Engineered Zinc Finger Proteins: Methods and Protocols
Among the many types of DNA binding domains, C2H2 zinc finger proteins (ZFPs) have proven to be the most malleable for creating custom DNA-binding proteins. In Engineered Zinc Finger Proteins: Methods and Protocols, expert researchers from some of the most active laboratories in this field present d...
Gespeichert in:
| Weitere Verfasser: | , |
|---|---|
| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
Totowa, NJ
Humana Press
2010
|
| Ausgabe: | 1st |
| Schriftenreihe: | Methods in Molecular Biology, Methods and Protocols
649 |
| Schlagworte: | |
| Online-Zugang: | UBR01 TUM01 URL des Erstveröffentlichers |
| Zusammenfassung: | Among the many types of DNA binding domains, C2H2 zinc finger proteins (ZFPs) have proven to be the most malleable for creating custom DNA-binding proteins. In Engineered Zinc Finger Proteins: Methods and Protocols, expert researchers from some of the most active laboratories in this field present detailed methods, guidance, and perspectives. The volume contains sections covering the engineering of ZFPs, methods for the creation, evaluation, and delivery of artificial transcription factors (ATFs), methods for the creation and evaluation of zinc finger nucleases (ZFNs), and a collection of the several applications and assays beyond ATFs and ZFNs, including zinc finger transposases and ChIP-seq methodology amongst other subjects. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Engineered Zinc Finger Proteins: Methods and Protocols aims to aid both seasoned practitioners and new investigators with its vital methods and insights as they seek to create the next generation of engineered ZFPs and applications |
| Beschreibung: | 1 Online-Ressource (XIV, 440 p) |
| ISBN: | 9781607617532 |
| DOI: | 10.1007/978-1-60761-753-2 |
Internformat
MARC
| LEADER | 00000nmm a2200000zcb4500 | ||
|---|---|---|---|
| 001 | BV044950662 | ||
| 003 | DE-604 | ||
| 005 | 00000000000000.0 | ||
| 007 | cr|uuu---uuuuu | ||
| 008 | 180517s2010 |||| o||u| ||||||eng d | ||
| 020 | |a 9781607617532 |9 978-1-60761-753-2 | ||
| 024 | 7 | |a 10.1007/978-1-60761-753-2 |2 doi | |
| 035 | |a (ZDB-2-PRO)978-1-60761-753-2 | ||
| 035 | |a (OCoLC)891106197 | ||
| 035 | |a (DE-599)BVBBV044950662 | ||
| 040 | |a DE-604 |b ger |e rda | ||
| 041 | 0 | |a eng | |
| 049 | |a DE-355 |a DE-91 | ||
| 082 | 0 | |a 572.6 |2 23 | |
| 084 | |a WC 4170 |0 (DE-625)148093: |2 rvk | ||
| 245 | 1 | 0 | |a Engineered Zinc Finger Proteins |b Methods and Protocols |c edited by Joel P. Mackay, David J. Segal |
| 250 | |a 1st | ||
| 264 | 1 | |a Totowa, NJ |b Humana Press |c 2010 | |
| 300 | |a 1 Online-Ressource (XIV, 440 p) | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b c |2 rdamedia | ||
| 338 | |b cr |2 rdacarrier | ||
| 490 | 0 | |a Methods in Molecular Biology, Methods and Protocols |v 649 | |
| 520 | |a Among the many types of DNA binding domains, C2H2 zinc finger proteins (ZFPs) have proven to be the most malleable for creating custom DNA-binding proteins. In Engineered Zinc Finger Proteins: Methods and Protocols, expert researchers from some of the most active laboratories in this field present detailed methods, guidance, and perspectives. The volume contains sections covering the engineering of ZFPs, methods for the creation, evaluation, and delivery of artificial transcription factors (ATFs), methods for the creation and evaluation of zinc finger nucleases (ZFNs), and a collection of the several applications and assays beyond ATFs and ZFNs, including zinc finger transposases and ChIP-seq methodology amongst other subjects. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Engineered Zinc Finger Proteins: Methods and Protocols aims to aid both seasoned practitioners and new investigators with its vital methods and insights as they seek to create the next generation of engineered ZFPs and applications | ||
| 650 | 4 | |a Life Sciences | |
| 650 | 4 | |a Protein Science | |
| 650 | 4 | |a Genetic Engineering | |
