In Vitro Transcription and Translation Protocols:
Most laboratories conducting studies that use molecular biology techniques employ in vitro transcription and translation systems as a routine part of their day-to-day research. The commercial availability of purified bacterial RNA polymerase and the availability of robust tra- lation systems has mad...
Gespeichert in:
| Weitere Verfasser: | |
|---|---|
| Format: | Elektronisch E-Book |
| Sprache: | English |
| Veröffentlicht: |
Totowa, NJ
Humana Press
1995
|
| Schriftenreihe: | Methods in Molecular Biology
37 |
| Schlagworte: | |
| Online-Zugang: | UBR01 Volltext |
| Zusammenfassung: | Most laboratories conducting studies that use molecular biology techniques employ in vitro transcription and translation systems as a routine part of their day-to-day research. The commercial availability of purified bacterial RNA polymerase and the availability of robust tra- lation systems has made in vitro systems attractive not only as an alt- native to the in vivo expression of genes, but also as good model systems for studying specific aspects of transcription and translation. Although fairly efficient eukaryotic translation systems have been established for a number of years, reconstitution of transcription in vitro has proved to be more difficult. Recent improvements in fractionation techniques and the cloning of proteins involved in transcription have made this a fast moving area of research. Considerable progress has also been made in recent years in developing in vitro systems to study transcription and translation in chloroplasts and mitochondria, together with systems for the study of protein import. In Vitro Transcription and Translation Protocols provides many detailed experimental procedures for prokaryotic transcription and translation systems, together with protocols for many key techniques used in the analysis of eukaryotic transcription. In keeping with the successful format of preceding volumes of the Methods in Molecular Biology series, step-by-step instructions are provided, together with extensive notes that cover troubleshooting and special tips considered important |
| Beschreibung: | 1 Online-Ressource (XII, 432 p) |
| ISBN: | 9781592595242 |
| DOI: | 10.1385/0896032884 |
Internformat
MARC
| LEADER | 00000nmm a2200000zcb4500 | ||
|---|---|---|---|
| 001 | BV044950495 | ||
| 003 | DE-604 | ||
| 005 | 20180829 | ||
| 007 | cr|uuu---uuuuu | ||
| 008 | 180517s1995 |||| o||u| ||||||eng d | ||
| 020 | |a 9781592595242 |9 978-1-59259-524-2 | ||
| 024 | 7 | |a 10.1385/0896032884 |2 doi | |
| 035 | |a (ZDB-2-PRO)978-1-59259-524-2 | ||
| 035 | |a (OCoLC)1036376634 | ||
| 035 | |a (DE-599)BVBBV044950495 | ||
| 040 | |a DE-604 |b ger |e rda | ||
| 041 | 0 | |a eng | |
| 049 | |a DE-355 | ||
| 050 | 0 | |a QH442 | |
| 082 | 0 | |a 574.87/3223 |2 20 | |
| 082 | 0 | |a 571.6 |2 23 | |
| 084 | |a WC 4400 |0 (DE-625)148099: |2 rvk | ||
| 084 | |a WC 4460 |0 (DE-625)148102: |2 rvk | ||
| 084 | |a WG 1800 |0 (DE-625)148501: |2 rvk | ||
| 084 | |a WG 1850 |0 (DE-625)148505: |2 rvk | ||
| 245 | 1 | 0 | |a In Vitro Transcription and Translation Protocols |c edited by Martin J. Tymms |
| 264 | 1 | |a Totowa, NJ |b Humana Press |c 1995 | |
| 300 | |a 1 Online-Ressource (XII, 432 p) | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b c |2 rdamedia | ||
| 338 | |b cr |2 rdacarrier | ||
| 490 | 0 | |a Methods in Molecular Biology |v 37 | |
| 520 | |a Most laboratories conducting studies that use molecular biology techniques employ in vitro transcription and translation systems as a routine part of their day-to-day research. The commercial availability of purified bacterial RNA polymerase and the availability of robust tra- lation systems has made in vitro systems attractive not only as an alt- native to the in vivo expression of genes, but also as good model systems for studying specific aspects of transcription and translation. Although fairly efficient eukaryotic translation systems have been established for a number of years, reconstitution of transcription in vitro has proved to be more difficult. Recent improvements in fractionation techniques and the cloning of proteins involved in transcription have made this a fast moving area of research. Considerable progress has also been made in recent years in developing in vitro systems to study transcription and translation in chloroplasts and mitochondria, together with systems for the study of protein import. In Vitro Transcription and Translation Protocols provides many detailed experimental procedures for prokaryotic transcription and translation systems, together with protocols for many key techniques used in the analysis of eukaryotic transcription. In keeping with the successful format of preceding volumes of the Methods in Molecular Biology series, step-by-step instructions are provided, together with extensive notes that cover troubleshooting and special tips considered important | ||
