Zygotic Genome Activation: Methods and Protocols
This volume provides an overview of zygotic genome activation (ZGA) and the use of recent tools to elucidate the events that occur during ZGA. The chapters in this book cover topics such as: clearance of maternal RNAs, detection of miRNA in mammalian oocytes and embryos, RNA FISH to study ZGA in ear...
Gespeichert in:
| Weitere Verfasser: | |
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| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
New York, NY
Springer New York
2017
|
| Schriftenreihe: | Methods in Molecular Biology
1605 |
| Schlagworte: | |
| Online-Zugang: | UBR01 TUM01 URL des Erstveröffentlichers |
| Zusammenfassung: | This volume provides an overview of zygotic genome activation (ZGA) and the use of recent tools to elucidate the events that occur during ZGA. The chapters in this book cover topics such as: clearance of maternal RNAs, detection of miRNA in mammalian oocytes and embryos, RNA FISH to study ZGA in early mouse embryos, and CRISPR/Cas9-mediated gene targeting during embryogenesis in the mouse and pig. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and cutting-edge, Zygotic Genome Activation: Methods and Protocols is a valuable resource for scientists and researchers who are interested in learning more about practical approaches to monitor the changes seen during ZGA. |
| Beschreibung: | 1 Online-Ressource (XI, 272 p. 51 illus., 41 illus. in color) |
| ISBN: | 9781493969883 |
| DOI: | 10.1007/978-1-4939-6988-3 |
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| spelling | Zygotic Genome Activation Methods and Protocols edited by Kiho Lee New York, NY Springer New York 2017 1 Online-Ressource (XI, 272 p. 51 illus., 41 illus. in color) txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology 1605 This volume provides an overview of zygotic genome activation (ZGA) and the use of recent tools to elucidate the events that occur during ZGA. The chapters in this book cover topics such as: clearance of maternal RNAs, detection of miRNA in mammalian oocytes and embryos, RNA FISH to study ZGA in early mouse embryos, and CRISPR/Cas9-mediated gene targeting during embryogenesis in the mouse and pig. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and cutting-edge, Zygotic Genome Activation: Methods and Protocols is a valuable resource for scientists and researchers who are interested in learning more about practical approaches to monitor the changes seen during ZGA. Biomedicine Human Genetics Medicine Human genetics Lee, Kiho edt Erscheint auch als Druck-Ausgabe 9781493969869 https://doi.org/10.1007/978-1-4939-6988-3 Verlag URL des Erstveröffentlichers Volltext |
| spellingShingle | Zygotic Genome Activation Methods and Protocols Biomedicine Human Genetics Medicine Human genetics |
| title | Zygotic Genome Activation Methods and Protocols |
| title_auth | Zygotic Genome Activation Methods and Protocols |
| title_exact_search | Zygotic Genome Activation Methods and Protocols |
| title_full | Zygotic Genome Activation Methods and Protocols edited by Kiho Lee |
| title_fullStr | Zygotic Genome Activation Methods and Protocols edited by Kiho Lee |
| title_full_unstemmed | Zygotic Genome Activation Methods and Protocols edited by Kiho Lee |
| title_short | Zygotic Genome Activation |
| title_sort | zygotic genome activation methods and protocols |
| title_sub | Methods and Protocols |
| topic | Biomedicine Human Genetics Medicine Human genetics |
| topic_facet | Biomedicine Human Genetics Medicine Human genetics |
| url | https://doi.org/10.1007/978-1-4939-6988-3 |
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