Trinucleotide Repeat Protocols:
The discovery that trinucleotide repeats significantly influence the age of onset and severity of a variety of hereditary neurological disorders has opened the door to a deeper understanding of the disease mechanisms involved, as well as to a more productive search for novel therapeutic intervention...
Gespeichert in:
| Weitere Verfasser: | |
|---|---|
| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
Totowa, NJ
Humana Press
2004
|
| Schriftenreihe: | Methods in Molecular Biology™
277 |
| Schlagworte: | |
| Online-Zugang: | UBR01 TUM01 Volltext |
| Zusammenfassung: | The discovery that trinucleotide repeats significantly influence the age of onset and severity of a variety of hereditary neurological disorders has opened the door to a deeper understanding of the disease mechanisms involved, as well as to a more productive search for novel therapeutic interventions. In Trinucleotide Repeat Protocols, established leaders in trinucleotide repeat disease describe in step-by-step detail their best techniques for studying trinucleotide pathology at the molecular level. The protocols cover a variety of targets, ranging from DNA and RNA to proteins and whole animals, and focus not only on causal genes, but also on their consequent products, such as transcription factors, neurotransmitter receptors, proteasomes, and mitochondria/oxidation damage. Experimental systems employed include E. coli, yeast, C. elegans, mouse, and generally take a clinical point of view. The authors utilize a wide range of techniques, including gel electrophoresis, quantitative RT-PCR, immunological analysis, antibody usage and its applications, receptor assays using radioisotope handling, gene delivery by virus, brain cell and organotypic cultures, gender dependency, and neuron structure analysis. Each protocol follows the successful Methods in Molecular Biology™ series format, offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and highly practical, Trinucleotide Repeat Protocols offers neuroscientists powerful tools to elucidate both normal brain function and the mechanisms of hereditary neurological disease, as well as to develop the next generation of therapies for neuronal genetic diseases |
| Beschreibung: | 1 Online-Ressource (XII, 342 p) |
| ISBN: | 9781592598045 |
| DOI: | 10.1385/1592598048 |
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| spelling | Trinucleotide Repeat Protocols edited by Yoshinori Kohwi Totowa, NJ Humana Press 2004 1 Online-Ressource (XII, 342 p) txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology™ 277 The discovery that trinucleotide repeats significantly influence the age of onset and severity of a variety of hereditary neurological disorders has opened the door to a deeper understanding of the disease mechanisms involved, as well as to a more productive search for novel therapeutic interventions. In Trinucleotide Repeat Protocols, established leaders in trinucleotide repeat disease describe in step-by-step detail their best techniques for studying trinucleotide pathology at the molecular level. The protocols cover a variety of targets, ranging from DNA and RNA to proteins and whole animals, and focus not only on causal genes, but also on their consequent products, such as transcription factors, neurotransmitter receptors, proteasomes, and mitochondria/oxidation damage. Experimental systems employed include E. coli, yeast, C. elegans, mouse, and generally take a clinical point of view. The authors utilize a wide range of techniques, including gel electrophoresis, quantitative RT-PCR, immunological analysis, antibody usage and its applications, receptor assays using radioisotope handling, gene delivery by virus, brain cell and organotypic cultures, gender dependency, and neuron structure analysis. Each protocol follows the successful Methods in Molecular Biology™ series format, offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and highly practical, Trinucleotide Repeat Protocols offers neuroscientists powerful tools to elucidate both normal brain function and the mechanisms of hereditary neurological disease, as well as to develop the next generation of therapies for neuronal genetic diseases Life Sciences Cell Biology Life sciences Cell biology Labortechnik (DE-588)4123602-6 gnd rswk-swf Trinucleotide (DE-588)4595717-4 gnd rswk-swf 1\p (DE-588)4143413-4 Aufsatzsammlung gnd-content Trinucleotide (DE-588)4595717-4 s Labortechnik (DE-588)4123602-6 s b DE-604 Kohwi, Yoshinori edt Erscheint auch als Druck-Ausgabe 9781588292438 https://doi.org/10.1385/1592598048 Verlag URL des Erstveröffentlichers Volltext 1\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk |
| spellingShingle | Trinucleotide Repeat Protocols Life Sciences Cell Biology Life sciences Cell biology Labortechnik (DE-588)4123602-6 gnd Trinucleotide (DE-588)4595717-4 gnd |
| subject_GND | (DE-588)4123602-6 (DE-588)4595717-4 (DE-588)4143413-4 |
| title | Trinucleotide Repeat Protocols |
| title_auth | Trinucleotide Repeat Protocols |
| title_exact_search | Trinucleotide Repeat Protocols |
| title_full | Trinucleotide Repeat Protocols edited by Yoshinori Kohwi |
| title_fullStr | Trinucleotide Repeat Protocols edited by Yoshinori Kohwi |
| title_full_unstemmed | Trinucleotide Repeat Protocols edited by Yoshinori Kohwi |
| title_short | Trinucleotide Repeat Protocols |
| title_sort | trinucleotide repeat protocols |
| topic | Life Sciences Cell Biology Life sciences Cell biology Labortechnik (DE-588)4123602-6 gnd Trinucleotide (DE-588)4595717-4 gnd |
| topic_facet | Life Sciences Cell Biology Life sciences Cell biology Labortechnik Trinucleotide Aufsatzsammlung |
| url | https://doi.org/10.1385/1592598048 |
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