a comprehensive collection of state-of-the-art techniques for generating transgenic plants that are resistant to plant viruses via the cloning and expression of the coat protein gene. Its unfailingly reproducible methods, perfected by hands-on masters, cover the entire process from virus isolation, RNA extraction, and cloning coat protein genes, to the introduction of the coat protein gene into the plant genome and the testing of transgenic plants for resistance. Methods for testing for transformation by PCR and Southern blotting, the detection of RNA transcripts by Northern blotting, and the production of protein by Western analysis are provided, as are methods for challenging the transgenic plants produced and for detecting and measuring the levels of virus. The authoritative contributors also discuss the history and mechanisms of coat protein-mediated protection and the key ethical issues surrounding transgenic technology. Unprecedented in its comprehensiveness and its many time-proven methods, Plant Virology Protocols is certain to become the authoritative standard reference for plant scientists who want to tap the enormous benefits this technology has to offer |
| Beschreibung: | 1 Online-Ressource (XV, 571 p. 111 illus) |
| ISBN: | 9781592595662 |
| DOI: | 10.1385/0896033856 |
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Datensatz im Suchindex
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| adam_text | |
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| author2 | Foster, Gary D. Taylor, Sally C. |
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| dewey-full | 580 579.2/8 |
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| dewey-search | 580 579.2/8 |
| dewey-sort | 3580 |
| dewey-tens | 580 - Plants 570 - Biology |
| discipline | Biologie Agrarwissenschaft Pflanzenbau |
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| spelling | Plant Virology Protocols From Virus Isolation to Transgenic Resistance edited by Gary D. Foster, Sally C. Taylor Totowa, NJ Humana Press 1998 1 Online-Ressource (XV, 571 p. 111 illus) txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology™ 81 Plant Virology Protocols offers for the first time a comprehensive collection of state-of-the-art techniques for generating transgenic plants that are resistant to plant viruses via the cloning and expression of the coat protein gene. Its unfailingly reproducible methods, perfected by hands-on masters, cover the entire process from virus isolation, RNA extraction, and cloning coat protein genes, to the introduction of the coat protein gene into the plant genome and the testing of transgenic plants for resistance. Methods for testing for transformation by PCR and Southern blotting, the detection of RNA transcripts by Northern blotting, and the production of protein by Western analysis are provided, as are methods for challenging the transgenic plants produced and for detecting and measuring the levels of virus. The authoritative contributors also discuss the history and mechanisms of coat protein-mediated protection and the key ethical issues surrounding transgenic technology. Unprecedented in its comprehensiveness and its many time-proven methods, Plant Virology Protocols is certain to become the authoritative standard reference for plant scientists who want to tap the enormous benefits this technology has to offer Life Sciences Plant Sciences Life sciences Plant science Botany Methode (DE-588)4038971-6 gnd rswk-swf Pflanzenviren (DE-588)4174074-9 gnd rswk-swf Virologie (DE-588)4063597-1 gnd rswk-swf Molekularbiologie (DE-588)4039983-7 gnd rswk-swf 1\p (DE-588)4143413-4 Aufsatzsammlung gnd-content Pflanzenviren (DE-588)4174074-9 s Virologie (DE-588)4063597-1 s Methode (DE-588)4038971-6 s DE-604 Molekularbiologie (DE-588)4039983-7 s Foster, Gary D. edt Taylor, Sally C. edt Erscheint auch als Druck-Ausgabe 9780896033856 https://doi.org/10.1385/0896033856 Verlag URL des Erstveröffentlichers Volltext 1\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk |
| spellingShingle | Plant Virology Protocols From Virus Isolation to Transgenic Resistance Life Sciences Plant Sciences Life sciences Plant science Botany Methode (DE-588)4038971-6 gnd Pflanzenviren (DE-588)4174074-9 gnd Virologie (DE-588)4063597-1 gnd Molekularbiologie (DE-588)4039983-7 gnd |
| subject_GND | (DE-588)4038971-6 (DE-588)4174074-9 (DE-588)4063597-1 (DE-588)4039983-7 (DE-588)4143413-4 |
| title | Plant Virology Protocols From Virus Isolation to Transgenic Resistance |
| title_auth | Plant Virology Protocols From Virus Isolation to Transgenic Resistance |
| title_exact_search | Plant Virology Protocols From Virus Isolation to Transgenic Resistance |
| title_full | Plant Virology Protocols From Virus Isolation to Transgenic Resistance edited by Gary D. Foster, Sally C. Taylor |
| title_fullStr | Plant Virology Protocols From Virus Isolation to Transgenic Resistance edited by Gary D. Foster, Sally C. Taylor |
| title_full_unstemmed | Plant Virology Protocols From Virus Isolation to Transgenic Resistance edited by Gary D. Foster, Sally C. Taylor |
| title_short | Plant Virology Protocols |
| title_sort | plant virology protocols from virus isolation to transgenic resistance |
| title_sub | From Virus Isolation to Transgenic Resistance |
| topic | Life Sciences Plant Sciences Life sciences Plant science Botany Methode (DE-588)4038971-6 gnd Pflanzenviren (DE-588)4174074-9 gnd Virologie (DE-588)4063597-1 gnd Molekularbiologie (DE-588)4039983-7 gnd |
| topic_facet | Life Sciences Plant Sciences Life sciences Plant science Botany Methode Pflanzenviren Virologie Molekularbiologie Aufsatzsammlung |
| url | https://doi.org/10.1385/0896033856 |
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