| 650 | 4 | |a Life Sciences | |
| 650 | 4 | |a Cell Biology | |
| 650 | 4 | |a Life sciences | |
| 650 | 4 | |a Cell biology | |
| 650 | 0 | 7 | |a Methode |0 (DE-588)4038971-6 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Translation |g Genetik |0 (DE-588)4185909-1 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Transkription |g Genetik |0 (DE-588)4185906-6 |2 gnd |9 rswk-swf |
| 689 | 0 | 0 | |a Transkription |g Genetik |0 (DE-588)4185906-6 |D s |
| 689 | 0 | 1 | |a Methode |0 (DE-588)4038971-6 |D s |
| 689 | 0 | |5 DE-604 | |
| 689 | 1 | 0 | |a Translation |g Genetik |0 (DE-588)4185909-1 |D s |
| 689 | 1 | 1 | |a Methode |0 (DE-588)4038971-6 |D s |
| 689 | 1 | |5 DE-604 | |
| 700 | 1 | |a Tymms, Martin J. |4 edt | |
| 776 | 0 | 8 | |i Erscheint auch als |n Druck-Ausgabe |z 9780896032880 |
| 856 | 4 | 0 | |u https://doi.org/10.1385/0896032884 |x Verlag |z URL des Erstveröffentlichers |3 Volltext |
| 912 | |a ZDB-2-PRO | ||
| 999 | |a oai:aleph.bib-bvb.de:BVB01-030343256 | ||
| 966 | e | |u https://doi.org/10.1385/0896032884 |l UBR01 |p ZDB-2-PRO |x Verlag |3 Volltext | |
Datensatz im Suchindex
| _version_ | 1804178537980100608 |
|---|---|
| any_adam_object | |
| author2 | Tymms, Martin J. |
| author2_role | edt |
| author2_variant | m j t mj mjt |
| author_facet | Tymms, Martin J. |
| building | Verbundindex |
| bvnumber | BV044950495 |
| callnumber-first | Q - Science |
| callnumber-label | QH442 |
| callnumber-raw | QH442 |
| callnumber-search | QH442 |
| callnumber-sort | QH 3442 |
| callnumber-subject | QH - Natural History and Biology |
| classification_rvk | WC 4400 WC 4460 WG 1800 WG 1850 |
| collection | ZDB-2-PRO |
| ctrlnum | (ZDB-2-PRO)978-1-59259-524-2 (OCoLC)1036376634 (DE-599)BVBBV044950495 |
| dewey-full | 574.87/3223 571.6 |
| dewey-hundreds | 500 - Natural sciences and mathematics |
| dewey-ones | 574 - [Unassigned] 571 - Physiology & related subjects |
| dewey-raw | 574.87/3223 571.6 |
| dewey-search | 574.87/3223 571.6 |
| dewey-sort | 3574.87 43223 |
| dewey-tens | 570 - Biology |
| discipline | Biologie |
| doi_str_mv | 10.1385/0896032884 |
| format | Electronic eBook |
| fullrecord | <?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>03531nmm a2200589zcb4500</leader><controlfield tag="001">BV044950495</controlfield><controlfield tag="003">DE-604</controlfield><controlfield tag="005">20180829 </controlfield><controlfield tag="007">cr|uuu---uuuuu</controlfield><controlfield tag="008">180517s1995 |||| o||u| ||||||eng d</controlfield><datafield tag="020" ind1=" " ind2=" "><subfield code="a">9781592595242</subfield><subfield code="9">978-1-59259-524-2</subfield></datafield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1385/0896032884</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(ZDB-2-PRO)978-1-59259-524-2</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(OCoLC)1036376634</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)BVBBV044950495</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-604</subfield><subfield code="b">ger</subfield><subfield code="e">rda</subfield></datafield><datafield tag="041" ind1="0" ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="049" ind1=" " ind2=" "><subfield code="a">DE-355</subfield></datafield><datafield tag="050" ind1=" " ind2="0"><subfield code="a">QH442</subfield></datafield><datafield tag="082" ind1="0" ind2=" "><subfield code="a">574.87/3223</subfield><subfield code="2">20</subfield></datafield><datafield tag="082" ind1="0" ind2=" "><subfield code="a">571.6</subfield><subfield code="2">23</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">WC 4400</subfield><subfield code="0">(DE-625)148099:</subfield><subfield code="2">rvk</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">WC 4460</subfield><subfield code="0">(DE-625)148102:</subfield><subfield code="2">rvk</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">WG 1800</subfield><subfield