Vaccinia Virus and Poxvirology: Methods and Protocols
Recombinant vaccinia viruses have become important in recent years, both as protein expression vectors, and-in the age of bioterrorism-as powerful tools for developing new smallpox vaccines, diagnostics, and understanding of poxvirus pathogenesis. In Vaccinia Virus and Poxvirology: Methods and Proto...
Gespeichert in:
Weitere Verfasser: | |
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Format: | Elektronisch E-Book |
Sprache: | English |
Veröffentlicht: |
Totowa, NJ
Humana Press
2004
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Schriftenreihe: | Methods in Molecular Biology
269 |
Schlagworte: | |
Online-Zugang: | UBR01 TUM01 Volltext |
Zusammenfassung: | Recombinant vaccinia viruses have become important in recent years, both as protein expression vectors, and-in the age of bioterrorism-as powerful tools for developing new smallpox vaccines, diagnostics, and understanding of poxvirus pathogenesis. In Vaccinia Virus and Poxvirology: Methods and Protocols, internationally recognized experts and leaders in poxvirology describe in step-by-step detail the successful techniques they have perfected to study and work with vaccinia virus and other poxviruses. They offer cutting-edge techniques for the construction and characterization of recombinant viruses; for the study of poxvirus gene transcription and DNA replication; and for the use of the virus in immunologic and cell-cell fusion assays. Other methods cover poxvirus bioinformatics, aspects of viral pathogenesis at both a protein and an animal model level, and the study of immune responses to poxvirus-the latter critical to the important role of vaccinia virus in smallpox vaccination and its potential role as a vaccine vector directed against infectious agents and cancer. The protocols presented follow the successful Methods in Molecular Biology™ series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and timely, Vaccinia Virus and Poxvirology: Methods and Protocols offers virologists and vaccinologists an unequaled source of proven techniques for understanding poxviruses and developing defenses against their use as bioterrorist agents |
Beschreibung: | 1 Online-Ressource (XX, 396 p) |
ISBN: | 9781592597895 |
DOI: | 10.1385/1592597890 |
Internformat
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spelling | Vaccinia Virus and Poxvirology Methods and Protocols edited by Stuart N. Isaacs Totowa, NJ Humana Press 2004 1 Online-Ressource (XX, 396 p) txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology 269 Recombinant vaccinia viruses have become important in recent years, both as protein expression vectors, and-in the age of bioterrorism-as powerful tools for developing new smallpox vaccines, diagnostics, and understanding of poxvirus pathogenesis. In Vaccinia Virus and Poxvirology: Methods and Protocols, internationally recognized experts and leaders in poxvirology describe in step-by-step detail the successful techniques they have perfected to study and work with vaccinia virus and other poxviruses. They offer cutting-edge techniques for the construction and characterization of recombinant viruses; for the study of poxvirus gene transcription and DNA replication; and for the use of the virus in immunologic and cell-cell fusion assays. Other methods cover poxvirus bioinformatics, aspects of viral pathogenesis at both a protein and an animal model level, and the study of immune responses to poxvirus-the latter critical to the important role of vaccinia virus in smallpox vaccination and its potential role as a vaccine vector directed against infectious agents and cancer. The protocols presented follow the successful Methods in Molecular Biology™ series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and timely, Vaccinia Virus and Poxvirology: Methods and Protocols offers virologists and vaccinologists an unequaled source of proven techniques for understanding poxviruses and developing defenses against their use as bioterrorist agents Life Sciences Microbiology Life sciences Molekulargenetik (DE-588)4039987-4 gnd rswk-swf Vaccinia-Virus (DE-588)4187306-3 gnd rswk-swf Labortechnik (DE-588)4123602-6 gnd rswk-swf 1\p (DE-588)4143413-4 Aufsatzsammlung gnd-content Vaccinia-Virus (DE-588)4187306-3 s Molekulargenetik (DE-588)4039987-4 s Labortechnik (DE-588)4123602-6 s b DE-604 Isaacs, Stuart N. edt Erscheint auch als Druck-Ausgabe 9781588292292 https://doi.org/10.1385/1592597890 Verlag URL des Erstveröffentlichers Volltext 1\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk |
spellingShingle | Vaccinia Virus and Poxvirology Methods and Protocols Life Sciences Microbiology Life sciences Molekulargenetik (DE-588)4039987-4 gnd Vaccinia-Virus (DE-588)4187306-3 gnd Labortechnik (DE-588)4123602-6 gnd |
subject_GND | (DE-588)4039987-4 (DE-588)4187306-3 (DE-588)4123602-6 (DE-588)4143413-4 |
title | Vaccinia Virus and Poxvirology Methods and Protocols |
title_auth | Vaccinia Virus and Poxvirology Methods and Protocols |
title_exact_search | Vaccinia Virus and Poxvirology Methods and Protocols |
title_full | Vaccinia Virus and Poxvirology Methods and Protocols edited by Stuart N. Isaacs |
title_fullStr | Vaccinia Virus and Poxvirology Methods and Protocols edited by Stuart N. Isaacs |
title_full_unstemmed | Vaccinia Virus and Poxvirology Methods and Protocols edited by Stuart N. Isaacs |
title_short | Vaccinia Virus and Poxvirology |
title_sort | vaccinia virus and poxvirology methods and protocols |
title_sub | Methods and Protocols |
topic | Life Sciences Microbiology Life sciences Molekulargenetik (DE-588)4039987-4 gnd Vaccinia-Virus (DE-588)4187306-3 gnd Labortechnik (DE-588)4123602-6 gnd |
topic_facet | Life Sciences Microbiology Life sciences Molekulargenetik Vaccinia-Virus Labortechnik Aufsatzsammlung |
url | https://doi.org/10.1385/1592597890 |
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