Poly(ADP-Ribose) Polymerase: Methods and Protocols
This book presents multiple new and classical methods for studying the vital poly-ADP-ribose (pADPr) pathway. Beginning with techniques for the detection and quantification of the product of poly(ADP-ribose) polymerase (PARP) enzymatic activity and detection of variation in pADPr production during t...
Gespeichert in:
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| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
New York, NY
Springer New York
2017
|
| Ausgabe: | 2nd ed. 2017 |
| Schriftenreihe: | Methods in Molecular Biology
1608 |
| Schlagworte: | |
| Online-Zugang: | UBR01 TUM01 Volltext |
| Zusammenfassung: | This book presents multiple new and classical methods for studying the vital poly-ADP-ribose (pADPr) pathway. Beginning with techniques for the detection and quantification of the product of poly(ADP-ribose) polymerase (PARP) enzymatic activity and detection of variation in pADPr production during the cell cycle, the volume continues with sections on the identification of pADPr protein acceptors, methods focusing on studying molecular mechanisms of PARP functions in eukaryotic cells, particularly those involved in control of DNA repair and oxidative stress, as well as in expression regulation, approaches to the in vitro reconstitution of PARP-1 interaction with chromatin, the development and testing of small molecule PARP inhibitors, and the functions of understudied members of PARP family. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Poly(ADP-Ribose) Polymerase: Methods and Protocols, Second Edition serves as an ideal companion to the first edition for scientists whose investigations involve this important pathway. The chapter 'Identifying and Validating Tankyrase Binders and Substrates: A Candidate Approach' is published open access under a CC BY 4.0 license |
| Beschreibung: | 1 Online-Ressource (XV, 528 p. 82 illus., 47 illus. in color) |
| ISBN: | 9781493969937 |
| DOI: | 10.1007/978-1-4939-6993-7 |
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| spelling | Poly(ADP-Ribose) Polymerase Methods and Protocols edited by Alexei V. Tulin 2nd ed. 2017 New York, NY Springer New York 2017 1 Online-Ressource (XV, 528 p. 82 illus., 47 illus. in color) txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology 1608 This book presents multiple new and classical methods for studying the vital poly-ADP-ribose (pADPr) pathway. Beginning with techniques for the detection and quantification of the product of poly(ADP-ribose) polymerase (PARP) enzymatic activity and detection of variation in pADPr production during the cell cycle, the volume continues with sections on the identification of pADPr protein acceptors, methods focusing on studying molecular mechanisms of PARP functions in eukaryotic cells, particularly those involved in control of DNA repair and oxidative stress, as well as in expression regulation, approaches to the in vitro reconstitution of PARP-1 interaction with chromatin, the development and testing of small molecule PARP inhibitors, and the functions of understudied members of PARP family. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Poly(ADP-Ribose) Polymerase: Methods and Protocols, Second Edition serves as an ideal companion to the first edition for scientists whose investigations involve this important pathway. The chapter 'Identifying and Validating Tankyrase Binders and Substrates: A Candidate Approach' is published open access under a CC BY 4.0 license Life Sciences Protein Science Life sciences Proteins Methode (DE-588)4038971-6 gnd rswk-swf NAD-ADP-Ribosyltransferase (DE-588)4313462-2 gnd rswk-swf 1\p (DE-588)4143413-4 Aufsatzsammlung gnd-content NAD-ADP-Ribosyltransferase (DE-588)4313462-2 s Methode (DE-588)4038971-6 s 2\p DE-604 Tulin, Alexei V. edt Erscheint auch als Druck-Ausgabe 9781493969920 https://doi.org/10.1007/978-1-4939-6993-7 Verlag URL des Erstveröffentlichers Volltext 1\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk 2\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk |
| spellingShingle | Poly(ADP-Ribose) Polymerase Methods and Protocols Life Sciences Protein Science Life sciences Proteins Methode (DE-588)4038971-6 gnd NAD-ADP-Ribosyltransferase (DE-588)4313462-2 gnd |
| subject_GND | (DE-588)4038971-6 (DE-588)4313462-2 (DE-588)4143413-4 |
| title | Poly(ADP-Ribose) Polymerase Methods and Protocols |
| title_auth | Poly(ADP-Ribose) Polymerase Methods and Protocols |
| title_exact_search | Poly(ADP-Ribose) Polymerase Methods and Protocols |
| title_full | Poly(ADP-Ribose) Polymerase Methods and Protocols edited by Alexei V. Tulin |
| title_fullStr | Poly(ADP-Ribose) Polymerase Methods and Protocols edited by Alexei V. Tulin |
| title_full_unstemmed | Poly(ADP-Ribose) Polymerase Methods and Protocols edited by Alexei V. Tulin |
| title_short | Poly(ADP-Ribose) Polymerase |
| title_sort | poly adp ribose polymerase methods and protocols |
| title_sub | Methods and Protocols |
| topic | Life Sciences Protein Science Life sciences Proteins Methode (DE-588)4038971-6 gnd NAD-ADP-Ribosyltransferase (DE-588)4313462-2 gnd |
| topic_facet | Life Sciences Protein Science Life sciences Proteins Methode NAD-ADP-Ribosyltransferase Aufsatzsammlung |
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