code="0">(DE-625)148501:</subfield><subfield code="2">rvk</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">WG 1850</subfield><subfield code="0">(DE-625)148505:</subfield><subfield code="2">rvk</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">In Vitro Transcription and Translation Protocols</subfield><subfield code="c">edited by Martin J. Tymms</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="a">Totowa, NJ</subfield><subfield code="b">Humana Press</subfield><subfield code="c">1995</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">1 Online-Ressource (XII, 432 p)</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="490" ind1="0" ind2=" "><subfield code="a">Methods in Molecular Biology</subfield><subfield code="v">37</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Most laboratories conducting studies that use molecular biology techniques employ in vitro transcription and translation systems as a routine part of their day-to-day research. The commercial availability of purified bacterial RNA polymerase and the availability of robust tra- lation systems has made in vitro systems attractive not only as an alt- native to the in vivo expression of genes, but also as good model systems for studying specific aspects of transcription and translation. Although fairly efficient eukaryotic translation systems have been established for a number of years, reconstitution of transcription in vitro has proved to be more difficult. Recent improvements in fractionation techniques and the cloning of proteins involved in transcription have made this a fast moving area of research. Considerable progress has also been made in recent years in developing in vitro systems to study transcription and translation in chloroplasts and mitochondria, together with systems for the study of protein import. In Vitro Transcription and Translation Protocols provides many detailed experimental procedures for prokaryotic transcription and translation systems, together with protocols for many key techniques used in the analysis of eukaryotic transcription. In keeping with the successful format of preceding volumes of the Methods in Molecular Biology series, step-by-step instructions are provided, together with extensive notes that cover troubleshooting and special tips considered important</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Life Sciences</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Cell Biology</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Life sciences</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Cell biology</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Methode</subfield><subfield code="0">(DE-588)4038971-6</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Translation</subfield><subfield code="g">Genetik</subfield><subfield code="0">(DE-588)4185909-1</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Transkription</subfield><subfield code="g">Genetik</subfield><subfield code="0">(DE-588)4185906-6</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="689" ind1="0" ind2="0"><subfield code="a">Transkription</subfield><subfield code="g">Genetik</subfield><subfield code="0">(DE-588)4185906-6</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2="1"><subfield code="a">Methode</subfield><subfield code="0">(DE-588)4038971-6</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2=" "><subfield code="5">DE-604</subfield></datafield><datafield tag="689" ind1="1" ind2="0"><subfield code="a">Translation</subfield><subfield code="g">Genetik</subfield><subfield code="0">(DE-588)4185909-1</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="1" ind2="1"><subfield code="a">Methode</subfield><subfield code="0">(DE-588)4038971-6</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="1" ind2=" "><subfield code="5">DE-604</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Tymms, Martin J.</subfield><subfield code="4">edt</subfield></datafield><datafield tag="776" ind1="0" ind2="8"><subfield code="i">Erscheint auch als</subfield><subfield code="n">Druck-Ausgabe</subfield><subfield code="z">9780896032880</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://doi.org/10.1385/0896032884</subfield><subfield code="x">Verlag</subfield><subfield code="z">URL des Erstveröffentlichers</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">ZDB-2-PRO</subfield></datafield><datafield tag="999" ind1=" " ind2=" "><subfield code="a">oai:aleph.bib-bvb.de:BVB01-030343256</subfield></datafield><datafield tag="966" ind1="e" ind2=" "><subfield code="u">https://doi.org/10.1385/0896032884</subfield><subfield code="l">UBR01</subfield><subfield code="p">ZDB-2-PRO</subfield><subfield code="x">Verlag</subfield><subfield code="3">Volltext</subfield></datafield></record></collection> |
