Hydrocarbon and Lipid Microbiology Protocols: Single-Cell and Single-Molecule Methods
This Volume presents relevant single-cell and single-molecule approaches in the study of microbes producing and utilizing hydrocarbons and lipids. While generically applicable for all microorganisms, the approaches described are, wherever possible, adapted to the field of study of hydrocarbon and li...
Gespeichert in:
| Weitere Verfasser: | , , |
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| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
Berlin, Heidelberg
Springer Berlin Heidelberg
2016
|
| Ausgabe: | 1st ed. 2016 |
| Schriftenreihe: | Springer Protocols Handbooks
|
| Schlagworte: | |
| Online-Zugang: | UBR01 TUM01 URL des Erstveröffentlichers |
| Zusammenfassung: | This Volume presents relevant single-cell and single-molecule approaches in the study of microbes producing and utilizing hydrocarbons and lipids. While generically applicable for all microorganisms, the approaches described are, wherever possible, adapted to the field of study of hydrocarbon and lipid microbiology. The methods include basic procedures for isolating single cells by means of microfluidics and flow cytometry, and their cultivation in arrays as pure clones; for isolating, amplifying and sequencing single-cell genomes and transcriptomes; and for analysing single-cell metabolomes by means of Raman spectroscopy. Single-molecule approaches include the use of protein:fluorescent dye fusions for protein localization and methods for the production of cell division protostructures and lipid monolayers. Methods for the functional analysis of single cells include detection of metabolically active (protein-synthesizing) cells in environmental samples by bioorthogonal non-canonical amino acid tagging, Raman spectroscopy combined with stable isotope labelling and fluorescent in situ hybridisation, and visualization of single cells participating in gene transfer activity. Lastly, protocols are presented for single-cell biotechnological applications, including biofuel production. Hydrocarbon and Lipid Microbiology Protocols There are tens of thousands of structurally different hydrocarbons, hydrocarbon derivatives and lipids, and a wide array of these molecules are required for cells to function. The global hydrocarbon cycle, which is largely driven by microorganisms, has a major impact on our environment and climate. Microbes are responsible for cleaning up the environmental pollution caused by the exploitation of hydrocarbon reservoirs and will also be pivotal in reducing our reliance on fossil fuels by providing biofuels, plastics and industrial chemicals. Gaining an understanding of the relevant functions of the wide range of microbes that produce, consume and modify hydrocarbons and related compounds will be key to responding to these challenges. This comprehensive collection of current and emerging protocols will facilitate acquisition of this understanding and exploitation of useful activities of such microbes |
| Beschreibung: | 1 Online-Ressource (X, 204 p. 40 illus., 20 illus. in color) |
| ISBN: | 9783662491317 |
| DOI: | 10.1007/978-3-662-49131-7 |
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| 520 | |a Methods for the functional analysis of single cells include detection of metabolically active (protein-synthesizing) cells in environmental samples by bioorthogonal non-canonical amino acid tagging, Raman spectroscopy combined with stable isotope labelling and fluorescent in situ hybridisation, and visualization of single cells participating in gene transfer activity. Lastly, protocols are presented for single-cell biotechnological applications, including biofuel production. Hydrocarbon and Lipid Microbiology Protocols There are tens of thousands of structurally different hydrocarbons, hydrocarbon derivatives and lipids, and a wide array of these molecules are required for cells to function. The global hydrocarbon cycle, which is largely driven by microorganisms, has a major impact on our environment and climate. | ||
| 520 | |a Microbes are responsible for cleaning up the environmental pollution caused by the exploitation of hydrocarbon reservoirs and will also be pivotal in reducing our reliance on fossil fuels by providing biofuels, plastics and industrial chemicals. Gaining an understanding of the relevant functions of the wide range of microbes that produce, consume and modify hydrocarbons and related compounds will be key to responding to these challenges. This comprehensive collection of current and emerging protocols will facilitate acquisition of this understanding and exploitation of useful activities of such microbes | ||
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Datensatz im Suchindex
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| dewey-ones | 579 - Microorganisms, fungi & algae |
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| dewey-tens | 570 - Biology |
| discipline | Biologie |
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| edition | 1st ed. 2016 |
