Plant Functional Genomics: Methods and Protocols
This second edition volume discusses the revolutionary development of faster and less expensive DNA sequencing technologies from the past 10 years and focuses on general technologies that can be utilized by a wide array of plant biologists to address specific questions in their favorite model system...
Gespeichert in:
| Weitere Verfasser: | , |
|---|---|
| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
New York, NY
Springer New York
2015
|
| Ausgabe: | 2nd ed. 2015 |
| Schriftenreihe: | Methods in Molecular Biology
1284 |
| Schlagworte: | |
| Online-Zugang: | UBR01 TUM01 Volltext |
| Zusammenfassung: | This second edition volume discusses the revolutionary development of faster and less expensive DNA sequencing technologies from the past 10 years and focuses on general technologies that can be utilized by a wide array of plant biologists to address specific questions in their favorite model systems. This book is organized into five parts. Part I examines the tools and methods required for identifying epigenetic and conformational changes at the whole-genome level. Part II presents approaches used to determine key aspects of a gene’s function, such as techniques used to identify and characterize gene regulatory networks. This is followed by a discussion of tools used to analyze the levels of mRNA, mRNA translation rates, and metabolites. Part III features a compilation of forward and reverse genetic approaches that include recent implementation of high-throughput sequencing in classical methodologies such as QTL mapping. The final two parts explore strategies to facilitate and accelerate the generation and testing of functional DNA elements, and basic computational tools used to facilitate the use of systems biology approached by a broad spectrum of plant researchers. Written in the highly successful Methods of Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Practical and timely, Plant Functional Genomics: Methods and Protocols, Second Edition highlights the latest developments in DNA sequencing technologies that are likely to continue shaping the future of functional genomics |
| Beschreibung: | 1 Online-Ressource (XV, 532 p. 85 illus., 64 illus. in color) |
| ISBN: | 9781493924448 |
| DOI: | 10.1007/978-1-4939-2444-8 |
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Datensatz im Suchindex
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| dewey-full | 580 |
| dewey-hundreds | 500 - Natural sciences and mathematics |
| dewey-ones | 580 - Plants |
| dewey-raw | 580 |
| dewey-search | 580 |
| dewey-sort | 3580 |
| dewey-tens | 580 - Plants |
| discipline | Biologie |
| doi_str_mv | 10.1007/978-1-4939-2444-8 |
| edition | 2nd ed. 2015 |
| format | Electronic eBook |
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| spelling | Plant Functional Genomics Methods and Protocols edited by Jose M. Alonso, Anna N. Stepanova 2nd ed. 2015 New York, NY Springer New York 2015 1 Online-Ressource (XV, 532 p. 85 illus., 64 illus. in color) txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology 1284 This second edition volume discusses the revolutionary development of faster and less expensive DNA sequencing technologies from the past 10 years and focuses on general technologies that can be utilized by a wide array of plant biologists to address specific questions in their favorite model systems. This book is organized into five parts. Part I examines the tools and methods required for identifying epigenetic and conformational changes at the whole-genome level. Part II presents approaches used to determine key aspects of a gene’s function, such as techniques used to identify and characterize gene regulatory networks. This is followed by a discussion of tools used to analyze the levels of mRNA, mRNA translation rates, and metabolites. Part III features a compilation of forward and reverse genetic approaches that include recent implementation of high-throughput sequencing in classical methodologies such as QTL mapping. The final two parts explore strategies to facilitate and accelerate the generation and testing of functional DNA elements, and basic computational tools used to facilitate the use of systems biology approached by a broad spectrum of plant researchers. Written in the highly successful Methods of Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Practical and timely, Plant Functional Genomics: Methods and Protocols, Second Edition highlights the latest developments in DNA sequencing technologies that are likely to continue shaping the future of functional genomics Life Sciences Plant Sciences Life sciences Plant science Botany Pflanzen (DE-588)4045539-7 gnd rswk-swf Genanalyse (DE-588)4200230-8 gnd rswk-swf 1\p (DE-588)4143413-4 Aufsatzsammlung gnd-content Pflanzen (DE-588)4045539-7 s Genanalyse (DE-588)4200230-8 s 2\p DE-604 Alonso, Jose M. edt Stepanova, Anna N. edt Erscheint auch als Druck-Ausgabe 9781493924431 https://doi.org/10.1007/978-1-4939-2444-8 Verlag URL des Erstveröffentlichers Volltext 1\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk 2\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk |
| spellingShingle | Plant Functional Genomics Methods and Protocols Life Sciences Plant Sciences Life sciences Plant science Botany Pflanzen (DE-588)4045539-7 gnd Genanalyse (DE-588)4200230-8 gnd |
| subject_GND | (DE-588)4045539-7 (DE-588)4200230-8 (DE-588)4143413-4 |
| title | Plant Functional Genomics Methods and Protocols |
| title_auth | Plant Functional Genomics Methods and Protocols |
| title_exact_search | Plant Functional Genomics Methods and Protocols |
| title_full | Plant Functional Genomics Methods and Protocols edited by Jose M. Alonso, Anna N. Stepanova |
| title_fullStr | Plant Functional Genomics Methods and Protocols edited by Jose M. Alonso, Anna N. Stepanova |
| title_full_unstemmed | Plant Functional Genomics Methods and Protocols edited by Jose M. Alonso, Anna N. Stepanova |
| title_short | Plant Functional Genomics |
| title_sort | plant functional genomics methods and protocols |
| title_sub | Methods and Protocols |
| topic | Life Sciences Plant Sciences Life sciences Plant science Botany Pflanzen (DE-588)4045539-7 gnd Genanalyse (DE-588)4200230-8 gnd |
| topic_facet | Life Sciences Plant Sciences Life sciences Plant science Botany Pflanzen Genanalyse Aufsatzsammlung |
| url | https://doi.org/10.1007/978-1-4939-2444-8 |
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