| 650 | 4 | |a Life sciences | |
| 650 | 4 | |a Genetic engineering | |
| 650 | 4 | |a Proteins | |
| 650 | 0 | 7 | |a Zink-Finger-Proteine |0 (DE-588)4326267-3 |2 gnd |9 rswk-swf |
| 655 | 7 | |8 1\p |0 (DE-588)4143413-4 |a Aufsatzsammlung |2 gnd-content | |
| 689 | 0 | 0 | |a Zink-Finger-Proteine |0 (DE-588)4326267-3 |D s |
| 689 | 0 | |8 2\p |5 DE-604 | |
| 700 | 1 | |a Mackay, Joel P. |4 edt | |
| 700 | 1 | |a Segal, David J. |4 edt | |
| 776 | 0 | 8 | |i Erscheint auch als |n Druck-Ausgabe |z 9781607617525 |
| 856 | 4 | 0 | |u https://doi.org/10.1007/978-1-60761-753-2 |x Verlag |z URL des Erstveröffentlichers |3 Volltext |
| 912 | |a ZDB-2-PRO | ||
| 999 | |a oai:aleph.bib-bvb.de:BVB01-030343422 | ||
| 883 | 1 | |8 1\p |a cgwrk |d 20201028 |q DE-101 |u https://d-nb.info/provenance/plan#cgwrk | |
| 883 | 1 | |8 2\p |a cgwrk |d 20201028 |q DE-101 |u https://d-nb.info/provenance/plan#cgwrk | |
| 966 | e | |u https://doi.org/10.1007/978-1-60761-753-2 |l UBR01 |p ZDB-2-PRO |x Verlag |3 Volltext | |
| 966 | e | |u https://doi.org/10.1007/978-1-60761-753-2 |l TUM01 |p ZDB-2-PRO |x Verlag |3 Volltext | |
Datensatz im Suchindex
| _version_ | 1804178538313547776 |
|---|---|
| any_adam_object | |
| author2 | Mackay, Joel P. Segal, David J. |
| author2_role | edt edt |
| author2_variant | j p m jp jpm d j s dj djs |
| author_facet | Mackay, Joel P. Segal, David J. |
| building | Verbundindex |
| bvnumber | BV044950662 |
| classification_rvk | WC 4170 |
| collection | ZDB-2-PRO |
| ctrlnum | (ZDB-2-PRO)978-1-60761-753-2 (OCoLC)891106197 (DE-599)BVBBV044950662 |
| dewey-full | 572.6 |
| dewey-hundreds | 500 - Natural sciences and mathematics |
| dewey-ones | 572 - Biochemistry |
| dewey-raw | 572.6 |
| dewey-search | 572.6 |
| dewey-sort | 3572.6 |
| dewey-tens | 570 - Biology |
| discipline | Biologie |
| doi_str_mv | 10.1007/978-1-60761-753-2 |
| edition | 1st |
| format | Electronic eBook |
| fullrecord | <?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>03358nmm a2200553zcb4500</leader><controlfield tag="001">BV044950662</controlfield><controlfield tag="003">DE-604</controlfield><controlfield tag="005">00000000000000.0</controlfield><controlfield tag="007">cr|uuu---uuuuu</controlfield><controlfield tag="008">180517s2010 |||| o||u| ||||||eng d</controlfield><datafield tag="020" ind1=" " ind2=" "><subfield code="a">9781607617532</subfield><subfield code="9">978-1-60761-753-2</subfield></datafield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1007/978-1-60761-753-2</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(ZDB-2-PRO)978-1-60761-753-2</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(OCoLC)891106197</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)BVBBV044950662</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-604</subfield><subfield code="b">ger</subfield><subfield code="e">rda</subfield></datafield><datafield tag="041" ind1="0" ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="049" ind1=" " ind2=" "><subfield code="a">DE-355</subfield><subfield code="a">DE-91</subfield></datafield><datafield tag="082" ind1="0" ind2=" "><subfield code="a">572.6</subfield><subfield code="2">23</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">WC 4170</subfield><subfield code="0">(DE-625)148093:</subfield><subfield code="2">rvk</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Engineered Zinc Finger Proteins</subfield><subfield code="b">Methods and Protocols</subfield><subfield code="c">edited by Joel P. Mackay, David J. Segal</subfield></datafield><datafield tag="250" ind1=" " ind2=" "><subfield code="a">1st</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="a">Totowa, NJ</subfield><subfield code="b">Humana Press</subfield><subfield code="c">2010</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">1 Online-Ressource (XIV, 440 p)</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="490" ind1="0" ind2=" "><subfield code="a">Methods in Molecular Biology, Methods and Protocols</subfield><subfield