Site-Specific Recombinases: Methods and Protocols

This books aims to provide an up-to-date guide to using recombinases in the laboratory.  Chapters guide the reader through construction of transgenic animals, recombinase-mediated cassette exchange (RMCE), spatial control of recombinase expression and in vivo delivery approaches, immunohistochemistr...

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Weitere Verfasser: Eroshenko, Nikolai (HerausgeberIn)
Format: Elektronisch E-Book
Sprache:English
Veröffentlicht: New York, NY Springer New York 2017
Schriftenreihe:Methods in Molecular Biology 1642
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Zusammenfassung:This books aims to provide an up-to-date guide to using recombinases in the laboratory.  Chapters guide the reader through construction of transgenic animals, recombinase-mediated cassette exchange (RMCE), spatial control of recombinase expression and in vivo delivery approaches, immunohistochemistry and fluorescence-based phenotyping methods, biochemical methods, and recombinase-based production of minimal DNA vectors. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Site-Specific Recombinases: Methods and Protocols aims to serve as a valuable resource for working with recombinases, and inspires further creativity in the field
Beschreibung:1 Online-Ressource (XI, 342 p. 50 illus., 25 illus. in color)
ISBN:9781493971695
DOI:10.1007/978-1-4939-7169-5

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