Methods in Biological Oxidative Stress:
Free radicals and reactive oxidizing agents are now recognized as causes or contributing factors in inflammation and other disease states and have become a major focus of interest in pharmacology, toxicology, physiology, and biochemistry. In Methods in Biological Oxidative Stress, expert researchers...
Gespeichert in:
| Weitere Verfasser: | , |
|---|---|
| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
Totowa, NJ
Humana Press
2003
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| Schriftenreihe: | Methods in Pharmacology and Toxicology
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| Schlagworte: | |
| Online-Zugang: | UBR01 TUM01 URL des Erstveröffentlichers |
| Zusammenfassung: | Free radicals and reactive oxidizing agents are now recognized as causes or contributing factors in inflammation and other disease states and have become a major focus of interest in pharmacology, toxicology, physiology, and biochemistry. In Methods in Biological Oxidative Stress, expert researchers detail their best experimental methods for detecting free radicals and reactive oxygen species or their byproducts. The techniques range from established standard protocols to advanced methodologies using HPLC, mass spectrometry, and electron paramagnetic resonance. The HPLC techniques are applied to the electrochemical measurement of protein oxidation products, particularly nitrotyrosine and dityrosine, and to the electrochemical detection of DNA oxidation products. There are also mass spectrometry methods for measuring lipid oxidation products. Each technique is described in step-by-step detail to ensure robust results, with many presented by the experimenters who have led their development. Useful notes explain the pitfalls to avoid and the chemical principles underlying each method. Authoritative and highly practical, Methods in Biological Oxidative Stress provides laboratory researchers and technicians a unique collection of standard, easy-to-use experimental methods for oxidative stress bioanalysis, plus cutting-edge instrumental techniques using HPLC, mass spectrometry, and EPR spectrometry |
| Beschreibung: | 1 Online-Ressource (XVI, 216 p) |
| ISBN: | 9781592594245 |
| DOI: | 10.1385/1592594247 |
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| 520 | |a Free radicals and reactive oxidizing agents are now recognized as causes or contributing factors in inflammation and other disease states and have become a major focus of interest in pharmacology, toxicology, physiology, and biochemistry. In Methods in Biological Oxidative Stress, expert researchers detail their best experimental methods for detecting free radicals and reactive oxygen species or their byproducts. The techniques range from established standard protocols to advanced methodologies using HPLC, mass spectrometry, and electron paramagnetic resonance. The HPLC techniques are applied to the electrochemical measurement of protein oxidation products, particularly nitrotyrosine and dityrosine, and to the electrochemical detection of DNA oxidation products. There are also mass spectrometry methods for measuring lipid oxidation products. Each technique is described in step-by-step detail to ensure robust results, with many presented by the experimenters who have led their development. Useful notes explain the pitfalls to avoid and the chemical principles underlying each method. Authoritative and highly practical, Methods in Biological Oxidative Stress provides laboratory researchers and technicians a unique collection of standard, easy-to-use experimental methods for oxidative stress bioanalysis, plus cutting-edge instrumental techniques using HPLC, mass spectrometry, and EPR spectrometry | ||
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| dewey-ones | 615 - Pharmacology and therapeutics |
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| discipline | Medizin |
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| spelling | Methods in Biological Oxidative Stress edited by Kenneth Hensley, Robert A. Floyd Totowa, NJ Humana Press 2003 1 Online-Ressource (XVI, 216 p) txt rdacontent c rdamedia cr rdacarrier Methods in Pharmacology and Toxicology Free radicals and reactive oxidizing agents are now recognized as causes or contributing factors in inflammation and other disease states and have become a major focus of interest in pharmacology, toxicology, physiology, and biochemistry. In Methods in Biological Oxidative Stress, expert researchers detail their best experimental methods for detecting free radicals and reactive oxygen species or their byproducts. The techniques range from established standard protocols to advanced methodologies using HPLC, mass spectrometry, and electron paramagnetic resonance. The HPLC techniques are applied to the electrochemical measurement of protein oxidation products, particularly nitrotyrosine and dityrosine, and to the electrochemical detection of DNA oxidation products. There are also mass spectrometry methods for measuring lipid oxidation products. Each technique is described in step-by-step detail to ensure robust results, with many presented by the experimenters who have led their development. Useful notes explain the pitfalls to avoid and the chemical principles underlying each method. Authoritative and highly practical, Methods in Biological Oxidative Stress provides laboratory researchers and technicians a unique collection of standard, easy-to-use experimental methods for oxidative stress bioanalysis, plus cutting-edge instrumental techniques using HPLC, mass spectrometry, and EPR spectrometry Biomedicine Pharmacology/Toxicology Medicine Pharmacology Hensley, Kenneth edt Floyd, Robert A. edt Erscheint auch als Druck-Ausgabe 9780896038158 https://doi.org/10.1385/1592594247 Verlag URL des Erstveröffentlichers Volltext |
| spellingShingle | Methods in Biological Oxidative Stress Biomedicine Pharmacology/Toxicology Medicine Pharmacology |
| title | Methods in Biological Oxidative Stress |
| title_auth | Methods in Biological Oxidative Stress |
| title_exact_search | Methods in Biological Oxidative Stress |
| title_full | Methods in Biological Oxidative Stress edited by Kenneth Hensley, Robert A. Floyd |
| title_fullStr | Methods in Biological Oxidative Stress edited by Kenneth Hensley, Robert A. Floyd |
| title_full_unstemmed | Methods in Biological Oxidative Stress edited by Kenneth Hensley, Robert A. Floyd |
| title_short | Methods in Biological Oxidative Stress |
| title_sort | methods in biological oxidative stress |
| topic | Biomedicine Pharmacology/Toxicology Medicine Pharmacology |
| topic_facet | Biomedicine Pharmacology/Toxicology Medicine Pharmacology |
| url | https://doi.org/10.1385/1592594247 |
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