ELISA: Methods and Protocols
This volume is a practical biochemical guide to the Enzyme-Linked Immunosorbent Assay (ELISA), used to detect a target substance in a liquid sample. The ELISA is an important and widely used diagnostic tool in medicine, animal health, botany and quality assurance processes in food and beverage prod...
Gespeichert in:
| Weitere Verfasser: | |
|---|---|
| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
New York, NY
Springer New York
2015
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| Schriftenreihe: | Methods in Molecular Biology
1318 |
| Schlagworte: | |
| Online-Zugang: | UBR01 TUM01 Volltext |
| Zusammenfassung: | This volume is a practical biochemical guide to the Enzyme-Linked Immunosorbent Assay (ELISA), used to detect a target substance in a liquid sample. The ELISA is an important and widely used diagnostic tool in medicine, animal health, botany and quality assurance processes in food and beverage production. An introductory chapter orients the reader on the basic structure and function of immunoglobulins and their fragments while subsequent chapters outline the methodology to generate monoclonal antibodies using hybridoma technology and the general methods used to purify antibodies. Multiple chapters demonstrate how to creatively use the properties of the antibody to identify, localize and quantify target analytes to answer questions and resolve problems. The reader will learn how to use a variety of immunoassay strategies, reporters and detection systems that will undoubtedly facilitate their efforts to gain answers to their own questions. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, ELISA: Methods and Protocols seeks to provide both professionals and novices with the technical information necessary for the reader to successfully use the immunoassay as part of the discovery process |
| Beschreibung: | 1 Online-Ressource (X, 216 p. 55 illus., 46 illus. in color) |
| ISBN: | 9781493927425 |
| DOI: | 10.1007/978-1-4939-2742-5 |
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| discipline | Biologie Medizin |
| doi_str_mv | 10.1007/978-1-4939-2742-5 |
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| spelling | ELISA Methods and Protocols edited by Robert Hnasko New York, NY Springer New York 2015 1 Online-Ressource (X, 216 p. 55 illus., 46 illus. in color) txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology 1318 This volume is a practical biochemical guide to the Enzyme-Linked Immunosorbent Assay (ELISA), used to detect a target substance in a liquid sample. The ELISA is an important and widely used diagnostic tool in medicine, animal health, botany and quality assurance processes in food and beverage production. An introductory chapter orients the reader on the basic structure and function of immunoglobulins and their fragments while subsequent chapters outline the methodology to generate monoclonal antibodies using hybridoma technology and the general methods used to purify antibodies. Multiple chapters demonstrate how to creatively use the properties of the antibody to identify, localize and quantify target analytes to answer questions and resolve problems. The reader will learn how to use a variety of immunoassay strategies, reporters and detection systems that will undoubtedly facilitate their efforts to gain answers to their own questions. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, ELISA: Methods and Protocols seeks to provide both professionals and novices with the technical information necessary for the reader to successfully use the immunoassay as part of the discovery process Biomedicine Immunology Medicine Enzyme-linked immunosorbent assay (DE-588)4152476-7 gnd rswk-swf Methode (DE-588)4038971-6 gnd rswk-swf Enzyme-linked immunosorbent assay (DE-588)4152476-7 s Methode (DE-588)4038971-6 s 1\p DE-604 Hnasko, Robert edt Erscheint auch als Druck-Ausgabe 9781493927418 https://doi.org/10.1007/978-1-4939-2742-5 Verlag URL des Erstveröffentlichers Volltext 1\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk |
| spellingShingle | ELISA Methods and Protocols Biomedicine Immunology Medicine Enzyme-linked immunosorbent assay (DE-588)4152476-7 gnd Methode (DE-588)4038971-6 gnd |
| subject_GND | (DE-588)4152476-7 (DE-588)4038971-6 |
| title | ELISA Methods and Protocols |
| title_auth | ELISA Methods and Protocols |
| title_exact_search | ELISA Methods and Protocols |
| title_full | ELISA Methods and Protocols edited by Robert Hnasko |
| title_fullStr | ELISA Methods and Protocols edited by Robert Hnasko |
| title_full_unstemmed | ELISA Methods and Protocols edited by Robert Hnasko |
| title_short | ELISA |
| title_sort | elisa methods and protocols |
| title_sub | Methods and Protocols |
| topic | Biomedicine Immunology Medicine Enzyme-linked immunosorbent assay (DE-588)4152476-7 gnd Methode (DE-588)4038971-6 gnd |
| topic_facet | Biomedicine Immunology Medicine Enzyme-linked immunosorbent assay Methode |
| url | https://doi.org/10.1007/978-1-4939-2742-5 |
| work_keys_str_mv | AT hnaskorobert elisamethodsandprotocols |