| id | DE-604.BV044950495 |
| illustrated | Not Illustrated |
| indexdate | 2024-07-10T08:05:33Z |
| institution | BVB |
| isbn | 9781592595242 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-030343256 |
| oclc_num | 1036376634 |
| open_access_boolean | |
| owner | DE-355 DE-BY-UBR |
| owner_facet | DE-355 DE-BY-UBR |
| physical | 1 Online-Ressource (XII, 432 p) |
| psigel | ZDB-2-PRO |
| publishDate | 1995 |
| publishDateSearch | 1995 |
| publishDateSort | 1995 |
| publisher | Humana Press |
| record_format | marc |
| series2 | Methods in Molecular Biology |
| spelling | In Vitro Transcription and Translation Protocols edited by Martin J. Tymms Totowa, NJ Humana Press 1995 1 Online-Ressource (XII, 432 p) txt rdacontent c rdamedia cr rdacarrier Methods in Molecular Biology 37 Most laboratories conducting studies that use molecular biology techniques employ in vitro transcription and translation systems as a routine part of their day-to-day research. The commercial availability of purified bacterial RNA polymerase and the availability of robust tra- lation systems has made in vitro systems attractive not only as an alt- native to the in vivo expression of genes, but also as good model systems for studying specific aspects of transcription and translation. Although fairly efficient eukaryotic translation systems have been established for a number of years, reconstitution of transcription in vitro has proved to be more difficult. Recent improvements in fractionation techniques and the cloning of proteins involved in transcription have made this a fast moving area of research. Considerable progress has also been made in recent years in developing in vitro systems to study transcription and translation in chloroplasts and mitochondria, together with systems for the study of protein import. In Vitro Transcription and Translation Protocols provides many detailed experimental procedures for prokaryotic transcription and translation systems, together with protocols for many key techniques used in the analysis of eukaryotic transcription. In keeping with the successful format of preceding volumes of the Methods in Molecular Biology series, step-by-step instructions are provided, together with extensive notes that cover troubleshooting and special tips considered important Life Sciences Cell Biology Life sciences Cell biology Methode (DE-588)4038971-6 gnd rswk-swf Translation Genetik (DE-588)4185909-1 gnd rswk-swf Transkription Genetik (DE-588)4185906-6 gnd rswk-swf Transkription Genetik (DE-588)4185906-6 s Methode (DE-588)4038971-6 s DE-604 Translation Genetik (DE-588)4185909-1 s Tymms, Martin J. edt Erscheint auch als Druck-Ausgabe 9780896032880 https://doi.org/10.1385/0896032884 Verlag URL des Erstveröffentlichers Volltext |
| spellingShingle | In Vitro Transcription and Translation Protocols Life Sciences Cell Biology Life sciences Cell biology Methode (DE-588)4038971-6 gnd Translation Genetik (DE-588)4185909-1 gnd Transkription Genetik (DE-588)4185906-6 gnd |
| subject_GND | (DE-588)4038971-6 (DE-588)4185909-1 (DE-588)4185906-6 |
| title | In Vitro Transcription and Translation Protocols |
| title_auth | In Vitro Transcription and Translation Protocols |
| title_exact_search | In Vitro Transcription and Translation Protocols |
| title_full | In Vitro Transcription and Translation Protocols edited by Martin J. Tymms |
| title_fullStr | In Vitro Transcription and Translation Protocols edited by Martin J. Tymms |
| title_full_unstemmed | In Vitro Transcription and Translation Protocols edited by Martin J. Tymms |
| title_short | In Vitro Transcription and Translation Protocols |
| title_sort | in vitro transcription and translation protocols |
| topic | Life Sciences Cell Biology Life sciences Cell biology Methode (DE-588)4038971-6 gnd Translation Genetik (DE-588)4185909-1 gnd Transkription Genetik (DE-588)4185906-6 gnd |
| topic_facet | Life Sciences Cell Biology Life sciences Cell biology Methode Translation Genetik Transkription Genetik |
| url | https://doi.org/10.1385/0896032884 |
| work_keys_str_mv | AT tymmsmartinj invitrotranscriptionandtranslationprotocols |