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| spelling | Hydrocarbon and Lipid Microbiology Protocols Single-Cell and Single-Molecule Methods edited by Terry J. McGenity, Kenneth N. Timmis, Balbina Nogales 1st ed. 2016 Berlin, Heidelberg Springer Berlin Heidelberg 2016 1 Online-Ressource (X, 204 p. 40 illus., 20 illus. in color) txt rdacontent c rdamedia cr rdacarrier Springer Protocols Handbooks This Volume presents relevant single-cell and single-molecule approaches in the study of microbes producing and utilizing hydrocarbons and lipids. While generically applicable for all microorganisms, the approaches described are, wherever possible, adapted to the field of study of hydrocarbon and lipid microbiology. The methods include basic procedures for isolating single cells by means of microfluidics and flow cytometry, and their cultivation in arrays as pure clones; for isolating, amplifying and sequencing single-cell genomes and transcriptomes; and for analysing single-cell metabolomes by means of Raman spectroscopy. Single-molecule approaches include the use of protein:fluorescent dye fusions for protein localization and methods for the production of cell division protostructures and lipid monolayers. Methods for the functional analysis of single cells include detection of metabolically active (protein-synthesizing) cells in environmental samples by bioorthogonal non-canonical amino acid tagging, Raman spectroscopy combined with stable isotope labelling and fluorescent in situ hybridisation, and visualization of single cells participating in gene transfer activity. Lastly, protocols are presented for single-cell biotechnological applications, including biofuel production. Hydrocarbon and Lipid Microbiology Protocols There are tens of thousands of structurally different hydrocarbons, hydrocarbon derivatives and lipids, and a wide array of these molecules are required for cells to function. The global hydrocarbon cycle, which is largely driven by microorganisms, has a major impact on our environment and climate. Microbes are responsible for cleaning up the environmental pollution caused by the exploitation of hydrocarbon reservoirs and will also be pivotal in reducing our reliance on fossil fuels by providing biofuels, plastics and industrial chemicals. Gaining an understanding of the relevant functions of the wide range of microbes that produce, consume and modify hydrocarbons and related compounds will be key to responding to these challenges. This comprehensive collection of current and emerging protocols will facilitate acquisition of this understanding and exploitation of useful activities of such microbes Life Sciences Microbiology Biochemical Engineering Life sciences Biochemical engineering Kohlenwasserstoffe (DE-588)4125165-9 gnd rswk-swf Mikrobiologie (DE-588)4316357-9 gnd rswk-swf Lipide (DE-588)4035873-2 gnd rswk-swf Mikroskopie (DE-588)4039238-7 gnd rswk-swf 1\p (DE-588)4143413-4 Aufsatzsammlung gnd-content Mikrobiologie (DE-588)4316357-9 s Kohlenwasserstoffe (DE-588)4125165-9 s Lipide (DE-588)4035873-2 s Mikroskopie (DE-588)4039238-7 s 2\p DE-604 McGenity, Terry J. edt Timmis, Kenneth N. edt Nogales, Balbina edt Erscheint auch als Druck-Ausgabe 9783662491294 https://doi.org/10.1007/978-3-662-49131-7 Verlag URL des Erstveröffentlichers Volltext 1\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk 2\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk |
| spellingShingle | Hydrocarbon and Lipid Microbiology Protocols Single-Cell and Single-Molecule Methods Life Sciences Microbiology Biochemical Engineering Life sciences Biochemical engineering Kohlenwasserstoffe (DE-588)4125165-9 gnd Mikrobiologie (DE-588)4316357-9 gnd Lipide (DE-588)4035873-2 gnd Mikroskopie (DE-588)4039238-7 gnd |
| subject_GND | (DE-588)4125165-9 (DE-588)4316357-9 (DE-588)4035873-2 (DE-588)4039238-7 (DE-588)4143413-4 |
| title | Hydrocarbon and Lipid Microbiology Protocols Single-Cell and Single-Molecule Methods |
| title_auth | Hydrocarbon and Lipid Microbiology Protocols Single-Cell and Single-Molecule Methods |
| title_exact_search | Hydrocarbon and Lipid Microbiology Protocols Single-Cell and Single-Molecule Methods |
| title_full | Hydrocarbon and Lipid Microbiology Protocols Single-Cell and Single-Molecule Methods edited by Terry J. McGenity, Kenneth N. Timmis, Balbina Nogales |
| title_fullStr | Hydrocarbon and Lipid Microbiology Protocols Single-Cell and Single-Molecule Methods edited by Terry J. McGenity, Kenneth N. Timmis, Balbina Nogales |
| title_full_unstemmed | Hydrocarbon and Lipid Microbiology Protocols Single-Cell and Single-Molecule Methods edited by Terry J. McGenity, Kenneth N. Timmis, Balbina Nogales |
| title_short | Hydrocarbon and Lipid Microbiology Protocols |
| title_sort | hydrocarbon and lipid microbiology protocols single cell and single molecule methods |
| title_sub | Single-Cell and Single-Molecule Methods |
| topic | Life Sciences Microbiology Biochemical Engineering Life sciences Biochemical engineering Kohlenwasserstoffe (DE-588)4125165-9 gnd Mikrobiologie (DE-588)4316357-9 gnd Lipide (DE-588)4035873-2 gnd Mikroskopie (DE-588)4039238-7 gnd |
| topic_facet | Life Sciences Microbiology Biochemical Engineering Life sciences Biochemical engineering Kohlenwasserstoffe Mikrobiologie Lipide Mikroskopie Aufsatzsammlung |
| url | https://doi.org/10.1007/978-3-662-49131-7 |
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