Chromatin Protocols:
Updated and revised, this thorough volume is organized such that it begins with techniques related to the study of chromatin structure. Protocols for reconstitution of chromatin on solid supports for analysis, preparation of positioned mononucleosomes, techniques to study premature chromatin condens...
Gespeichert in:
| Weitere Verfasser: | |
|---|---|
| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
New York, NY
Springer New York
2015
|
| Ausgabe: | 3rd ed. 2015 |
| Schriftenreihe: | Methods in Molecular Biology
1288 |
| Schlagworte: | |
| Online-Zugang: | UBR01 TUM01 Volltext |
| Zusammenfassung: | Updated and revised, this thorough volume is organized such that it begins with techniques related to the study of chromatin structure. Protocols for reconstitution of chromatin on solid supports for analysis, preparation of positioned mononucleosomes, techniques to study premature chromatin condensation, and the use of comparative genomic hybridization to assess genomic aberration are included as well. Novel techniques for imaging chromatin using atomic force microscopy and the isolation of specific genomic regions using engineered DNA binding molecules generated by CRISPR are then examined. That section is followed by protocols to analyze DNA and histone modifications, while the third section includes methods to study DNA replication and repair, in the context of chromatin. Last but not least, protocols for studying chromatin and its relation with transcriptional regulation are presented in a fourth section. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Chromatin Protocols, Third Edition aims to help researchers in facilitating in-depth molecular analysis of various aspects of chromatin structure and function |
| Beschreibung: | 1 Online-Ressource (XIV, 492 p. 74 illus., 36 illus. in color) |
| ISBN: | 9781493924745 |
| DOI: | 10.1007/978-1-4939-2474-5 |
Internformat
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Datensatz im Suchindex
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| discipline | Biologie |
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| edition | 3rd ed. 2015 |
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| spelling | Chromatin Protocols edited by Srikumar P. Chellappan 3rd ed. 2015 New York, NY Springer New York 2015 1 Online-Ressource (XIV, 492 p. 74 illus., 36 illus. in color) txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology 1288 Updated and revised, this thorough volume is organized such that it begins with techniques related to the study of chromatin structure. Protocols for reconstitution of chromatin on solid supports for analysis, preparation of positioned mononucleosomes, techniques to study premature chromatin condensation, and the use of comparative genomic hybridization to assess genomic aberration are included as well. Novel techniques for imaging chromatin using atomic force microscopy and the isolation of specific genomic regions using engineered DNA binding molecules generated by CRISPR are then examined. That section is followed by protocols to analyze DNA and histone modifications, while the third section includes methods to study DNA replication and repair, in the context of chromatin. Last but not least, protocols for studying chromatin and its relation with transcriptional regulation are presented in a fourth section. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Chromatin Protocols, Third Edition aims to help researchers in facilitating in-depth molecular analysis of various aspects of chromatin structure and function Life Sciences Cell Biology Life sciences Cell biology Chromatin (DE-588)4010152-6 gnd rswk-swf Eukaryontische Zelle (DE-588)4153142-5 gnd rswk-swf Molekularbiologie (DE-588)4039983-7 gnd rswk-swf 1\p (DE-588)4143413-4 Aufsatzsammlung gnd-content Chromatin (DE-588)4010152-6 s Molekularbiologie (DE-588)4039983-7 s Eukaryontische Zelle (DE-588)4153142-5 s 2\p DE-604 Chellappan, Srikumar P. edt Erscheint auch als Druck-Ausgabe 9781493924738 https://doi.org/10.1007/978-1-4939-2474-5 Verlag URL des Erstveröffentlichers Volltext 1\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk 2\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk |
| spellingShingle | Chromatin Protocols Life Sciences Cell Biology Life sciences Cell biology Chromatin (DE-588)4010152-6 gnd Eukaryontische Zelle (DE-588)4153142-5 gnd Molekularbiologie (DE-588)4039983-7 gnd |
| subject_GND | (DE-588)4010152-6 (DE-588)4153142-5 (DE-588)4039983-7 (DE-588)4143413-4 |
| title | Chromatin Protocols |
| title_auth | Chromatin Protocols |
| title_exact_search | Chromatin Protocols |
| title_full | Chromatin Protocols edited by Srikumar P. Chellappan |
| title_fullStr | Chromatin Protocols edited by Srikumar P. Chellappan |
| title_full_unstemmed | Chromatin Protocols edited by Srikumar P. Chellappan |
| title_short | Chromatin Protocols |
| title_sort | chromatin protocols |
| topic | Life Sciences Cell Biology Life sciences Cell biology Chromatin (DE-588)4010152-6 gnd Eukaryontische Zelle (DE-588)4153142-5 gnd Molekularbiologie (DE-588)4039983-7 gnd |
| topic_facet | Life Sciences Cell Biology Life sciences Cell biology Chromatin Eukaryontische Zelle Molekularbiologie Aufsatzsammlung |
| url | https://doi.org/10.1007/978-1-4939-2474-5 |
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