code="v">649</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Among the many types of DNA binding domains, C2H2 zinc finger proteins (ZFPs) have proven to be the most malleable for creating custom DNA-binding proteins. In Engineered Zinc Finger Proteins: Methods and Protocols, expert researchers from some of the most active laboratories in this field present detailed methods, guidance, and perspectives. The volume contains sections covering the engineering of ZFPs, methods for the creation, evaluation, and delivery of artificial transcription factors (ATFs), methods for the creation and evaluation of zinc finger nucleases (ZFNs), and a collection of the several applications and assays beyond ATFs and ZFNs, including zinc finger transposases and ChIP-seq methodology amongst other subjects. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Engineered Zinc Finger Proteins: Methods and Protocols aims to aid both seasoned practitioners and new investigators with its vital methods and insights as they seek to create the next generation of engineered ZFPs and applications</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Life Sciences</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Protein Science</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Genetic Engineering</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Life sciences</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Genetic engineering</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Proteins</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Zink-Finger-Proteine</subfield><subfield code="0">(DE-588)4326267-3</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="655" ind1=" " ind2="7"><subfield code="8">1\p</subfield><subfield code="0">(DE-588)4143413-4</subfield><subfield code="a">Aufsatzsammlung</subfield><subfield code="2">gnd-content</subfield></datafield><datafield tag="689" ind1="0" ind2="0"><subfield code="a">Zink-Finger-Proteine</subfield><subfield code="0">(DE-588)4326267-3</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2=" "><subfield code="8">2\p</subfield><subfield code="5">DE-604</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Mackay, Joel P.</subfield><subfield code="4">edt</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Segal, David J.</subfield><subfield code="4">edt</subfield></datafield><datafield tag="776" ind1="0" ind2="8"><subfield code="i">Erscheint auch als</subfield><subfield code="n">Druck-Ausgabe</subfield><subfield code="z">9781607617525</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://doi.org/10.1007/978-1-60761-753-2</subfield><subfield code="x">Verlag</subfield><subfield code="z">URL des Erstveröffentlichers</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">ZDB-2-PRO</subfield></datafield><datafield tag="999" ind1=" " ind2=" "><subfield code="a">oai:aleph.bib-bvb.de:BVB01-030343422</subfield></datafield><datafield tag="883" ind1="1" ind2=" "><subfield code="8">1\p</subfield><subfield code="a">cgwrk</subfield><subfield code="d">20201028</subfield><subfield code="q">DE-101</subfield><subfield code="u">https://d-nb.info/provenance/plan#cgwrk</subfield></datafield><datafield tag="883" ind1="1" ind2=" "><subfield code="8">2\p</subfield><subfield code="a">cgwrk</subfield><subfield code="d">20201028</subfield><subfield code="q">DE-101</subfield><subfield code="u">https://d-nb.info/provenance/plan#cgwrk</subfield></datafield><datafield tag="966" ind1="e" ind2=" "><subfield code="u">https://doi.org/10.1007/978-1-60761-753-2</subfield><subfield code="l">UBR01</subfield><subfield code="p">ZDB-2-PRO</subfield><subfield code="x">Verlag</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="966" ind1="e" ind2=" "><subfield code="u">https://doi.org/10.1007/978-1-60761-753-2</subfield><subfield code="l">TUM01</subfield><subfield code="p">ZDB-2-PRO</subfield><subfield code="x">Verlag</subfield><subfield code="3">Volltext</subfield></datafield></record></collection> |
