2D PAGE: Sample Preparation and Fractionation:
The complex nature of proteins often requires a multitude of sample preparation options. In 2D PAGE: Sample Preparation and Fractionation, Volumes 1 & 2, Dr. Anton Posch presents a broad coverage of the principles and recent developments of sample preparation and fractionation tools in Expressio...
Gespeichert in:
| Weitere Verfasser: | |
|---|---|
| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
Totowa, NJ
Humana Press
2008
|
| Schriftenreihe: | Methods in Molecular Biology™
424 |
| Schlagworte: | |
| Online-Zugang: | UBR01 TUM01 Volltext |
| Zusammenfassung: | The complex nature of proteins often requires a multitude of sample preparation options. In 2D PAGE: Sample Preparation and Fractionation, Volumes 1 & 2, Dr. Anton Posch presents a broad coverage of the principles and recent developments of sample preparation and fractionation tools in Expression Proteomics and specifically for two-dimensional electrophoresis (2-DE). Detailed laboratory methods describe the process of sample fractionation used to reduce the complexity of the protein sample and the methods of 2-DE analysis and a broad variety of gel-free mass spectrometry-based technologies to detect previously undetectable proteins. Vital information regarding protein isolation and preparation for reproducible proteome analysis previously overlooked by scientists has been clearly laid out in this pioneering two-volume set. Volume 1 focuses on the basics of sample preparation and protein labeling techniques with an emphasis on fractionation by chemical reagents, chromatography, and electrophoresis methods. The inclusion of meticulous enrichment strategies further aids the protein researcher. Invaluable for students of Biochemistry, Biomedicine, Biology and Genomics and essential for the practicing scientists, 2D PAGE: Sample Preparation and Fractionation, Volumes 1 & 2 delve into the study of proteins with comprehensive and in depth methods and protocols to provide a greater understanding of this intricate and complicated field |
| Beschreibung: | 1 Online-Ressource (XIII, 459 p. 133 illus., 2 illus. in color) |
| ISBN: | 9781603270649 |
| DOI: | 10.1007/978-1-60327-064-9 |
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| spelling | 2D PAGE: Sample Preparation and Fractionation edited by Anton Posch Totowa, NJ Humana Press 2008 1 Online-Ressource (XIII, 459 p. 133 illus., 2 illus. in color) txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology™ 424 The complex nature of proteins often requires a multitude of sample preparation options. In 2D PAGE: Sample Preparation and Fractionation, Volumes 1 & 2, Dr. Anton Posch presents a broad coverage of the principles and recent developments of sample preparation and fractionation tools in Expression Proteomics and specifically for two-dimensional electrophoresis (2-DE). Detailed laboratory methods describe the process of sample fractionation used to reduce the complexity of the protein sample and the methods of 2-DE analysis and a broad variety of gel-free mass spectrometry-based technologies to detect previously undetectable proteins. Vital information regarding protein isolation and preparation for reproducible proteome analysis previously overlooked by scientists has been clearly laid out in this pioneering two-volume set. Volume 1 focuses on the basics of sample preparation and protein labeling techniques with an emphasis on fractionation by chemical reagents, chromatography, and electrophoresis methods. The inclusion of meticulous enrichment strategies further aids the protein researcher. Invaluable for students of Biochemistry, Biomedicine, Biology and Genomics and essential for the practicing scientists, 2D PAGE: Sample Preparation and Fractionation, Volumes 1 & 2 delve into the study of proteins with comprehensive and in depth methods and protocols to provide a greater understanding of this intricate and complicated field Life Sciences Proteomics Cell Biology Biochemistry, general Microbial Genetics and Genomics Life sciences Biochemistry Cell biology Microbial genetics Microbial genomics Zweidimensionale Elektrophorese (DE-588)4221393-9 gnd rswk-swf Zweidimensionale Elektrophorese (DE-588)4221393-9 s b DE-604 Posch, Anton edt Erscheint auch als Druck-Ausgabe 9781588297228 https://doi.org/10.1007/978-1-60327-064-9 Verlag URL des Erstveröffentlichers Volltext |
| spellingShingle | 2D PAGE: Sample Preparation and Fractionation Life Sciences Proteomics Cell Biology Biochemistry, general Microbial Genetics and Genomics Life sciences Biochemistry Cell biology Microbial genetics Microbial genomics Zweidimensionale Elektrophorese (DE-588)4221393-9 gnd |
| subject_GND | (DE-588)4221393-9 |
| title | 2D PAGE: Sample Preparation and Fractionation |
| title_auth | 2D PAGE: Sample Preparation and Fractionation |
| title_exact_search | 2D PAGE: Sample Preparation and Fractionation |
| title_full | 2D PAGE: Sample Preparation and Fractionation edited by Anton Posch |
| title_fullStr | 2D PAGE: Sample Preparation and Fractionation edited by Anton Posch |
| title_full_unstemmed | 2D PAGE: Sample Preparation and Fractionation edited by Anton Posch |
| title_short | 2D PAGE: Sample Preparation and Fractionation |
| title_sort | 2d page sample preparation and fractionation |
| topic | Life Sciences Proteomics Cell Biology Biochemistry, general Microbial Genetics and Genomics Life sciences Biochemistry Cell biology Microbial genetics Microbial genomics Zweidimensionale Elektrophorese (DE-588)4221393-9 gnd |
| topic_facet | Life Sciences Proteomics Cell Biology Biochemistry, general Microbial Genetics and Genomics Life sciences Biochemistry Cell biology Microbial genetics Microbial genomics Zweidimensionale Elektrophorese |
| url | https://doi.org/10.1007/978-1-60327-064-9 |
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