| genre | 1\p (DE-588)4143413-4 Aufsatzsammlung gnd-content |
| genre_facet | Aufsatzsammlung |
| id | DE-604.BV044950662 |
| illustrated | Not Illustrated |
| indexdate | 2024-07-10T08:05:34Z |
| institution | BVB |
| isbn | 9781607617532 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-030343422 |
| oclc_num | 891106197 |
| open_access_boolean | |
| owner | DE-355 DE-BY-UBR DE-91 DE-BY-TUM |
| owner_facet | DE-355 DE-BY-UBR DE-91 DE-BY-TUM |
| physical | 1 Online-Ressource (XIV, 440 p) |
| psigel | ZDB-2-PRO |
| publishDate | 2010 |
| publishDateSearch | 2010 |
| publishDateSort | 2010 |
| publisher | Humana Press |
| record_format | marc |
| series2 | Methods in Molecular Biology, Methods and Protocols |
| spelling | Engineered Zinc Finger Proteins Methods and Protocols edited by Joel P. Mackay, David J. Segal 1st Totowa, NJ Humana Press 2010 1 Online-Ressource (XIV, 440 p) txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology, Methods and Protocols 649 Among the many types of DNA binding domains, C2H2 zinc finger proteins (ZFPs) have proven to be the most malleable for creating custom DNA-binding proteins. In Engineered Zinc Finger Proteins: Methods and Protocols, expert researchers from some of the most active laboratories in this field present detailed methods, guidance, and perspectives. The volume contains sections covering the engineering of ZFPs, methods for the creation, evaluation, and delivery of artificial transcription factors (ATFs), methods for the creation and evaluation of zinc finger nucleases (ZFNs), and a collection of the several applications and assays beyond ATFs and ZFNs, including zinc finger transposases and ChIP-seq methodology amongst other subjects. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Engineered Zinc Finger Proteins: Methods and Protocols aims to aid both seasoned practitioners and new investigators with its vital methods and insights as they seek to create the next generation of engineered ZFPs and applications Life Sciences Protein Science Genetic Engineering Life sciences Genetic engineering Proteins Zink-Finger-Proteine (DE-588)4326267-3 gnd rswk-swf 1\p (DE-588)4143413-4 Aufsatzsammlung gnd-content Zink-Finger-Proteine (DE-588)4326267-3 s 2\p DE-604 Mackay, Joel P. edt Segal, David J. edt Erscheint auch als Druck-Ausgabe 9781607617525 https://doi.org/10.1007/978-1-60761-753-2 Verlag URL des Erstveröffentlichers Volltext 1\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk 2\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk |
| spellingShingle | Engineered Zinc Finger Proteins Methods and Protocols Life Sciences Protein Science Genetic Engineering Life sciences Genetic engineering Proteins Zink-Finger-Proteine (DE-588)4326267-3 gnd |
| subject_GND | (DE-588)4326267-3 (DE-588)4143413-4 |
| title | Engineered Zinc Finger Proteins Methods and Protocols |
| title_auth | Engineered Zinc Finger Proteins Methods and Protocols |
| title_exact_search | Engineered Zinc Finger Proteins Methods and Protocols |
| title_full | Engineered Zinc Finger Proteins Methods and Protocols edited by Joel P. Mackay, David J. Segal |
| title_fullStr | Engineered Zinc Finger Proteins Methods and Protocols edited by Joel P. Mackay, David J. Segal |
| title_full_unstemmed | Engineered Zinc Finger Proteins Methods and Protocols edited by Joel P. Mackay, David J. Segal |
| title_short | Engineered Zinc Finger Proteins |
| title_sort | engineered zinc finger proteins methods and protocols |
| title_sub | Methods and Protocols |
| topic | Life Sciences Protein Science Genetic Engineering Life sciences Genetic engineering Proteins Zink-Finger-Proteine (DE-588)4326267-3 gnd |
| topic_facet | Life Sciences Protein Science Genetic Engineering Life sciences Genetic engineering Proteins Zink-Finger-Proteine Aufsatzsammlung |
| url | https://doi.org/10.1007/978-1-60761-753-2 |
| work_keys_str_mv | AT mackayjoelp engineeredzincfingerproteinsmethodsandprotocols AT segaldavidj engineeredzincfingerproteinsmethodsandprotocols |